Alkaloid production by immobilized mycelia of Claviceps purpurea

Kopp, Bettina; Rehm, H. J.

doi:10.1007/bf00500489

Cited by 34 publications

(4 citation statements)

References 15 publications

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“…Immobilization in alginates of medium and low viscosity enabled a long semicontinuous fermentation. The 2%-4% concentrations of alginate did not seem to affect the changes in the alkaloid spectrum, as found also for C. fusiformis cells (Kren et al 1987), but not for C. purpurea cells (Kopp and Rehm 1983). The results indicated that it was better to immobilize C. paspali mycelium during the growth phase.…”

Section: Discussionsupporting

confidence: 51%

Production of lysergic acid derivatives with immobilized Claviceps paspali mycelium

Rozman

Pertot

Komel

et al. 1989

Appl Microbiol Biotechnol

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A semicontinuous fermentation with immobilized mycelium of Claviceps paspali, producing mainly methylcarbinolamide of lysergic acid, ergometrine and the amide of lysergic acid, was carried out. It was found that immobilization itself improved ergometrine production. The type and concentration of alginate used dit not influence the alkaloid spectrum. The age and quantity of inoculum as well as the length of a particular cycle affected total alkaloid production but did not seem to affect the changes in relative amounts of particular alkaloids. The best results were obtained with 1000 mg dry mycelium weight of 6-day-old fungus from the vegetative phase in 100 ml production medium immobilized in 4% alginate of medium or low viscosity and by recycling every 10 days. When no fungus was found outside the gel beads it was possible to improve the yield of ergometrine from 52% in the first cycle to 85% in the fourth cycle of the semicontinuous procedure.

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Section: Discussionsupporting

confidence: 51%

Production of lysergic acid derivatives with immobilized Claviceps paspali mycelium

Rozman

Pertot

Komel

et al. 1989

Appl Microbiol Biotechnol

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“…Therefore, the suitability of pore size, bead stability and optimum oxygen diffusion were achieved at this concentration. However, concentrations below 3·0% showed low stability and higher concentrations showed reduced diffusion rate affecting activity of entrapped cells (Kopp and Rehm ; Sallam et al . ).…”

Section: Discussionmentioning

confidence: 99%

Immobilization technique for enhanced production of the immunosuppressant mycophenolic acid by ultraviolet and gamma-irradiated Penicillium roqueforti

2015

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“…ology of entrapped cells. Therefore, mechanical hindrance was discussed as an important factor for alkaloid formation by C. purpurea (8). Moreover, the calcium ions may directly affect the immobilized organism (4,20) or decrease the effective phosphate concentration by precipitation (3).…”

Section: Discussionmentioning

confidence: 99%

Long-Term Production of Ergot Peptides by Immobilized Claviceps purpurea in Semicontinuous and Continuous Culture

Dierkes

Lohmeyer

Rehm

1993

Appl Environ Microbiol

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The semicontinuous and continuous production of pharmaceutically useful ergot peptides with immobilized Clavicepspurpurea could be demonstrated. A key aspect was the presence of high concentrations of CaCl2 (96.9 mM) to give marked prolongation of the productive phase, and cultivation in a bubble column reactor became possible. Restriction of the phosphate supply avoided an otherwise problematic massive increase of outgrowing hyphae.

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Alkaloid production by immobilized mycelia of Claviceps purpurea

Cited by 34 publications

References 15 publications

Production of lysergic acid derivatives with immobilized Claviceps paspali mycelium

Production of lysergic acid derivatives with immobilized Claviceps paspali mycelium

Immobilization technique for enhanced production of the immunosuppressant mycophenolic acid by ultraviolet and gamma-irradiated Penicillium roqueforti

Long-Term Production of Ergot Peptides by Immobilized Claviceps purpurea in Semicontinuous and Continuous Culture

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