Callus induction studies in Tridax procumbens L.

Wani, Minal; Pande, Snehal; More, N.V. More N.V.

doi:10.9735/0975-2943.2.1.11-14

Cited by 21 publications

(6 citation statements)

References 10 publications

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“…Moreover, Taha et al [20] established promising protocol for callus production from Helianthus Tuberosus nodal stem and leaf explants by augmentation of MS-medium with 1 mg/l each of NAA and BAP. Further, Wani et al [21] reported that, leaf and apical bud explants of Tridax procumbens showed maximum response in terms of callus using MS medium with the combination of 2,4-D (0.5 mg/l) and BAP (0.5mg/l).…”

Section: Callus Induction and Shoots Regenerationmentioning

confidence: 99%

New Aspects for Callus Production, Regeneration and Molecular Characterization of Ginger (Zingiber officinale Rosc.)

Taha¹

2013

Med Aromat Plants

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Callus induction and shoots regeneration Callus induction: The multiplication in vitro plantlets were obtained previously as described by Abbas et al. [11]. Callus cultures were initiated from in vitro primordial leaves of 8-weeks-old plantlets cultures. The basal primordial leaves were cut to small segments (0.5-1.0 cm) nearly uniform in size and placed in glass jars (50 ml) containing MS [12 ] solid medium, 3% sucrose and 0.7% agar supplemented with various concentrations of 2,4-D and BAP as follow: CB MS-Free growth regulators C1 MS+2 mg/l 2,4-D C2 MS+3 mg/l 2,4-D C3 MS+4 mg/l 2,4-D C4 MS+2 mg/l 2,4-D+0.2 mg/l BAP C5 MS+3 mg/l 2,4-D+0.2 mg/l BAP C6 MS+4 mg/l 2,4-D+0.2 mg/l BAP Cultures of all treatments were divided to two groups. The first group incubated and maintained under 16-h photoperiod at intensity of 3000 Lux from cool white light of fluorescent lamps (Phillips). The second group maintained under darkness condition. All cultures

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Section: Callus Induction and Shoots Regenerationmentioning

confidence: 99%

New Aspects for Callus Production, Regeneration and Molecular Characterization of Ginger (Zingiber officinale Rosc.)

Taha¹

2013

Med Aromat Plants

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“…The interaction effect of 2,4-D and BAP on callus induction of other medicinal plants were reported on Tridax procumbens [34], Amsonia orientalis [35], Swertia chirayita [36], Strobilanthus foliosus [37], Stevia rebaudiana [38], Scrophularia striata [39].…”

Section: Callus Inductionmentioning

confidence: 99%

In Vitro Callus Induction and Shoot Regeneration from Leaf Explants of Glinus lotoides (L.)—An Important Medicinal Plant

Teshome¹,

Feyissa²

2015

AJPS

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G. lotoides L. is a threatened plant that is frequently harvested for medicinal purpose. However, its distribution in the world is limited because of short period of seed viability and poor seed germination. The objective of this study was to develop in vitro propagation protocol for G. lotoides through callus induction. For callus induction, different concentrations of 2,4-D (2,4-dichlorophenoxyacetic acid), NAA (α-naphthalene acetic acid) and BAP (6-benzyl amino purine) were used. Seeds were sown on growth regulator-free MS medium and shoots from the in vitro germinated seedlings were excised and cultured on MS medium containing 0.5 mg/l BAP. Young leaves from these shoots were used as explant for callus induction and shoot regeneration. Maximum callus induction (100%) was observed on medium containing 2,4-D (0.5, 2.0, 3.5 mg/l) or NAA (2.0, 2.5 mg/l) in combination with 0.5 mg/l BAP. However, 2,4-D was the best in overall callus induction. The highest regeneration (20%) frequency was achieved on the medium containing 0.5 mg/l BAP. Highest number of shoot (2.83 ± 1.22) and shoot length (2.16 ± 0.87 cm) per explant were obtained in the presence of 0.25 mg/l BAP + 0.5 mg/l KIN (Kinetin). In shoot multiplication media, maximum mean (6.43 ± 0.87) of shoot was observed on MS medium containing 0.5 mg/l BAP. The best shoot length (1.70 ± 0.14 cm) was recorded on control medium. The highest (95%), maximum root number (14.10 ± 1.47) and root length (1.01 ± 0.10 cm) were obtained on a medium supplemented with 1.5 mg/l Indole-3-butyric acid (IBA). All the plants (100%) were survived after acclimatization in greenhouse. The present study can be useful for callus induction and indirect shoot regeneration form G. lotoides.

