Alkaline Phosphatase–Triggered Immunoassay Based on Fluorogenic Reaction for Sensitive Detection of Acetochlor, Metolachlor, and Propisochlor

Zha, Yonghong; Hu, Pan; Lu, Shiying; Ren, Hong-Lin; Liu, Zengshan; Liang, Xiongyan; Yang, Yuying; Li, Yansong; Zhou, Yu

doi:10.1007/s12161-020-01706-6

Cited by 12 publications

(11 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A comparison of the calibration plots is shown in Figure 2. In comparison to previous reports (Table 1), the sensitivity was improved by 10−100 times than ELISA, 26,34−37 100−1000 times than fluoroimmunoassays, 25,38,39 and 10−100 times than lateral flow immunoassays. 40,41 Although the sensitivity of magnetic particle (MNP)-based ELISA also reached 0.05 ng/mL, the preparation process of the MNP−antibody probe is relatively complex and expensive, and the MNP-based ELISA can only be used for metolachlor.…”

Section: ■ Introductioncontrasting

confidence: 66%

“…The standard calibration equations were established by plotting inhibition rates against the logarithm of ATC concentrations. Inhibition rate (%) = (1 – I / I 0 ) × 100%, where I and I 0 represent the fluorescence intensity (au) in the presence and absence of ATC, respectively …”

Section: Materials and Methodsmentioning

confidence: 99%

“…The large specific surface area enables AuNP to carry more FAM-dsDNA to increase the signal strength and IgG to recognize McAb. As a result of probe enrichment, a trace of herbicide is directly associated with numerous DNAs, making this novel immunoassay present higher sensitivity than our previously developed methods for ATC, metolachlor, and propisochlor. , …”

Section: Introductionmentioning

confidence: 99%

“…As a result of probe enrichment, a trace of herbicide is directly associated with numerous DNAs, making this novel immunoassay present higher sensitivity than our previously developed methods for ATC, metolachlor, and propisochlor. 25,26 ■ MATERIALS AND METHODS Reagents and Instruments. All DNA sequences (highperformance liquid chromatography (HPLC) grade) were ordered from Sangon Biotech Co., Ltd. (Shanghai, China) and are displayed in Table S1.…”

Section: ■ Introductionmentioning

confidence: 99%

“…29 The restoring efficiency was counted as follows: restoring efficiency (%) = (F r − F)/F 0 × 100%, where F r is the fluorescence intensity (au) after DSN cleavage. 25 Cross-Reactivity (CR) Study. Selectivity of the proposed "turn-off" fluorescence immunoassay, namely, CR, was evaluated by seven homologous herbicides.…”

Section: ■ Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Duplex-Specific Nuclease-Triggered Fluorescence Immunoassay Based on Dual-Functionalized AuNP for Acetochlor, Metolachlor, and Propisochlor

Zha

et al. 2021

Anal. Chem.

Self Cite

View full text Add to dashboard Cite

Given the great harm of pesticide residues to the environment and public health, exploring ultrasensitive and low-cost methods for their quantitative analysis becomes intensely necessary. Herein, we proposed a double-functionalized gold nanoparticle (AuNP) probe as a signal amplification immunoassay for the detection of acetochlor (ATC), metolachlor, and propisochlor. The AuNP was modified with IgG and fluorophore-labeled duplex DNA by a polyadenine-based freezing method. The quenched fluorescence can be effectively recovered via duplex-specific nuclease (DSN) with excellent cleaving activity. This approach provided limits of detection (LODs) down to 0.03 ng/mL for ATC, 0.10 ng/mL for metolachlor, 0.14 ng/mL for propisochlor, and 0.08 ng/ mL for their mixture. The average recoveries of ATC, metolachlor, and propisochlor were 93.0−106.6% from a corn sample, which are in good agreement with the commercial kit (R 2 = 0.9995). This "turn-off" fluorescence immunoassay presents considerable potential in the analysis of chloroacetamide herbicide due to its simple process of probe preparing and ultrahigh sensitivity.

show abstract

Section: ■ Introductioncontrasting

confidence: 66%

Section: Materials and Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Duplex-Specific Nuclease-Triggered Fluorescence Immunoassay Based on Dual-Functionalized AuNP for Acetochlor, Metolachlor, and Propisochlor

Zha

et al. 2021

Anal. Chem.

Self Cite

View full text Add to dashboard Cite

show abstract

Metolachlor Detection in Grain Using N‐Doped Carbon Quantum Dots and the Intramolecular Charge Transfer Effect

Wang,

Zhang,

2024

Physica Status Solidi (a)

View full text Add to dashboard Cite

Herein, nitrogen is doped into carbon quantum dots (N‐CQDs) using a hydrothermal method for the rapid detection of metolachlor in grain. The morphological features, elemental compositions, and optical properties of the N‐CQDs are then analyzed and investigated using transmission electron microscopy, X‐ray photoelectron spectroscopy, and fluorescence spectroscopy, respectively. Based on the principle of intramolecular charge transfer, a fluorescent probe is constructed for the rapid detection of metolachlor. Under optimal experimental conditions, the fluorescence intensity change values of the N‐CQDs and metolachlor concentration have a good linear relationship when the concentration of metolachlor is in the range of 0.0125−2.5 μg mL−1. An evaluation of the method shows that the method has good selectivity, reproducibility, and stability, with a limit of detection of 1.63 μg kg−1 and a limit of quantification of 3.92 μg kg−1. The spiked recoveries of six real samples are tested using a spiked recovery assay that yielded spiked recoveries in the range of 105.05−87.13%, and their relative standard deviations (n = 3) ranged from 4.62% to 0.61%, indicating that the method can be used for detection in actual samples with good precision and stability.

show abstract

Assays for alkaline phosphatase that use L-ascorbic acid 2-phosphate as a substrate

Wu,

Chen,

Wang

et al. 2023

Coordination Chemistry Reviews

View full text Add to dashboard Cite

Alkaline Phosphatase–Triggered Immunoassay Based on Fluorogenic Reaction for Sensitive Detection of Acetochlor, Metolachlor, and Propisochlor

Cited by 12 publications

References 56 publications

Duplex-Specific Nuclease-Triggered Fluorescence Immunoassay Based on Dual-Functionalized AuNP for Acetochlor, Metolachlor, and Propisochlor

Duplex-Specific Nuclease-Triggered Fluorescence Immunoassay Based on Dual-Functionalized AuNP for Acetochlor, Metolachlor, and Propisochlor

Metolachlor Detection in Grain Using N‐Doped Carbon Quantum Dots and the Intramolecular Charge Transfer Effect

Assays for alkaline phosphatase that use L-ascorbic acid 2-phosphate as a substrate

Contact Info

Product

Resources

About