Quantitative Assessment of Liver Function after Ischemia-Reperfusion Injury and Partial Hepatectomy in Rats

Graaf, Wilmar de; Heger, Michal; Spruijt, Onno A.; Maas, Adrie; Bruin, Kora de; Hoekstra, Ruurdtje; Bennink, Roelof J.; Gulik, Thomas M. van

doi:10.1016/j.jss.2010.06.038

Cited by 28 publications

(20 citation statements)

References 35 publications

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“…Major parenchymal injury is reflected by an increase in circulating transaminases (AST and ALT) and reduced liver function, the latter of which is indicated by increases in prothrombin time (PT) and plasma bilirubin levels. These findings have been corroborated using quantitative liver function tests (19), which confirmed that severe I/R injury impairs liver function. Inasmuch as I/R injury also impairs liver regeneration, the ability to recover from surgery is further suppressed (19).…”

Section: Nadsupporting

confidence: 53%

“…These findings have been corroborated using quantitative liver function tests (19), which confirmed that severe I/R injury impairs liver function. Inasmuch as I/R injury also impairs liver regeneration, the ability to recover from surgery is further suppressed (19). If these effects persist, the ultimate consequence of I/R injury is postoperative liver failure, which is the most severe complication of liver surgery and is associated with high medical costs and a mortality rate of up to 88% (20).…”

Section: Nadsupporting

confidence: 53%

“…The first rationale for this finding was provided by the same study, which showed that the drop in OATP mRNA expression coincided with an increase in hepatic TNFa and IL-6 transcript levels (19). These findings are substantiated by an earlier study in rats subjected to 60 min of liver ischemia, which revealed that the I/R-induced reduction in basolateral transporter (OATP1A1, 1A4 and 1B2) expression could be reversed by depleting KCs min of partial hepatic ischemia at either 37°C or 34°C (61), the hypothermic groups consistently exhibited less liver injury than the normothermic controls at 24 h of reperfusion, which was reflected by a reduction in transaminase release, improved liver histology and less endothelial injury.…”

Section: Hypothermia and Glucose Metabolismmentioning

confidence: 97%

“…Functionality and expression of the rat homologs of these transporters (OATP1A1, 1A4, and 2B1, respectively [19]) was investigated in a rat model of partial liver ischemia combined with resection of the nonischemic liver lobes. At 24 h after 30-60 min of partial liver ischemia, hepatocyte uptake function was diminished, as reflected by a marked drop in ICG and 99 Tm-mebrofenin clearance.…”

Section: Hypothermia and Glucose Metabolismmentioning

confidence: 99%

“…Although I/R injury could account for the reduced 99 Tm-mebrofenin clearance by triggering hepatocyte necrosis, it should be noted that I/R also suppressed OATP1A1, 1A4 and 1B2 on a transcriptional level, which suggests a more refined control of transporter function during reperfusion (19). The first rationale for this finding was provided by the same study, which showed that the drop in OATP mRNA expression coincided with an increase in hepatic TNFa and IL-6 transcript levels (19).…”

Section: Hypothermia and Glucose Metabolismmentioning

confidence: 99%

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Protective Mechanisms of Hypothermia in Liver Surgery and Transplantation

Olthof

Reiniers

Dirkes

et al. 2015

Mol Med

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Hepatic ischemia/reperfusion (I/R) injury is a side effect of major liver surgery that often cannot be avoided. Prolonged periods of ischemia put a metabolic strain on hepatocytes and limit the tolerable ischemia and preservation times during liver resection and transplantation, respectively. In both surgical settings, temporarily lowering the metabolic demand of the organ by reducing organ temperature effectively counteracts the negative consequences of an ischemic insult. Despite its routine use, the application of liver cooling is predicated on an incomplete understanding of the underlying protective mechanisms, which has limited a uniform and widespread implementation of liver-cooling techniques. This review therefore addresses how hypothermia-induced hypometabolism modulates hepatocyte metabolism during ischemia and thereby reduces hepatic I/R injury. The mechanisms underlying hypothermia-mediated reduction in energy expenditure during ischemia and the attenuation of mitochondrial production of reactive oxygen species during early reperfusion are described. It is further addressed how hypothermia suppresses the sterile hepatic I/R immune response and preserves the metabolic functionality of hepatocytes. Lastly, a summary of the clinical status quo of the use of liver cooling for liver resection and transplantation is provided.

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Section: Nadsupporting

confidence: 53%

Section: Nadsupporting

confidence: 53%

Section: Hypothermia and Glucose Metabolismmentioning

confidence: 97%

Section: Hypothermia and Glucose Metabolismmentioning

confidence: 99%

Section: Hypothermia and Glucose Metabolismmentioning

confidence: 99%

See 3 more Smart Citations

Protective Mechanisms of Hypothermia in Liver Surgery and Transplantation

Olthof

Reiniers

Dirkes

et al. 2015

Mol Med

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Hepatic regeneration and functional recovery following partial liver resection in an experimental model of hepatic steatosis treated with omega-3 fatty acids

Marsman

Graaf

Heger

et al. 2013

British Journal of Surgery

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Chemical probes based on GOLD: The discovery and elucidation of mechanism

Ciaramicoli

Chang

2023

Bulletin Korean Chem Soc

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This review discusses the use of fluorescent probes for identifying, isolating, and monitoring cells and organelles. Fluorescent probes have advantages over antibodies in live‐cell staining due to their small size and ability to penetrate the cellular membrane without fixatives or permeabilizers. However, different cell types exhibit different staining mechanisms based on their genetic profiles and the composition of proteins, enzymes, and lipids. The authors introduce a gating‐oriented live‐cell distinction mechanism where membrane transporters such as solute carriers and ATP are responsible for importing and exporting fluorescent probes. The mechanism for known and unknown transporter identification is elucidated through dose‐dependency, inhibitor tests, and genetic techniques such as clustered regularly interspaced short palindromic repeat modulation and screening. This review provides insights into cellular staining and its potential for understanding the gene profile of different cell types, as well as guiding the identification of membrane transporters.

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Quantitative Assessment of Liver Function after Ischemia-Reperfusion Injury and Partial Hepatectomy in Rats

Cited by 28 publications

References 35 publications

Protective Mechanisms of Hypothermia in Liver Surgery and Transplantation

Protective Mechanisms of Hypothermia in Liver Surgery and Transplantation

Hepatic regeneration and functional recovery following partial liver resection in an experimental model of hepatic steatosis treated with omega-3 fatty acids

Chemical probes based on GOLD: The discovery and elucidation of mechanism

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