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“…Many plant tissue culture were showed the positive response to callus induction by 2,4 dichloro phenoxy acetic acid (2,4 D) as auxin source i.e. callus of Tridax procumbens (Wani, et al, 2010) or Jatropha curcas (Antonio, et al, 2014). Some other species, Hibiscus sabdariffa (Nurchayati & Rahmah, 2010;Noviati et al, 2014); and Catharanthus roseus responded to NAA instead of 2,4 D (Darsini, 2011) to induce callogenesis.…”

Section: Callus Growth In Ms Mediummentioning

confidence: 99%

Growth Pattern and Copper Accumulation in Callus of Datura metel

et al. 2016

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This experiment was aimed to evaluate the copper accumulation using callus culture of Datura metel L. The culture was established from leaves onto MS contained NAA 2.5 mg/L and Kinetin 0.5 mg/L as the control. The exposure of the culture was carried out by 2 copper compounds as treatment, i.e. CuCl2.2H2O and Na2CuEDTA at level concentration 0.; 0.1; 5; 10; 15; and 20 µM. The growth pattern of callus in control showed increasing growth rate in 36 days, whereas exponential stage was reached at 12-20th doi*. Whilst, after 10 doi, the treatment showed constant growth pattern. The absorption rate of the culture was increased by the addition of the CuCl2.2H2O at 5 – 15 µM of level concentration but declined at 20µM. The maximum rate of accumulation of Cu (0,1519 mg g-1) was obtained at 15 µM. Instead, the addition of Na2CuEDTA at 5 – 20µM of level concentration showed the significant increment while the maximum accumulation was obtained at 20µM (0,1420 mg g-1). The existence of chelator in copper compound reduced the rate of toxicity while all tolerance index values were between 66,24 and 97,28 %.The results suggested the role of callus of D. metel as that fairly absorbed and accumulated Cu2+. Exposure with CuCl2.2H2O indicated higher accumulation than Na2CuEDTA.How to CiteNurchayati, Y., Santosa, S., Nugroho, L. H., & Indrianto, A. (2016). Growth Pattern and Copper Accumulation in Callus of Datura metel. Biosaintifika: Journal of Biology & Biology Education, 8(2), 135-140.

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Callus induction studies in Tridax procumbens L.

Cited by 21 publications

References 10 publications

New Aspects for Callus Production, Regeneration and Molecular Characterization of Ginger (Zingiber officinale Rosc.)

New Aspects for Callus Production, Regeneration and Molecular Characterization of Ginger (Zingiber officinale Rosc.)

<i>In Vitro</i> Callus Induction and Shoot Regeneration from Leaf Explants of <i>Glinus lotoides</i> (L.)—An Important Medicinal Plant

Growth Pattern and Copper Accumulation in Callus of Datura metel

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Callus induction studies in Tridax procumbens L.

Cited by 21 publications

References 10 publications

New Aspects for Callus Production, Regeneration and Molecular Characterization of Ginger (Zingiber officinale Rosc.)

New Aspects for Callus Production, Regeneration and Molecular Characterization of Ginger (Zingiber officinale Rosc.)

&lt;i&gt;In Vitro&lt;/i&gt; Callus Induction and Shoot Regeneration from Leaf Explants of &lt;i&gt;Glinus lotoides&lt;/i&gt; (L.)—An Important Medicinal Plant

Growth Pattern and Copper Accumulation in Callus of Datura metel

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<i>In Vitro</i> Callus Induction and Shoot Regeneration from Leaf Explants of <i>Glinus lotoides</i> (L.)—An Important Medicinal Plant