Alkaline Phosphatase of Cancerous Larynx Tissue in Comparison with the Placental Enzyme: Biochemical and Histochemical Studies

Al-Mudamgha, Zainab A.; Rassam, Maysoon; Al-Salihi, Anam R.; Al-Sammeraie, Furat T.

doi:10.3109/02841869709109232

Cited by 3 publications

(1 citation statement)

References 16 publications

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“…High levels of ALP have been reported to be a tumor marker for various cancers [ 27 ]. Many types of pluripotent stem cells, as well as iPS cells, are known to have ALP activity [ 28 , 29 , 30 ]. Expression of cancer stem cell markers, including CD44, CD133, Oct3/4, and ALP activity, may be one reason the growth of our cell cultures was suppressed.…”

Section: Discussionmentioning

confidence: 99%

Establishment and characterization of a primary cell culture derived from external auditory canal squamous cell carcinoma

et al. 2021

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There are no human cancer cell lines of external auditory canal origin available for research use. This report describes the establishment of a culture condition for external auditory canal squamous cell carcinoma, derived from human tumor tissue. Successive squamous cell carcinoma colonies were dissociated by trypsin, subcultured, and maintained on a feeder layer (MMC-TIG-1-20), yielding a clonally proliferating cell culture. Two morphological types of colony were observed: (i) densely packed colonies and (ii) colonies with indistinct boundaries characterized by cell-cell complexes with fibroblast feeder cells. The SCC-like characteristics of these cells were evidenced by positivity for p53, SCCA1/2, cytokeratin and vimentin, and cancer stem cell properties were indicated by positivity for CD44, CD133, Oct3/4, and alkaline phosphatase (ALP). One of the unique properties of cell cultures is their tendency to form steric colonies in vitro on feeder layer cells. In addition, in the presence of fresh macrophages, the cells very slowly transform to break away from colonies as free cells, a process that resembles the epidermal-mesenchymal transition, whereby cell-cell interactions are weakened and migration activity is enhanced. These factors are purported to play a key role in cancer cell metastasis.

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Section: Discussionmentioning

confidence: 99%

Establishment and characterization of a primary cell culture derived from external auditory canal squamous cell carcinoma

et al. 2021

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Larynx

Burkhardt¹,

Meyer-Breiting²

1999

Spezielle Pathologische Anatomie

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Biological and genetic characterization of a newly established human external auditory canal carcinoma cell line, SCEACono2

Komune,

Sato,

Ono

et al. 2023

Sci Rep

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Squamous cell carcinoma of the external auditory canal (EACSCC) is an extraordinarily rare and aggressive malignant disease. Establishment of EACSCC cell line with robust molecular characteristics is essential for the basic and translational research of EACSCC. Here, we show the newly established EACSCC cell line SCEACono2, derived from a patient with well-to-moderately differentiated EACSCC. We analyzed histologic and genetic features of SCEACono2 hiring multiple experiments, including next-generation sequencing (NGS). Immunocytochemical staining of SCEACono2 showed positivity of p53 and SCC1/2. Furthermore, SCEACono2 exhibited a unique characteristic that cytokeratin, vimentin as well as cancer stem cell markers (CD44, CD133, ALP and Oct3/4) were positive. SCEACono2 had an ability to form tumors at the temporal lesion xenograft nude mice model. NGS revealed that SCEACono2 harbored the somatic mutations of TP53 (p.G245S) and NOTCH1 (p.A465T). RNA-seq and downstream bioinformatics analysis revealed significant enrichment of genes involved in inflammation and cell adhesion in SCEACono2 compared to SCC-9 and HSC-4. STR profiling indicated no evidence of cross-contamination. In conclusion, SCEACono2 could serves as a promising and robust research resource of EACSCC in vitro and in vivo.

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Alkaline Phosphatase of Cancerous Larynx Tissue in Comparison with the Placental Enzyme: Biochemical and Histochemical Studies

Cited by 3 publications

References 16 publications

Establishment and characterization of a primary cell culture derived from external auditory canal squamous cell carcinoma

Establishment and characterization of a primary cell culture derived from external auditory canal squamous cell carcinoma

Larynx

Biological and genetic characterization of a newly established human external auditory canal carcinoma cell line, SCEACono2

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