Alkaline phosphatase reduces hepatic and pulmonary injury in liver ischaemia–reperfusion combined with partial resection

Veen, Suzanne Q. van; Dinant, Sander; Vliet, Arlène K. van; Gulik, Thomas M. Van

doi:10.1002/bjs.5275

Cited by 17 publications

(12 citation statements)

References 33 publications

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“…In previous animal studies, promising therapeutic effects have been shown in reducing the inflammatory response with the use of intravenous alkaline phosphatase (9)(10)(11). In a clinical study in severe sepsis patients, continuous infusion of calf-intestinal alkaline phosphatase significantly improved renal function (12,13).…”

Section: Methodsmentioning

confidence: 98%

Prophylactic Treatment with Alkaline Phosphatase in Cardiac Surgery Induces Endogenous Alkaline Phosphatase Release

et al. 2012

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Document VersionPublisher's PDF, also known as Version of Record (includes final page, issue and volume numbers) Please check the document version of this publication:• A submitted manuscript is the author's version of the article upon submission and before peer-review. There can be important differences between the submitted version and the official published version of record. People interested in the research are advised to contact the author for the final version of the publication, or visit the DOI to the publisher's website.• The final author version and the galley proof are versions of the publication after peer review.• The final published version features the final layout of the paper including the volume, issue and page numbers. Link to publicationCitation for published version (APA): Kats, S., Brands, R., Hamad, M. A., Seinen, W., Scharnhorst, V., Wulkan, R. W., ... Oeveren, van, W. (2014). Prophylactic treatment with alkaline phosphatase in cardiac surgery induces endogenous alkaline phosphatase release. International Journal of Artificial Organs, 35(2), 144-151. DOI: 10.5301/ijao.5000039 General rightsCopyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.• Users may download and print one copy of any publication from the public portal for the purpose of private study or research.• You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal ? Take down policyIf you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.

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Section: Methodsmentioning

confidence: 98%

Prophylactic Treatment with Alkaline Phosphatase in Cardiac Surgery Induces Endogenous Alkaline Phosphatase Release

et al. 2012

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“…With respect to the clinical setting, the combination of hepatic I/R injury with partial liver resection (PHx) constitutes a more relevant model [7,11], in which only post-ischemic liver parenchyma is retained. This model therefore allows the assessment of true postoperative liver function, which depends on the extent of resection, the hepatocellular damage inflicted by I/R injury, and post-hepatectomy liver regeneration.…”

Section: Introductionmentioning

confidence: 99%

“…This model therefore allows the assessment of true postoperative liver function, which depends on the extent of resection, the hepatocellular damage inflicted by I/R injury, and post-hepatectomy liver regeneration. Analysis of hepatocellular damage alone is not an adequate measure of functional recovery, and should therefore be complemented by liver function-specific assays [7,11]. Although traditional biochemical liver function tests such as prothrombin time (PT) and plasma bilirubin concentration can be used to determine the extent of postoperative liver dysfunction, these tests comprise only an indirect and rather insensitive measure of liver function.…”

Section: Introductionmentioning

confidence: 99%

Quantitative Assessment of Liver Function after Ischemia-Reperfusion Injury and Partial Hepatectomy in Rats

Graaf

Heger

Spruijt

et al. 2012

Journal of Surgical Research

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“…Myeloperoxidase (MPO) activity, an index of neutrophil infiltration, was measured in liver homogenates of the median lobes of the ischemia and sham groups according to a method modified from Krawisz et al [29] as described elsewhere [29,30]. Values were normalized to the total protein concentration, and MPO activity was expressed in units per milligram of protein (U/mg).…”

Section: Methodsmentioning

confidence: 99%

Immunoglobulin M, C-Reactive Protein and Complement Activation in Rat Hepatic Ischemia-Reperfusion Injury

Diepenhorst

Graaf²,

Niessen³

et al. 2014

Eur Surg Res

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Background: Ischemia-reperfusion (I/R) models have shown that C-reactive protein (CRP) and immunoglobulin M (IgM) are involved in complement activation. Binding of CRP and IgM to damaged cell membranes initiates complement activation and aggravates I/R injury in various organs. However, the time course of CRP- and IgM-mediated complement activation and the relation to hepatocellular injury and inflammation in liver I/R are unknown. Aim: To evaluate the time course of IgM- and CRP-related complement activation and the relation to hepatocellular injury and inflammation in a hepatic I/R rat model. Methods: Male Wistar rats were allocated to (1) five groups of animals exposed to 60 min of partial ischemia (70%) induced via clamping of the left segmental portal triad, followed by 0, 3, 6, 12 or 24 h of reperfusion (n = 6 in each group); (2) five groups of sham-operated animals with corresponding reperfusion times (n = 5), and (3) a control group sacrificed before ischemia (n = 5). Hepatocellular injury, inflammatory response, rat plasma CRP and IgM levels and immunohistochemical depositions of CRP, IgM and C3 were assessed for each group. Results: Histopathological injury scores of hematoxylin and eosin sections of ischemic liver lobes demonstrated increasing values throughout the reperfusion time with a peak at 12 h. Plasma aminotransferases (alanine aminotransferase and aspartate aminotransferase) significantly increased after 3 h of reperfusion, peaking at 6 h (3,100 ± 800 U/l; p < 0.05). Hepatic neutrophil influx significantly increased from 3 to 6 h of reperfusion (p < 0.05) and demonstrated the highest value at 12 h (1.1 ± 0.2 U/mg of protein). Plasma IL-6 levels in the ischemia groups showed peak values after 6 h of reperfusion, decreasing significantly thereafter (p < 0.05). Plasma CRP values reached highest levels after 3 h of reperfusion (mean 91 ± 5% of control pool), decreasing significantly thereafter. Rat IgM concentrations in plasma did not significantly change throughout the reperfusion time. Immunohistochemical depositions of IgM, CRP and C3 in ischemic lobes demonstrated a similar pattern in time, reaching maximum values at 12 h of reperfusion. The percentages of depositions of CRP and IgM were significantly correlated [r(S) = 0.569; p < 0.001; Spearman test]. The time course of C3 and CRP depositions throughout reperfusion and C3 and IgM staining were significantly similar [r(S) = 0.797 and r(S) = 0.656, respectively; p < 0.0001; ANOVA]. Conclusions: CRP and IgM depositions demonstrate a parallel time course throughout the reperfusion to hepatocellular damage, inflammatory response and activated complement deposition in this rat hepatic I/R model. Furthermore, the time course of CRP and IgM depositions was significantly similar to that of activated complement depositions.

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Alkaline phosphatase reduces hepatic and pulmonary injury in liver ischaemia–reperfusion combined with partial resection

Abstract: BIAP attenuates hepatic and pulmonary injury after partial liver I/R and PHX.

Cited by 17 publications

References 33 publications

Prophylactic Treatment with Alkaline Phosphatase in Cardiac Surgery Induces Endogenous Alkaline Phosphatase Release

Prophylactic Treatment with Alkaline Phosphatase in Cardiac Surgery Induces Endogenous Alkaline Phosphatase Release

Quantitative Assessment of Liver Function after Ischemia-Reperfusion Injury and Partial Hepatectomy in Rats

Immunoglobulin M, C-Reactive Protein and Complement Activation in Rat Hepatic Ischemia-Reperfusion Injury

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