Alkaloid production in <i>Vernonia cinerea:</i> Callus, cell suspension and root cultures

Maheshwari, Priti; Songara, Bharti; Kumar, Shailesh; Jain, Promil; Srivastava, Kirti; Kumar, Anil

doi:10.1002/biot.200700033

Cited by 15 publications

(14 citation statements)

References 21 publications

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“…2a) as it yields a high contents of secondary metabolites during differentiation (Benavides and Caso, 1993;Maheshwari et al, 2007). The callus retains the capacity to synthesize alkaloid production (Yamamoto and Yamada, 1986;Prem et al, 2002;Anitha and Kumari, 2006).…”

Section: Introductionmentioning

confidence: 99%

In vitro callus induction in inflorescence segments of medicinally important endangered plant Rauwolfia serpentina (L.) Benth. ex Kurz – a step towards ex situ conservation

Kaur¹

2018

Ann.Pl.Sci.

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The regenerative competence of inflorescence segments of Rauwolfia serpentina was assessed in M (Mitra et al., 1976) medium and its combinations with growth adjuncts such as cytokinins [N 6 -benzyladenine (6-BA -0.5, 1.0 mg l -1 ), furfurylaminopurine (Kn-0.5, 1.0mg l -1 ) and auxin [α-naphthalene acetic acid (NAA -0.5, 1.0 mg l -1 )]. Inflorescence segments (1.00 cm in length) were used as explants. The regeneration response was obligatory to the use of growth regulators in the medium. Cytokinins favoured shoot bud mediated pathway of plantlet development and BAP favoured early shooting. The explants callused in NAA enriched medium. The callus was best maintained in NAA (0.5 mg l -1 ) through repeated sub-culturing. The entire plant of R. serpentina is the target of massive commercial collections resulting into shrinking habitats. Present study is the first ever report on the successful use of inflorescence segments for ex situ conservation of R. serpentina.

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Section: Introductionmentioning

confidence: 99%

In vitro callus induction in inflorescence segments of medicinally important endangered plant Rauwolfia serpentina (L.) Benth. ex Kurz – a step towards ex situ conservation

Kaur¹

2018

Ann.Pl.Sci.

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“…Callus culture can facilitate optimization of alkaloids production [2] and subsequent isolation [7]. Tissue grown as callus masses can some times yield high amount of secondary metabolites [8]. The present study was undertaken to study the comparative callus induction potential of various plant parts of Tridax by using plant growth hormones.…”

Section: Introductionmentioning

confidence: 99%

Callus induction studies in Tridax procumbens L.

Wani¹,

Pande²,

More³

2010

Int J of Biot Appl

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Abstract-Tridax procumbens is a common weed plant belonging to the family Asteraceae. The leaf juice has antiseptic, insecticidal and parasiticidal properties. The present work is based on developing a protocol for the callus induction in Tridax procumbens from various explants like leaf, internodes and shoot apical buds. The sterilized explants were inoculated in MS media containing various combination of auxins such as 2, 4, dichlorophenoxy acetic acid (2, 4-D) and naphthalene acetic acid (NAA) and cytokinins such as kinetin and 6 benzyl amino purine (BAP). Leaf and apical bud explants showed early and profuse callus induction whereas internodal explants showed comparatively delayed but profuse callus induction. Leaf and apical bud explants showed maximum response in terms of callus by using MS media with the combination 2, 4-D 0.5mg/lit and BAP 0.5mg/lit which was followed by 2, 4-D 0.5mg/lit and KIN 0.5mg/lit, 2, 4 -D 0.5mg/lit and BAP+KIN 0.5 mg/lit, NAA 0.5mg/lit and BAP 0.5mg/lit, NAA 2mg/lit and BAP 0.5mg/lit respectively. Whereas internodal explants showed maximum callus induction by using a hormonal concentration of 2mg/lit 2,4, D and 0.5mg/lit BAP. In vitro generated callus can be used as a source for the isolation of secondary metabolites from Tridax plant.

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“…Extracts of grown suspended cells (Table 8, Fig 3) showed that high colchicine content of 0.012 mg g -1 DW was obtained at the start of culture after the first week during the lag phase. In Vernonia cinerea cell suspension growth and alkaloid contents were maximal in 20-day-old cultures and alkaloid contents were 1.15 mg/g [30]. Gopi and Vatsala [8] recorded that the gymnemic acids and gymnemagenin were present in cultured cells in Gymnema sylvestre.…”

Section: Resultsmentioning

confidence: 99%

A New Paradigm for Personalized Medicine and Companion Diagnostics: The Contract Diagnostics Organization

Cotter¹

2012

TOPROCJ

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Abstract:Callus was induced from seeds of Colchicum hierosolymitanum Feib inoculated on the surface of MS media supplemented with 0.45 µM 2, 4-dichlorophenoxyacetic acid under dark conditions. Then callus was cultured on MS media supplemented with 4.52 µM 2, 4-dichlorophenoxyacetic for growth and maintenance. Friable callus from the fourth generation was transferred to liquid MS media supplemented with 0.54 µM 1-naphthaleneacetic acid to form cell suspension. Cells were successfully subcultured every 27 days on the same liquid media supplemented with 0.54 µM 1-naphthaleneacetic acid. Higher concentration (9 µM) of 6-benzyladenine with 0.45 µM 2, 4-dichlorophenoxyacetic acid resulted in higher cells fresh weight, while 1-naphthaleneacetic acid combinations with 6-benzyladenine had no effect on cell growth. On the other hand, the time for subculturing the cells into new fresh liquid media was determined to be after 27 days of incubation. (-) -Colchicine was identified in callus and cell suspension of C. hierosolymitanum by performing HPLC analysis against standard. Different ratios of NH +4 : NO -3 were used to study their effect on (-)-colchicine content, the highest colchicine content of 0.070 mg g -1 DW was obtained at 30 mM NH 4 + of total nitrogen. Colchicine alkaloid was highest, 0.090 mg g -1 DW, at 0.1 M of sucrose after 4 weeks incubation. (-) -Colchicine alkaloid was not detected in callus grown on sucrose free media. Maximum production of colchicine, 0.235 mg g -1 DW, was obtained in callus extracts of 60 days old callus grown under dark conditions. Cell suspension had 0.012 mg g-1 DW (-) -colchicine from suspended cells grown under dark. (-) -Colchicine content of callus incubated under dark (0.095 mg g -1 DW) was higher than light (0.070 mg g -1 DW) condition.

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Alkaloid production in Vernonia cinerea: Callus, cell suspension and root cultures

Cited by 15 publications

References 21 publications

In vitro callus induction in inflorescence segments of medicinally important endangered plant Rauwolfia serpentina (L.) Benth. ex Kurz – a step towards ex situ conservation

In vitro callus induction in inflorescence segments of medicinally important endangered plant Rauwolfia serpentina (L.) Benth. ex Kurz – a step towards ex situ conservation

Callus induction studies in Tridax procumbens L.

A New Paradigm for Personalized Medicine and Companion Diagnostics: The Contract Diagnostics Organization

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