Alkaloid transporters in plants

Shitan, Nobukazu; Kato, Keita; Shoji, Tsubasa

doi:10.5511/plantbiotechnology.14.1002a

Cited by 32 publications

(26 citation statements)

References 76 publications

(106 reference statements)

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“…Recently, it was revealed that a member of the NPF transporter family was related to strictosidine transport and other members of the NPF family have been implicated in iridoid glucoside transport (Larsen et al, 2017;Payne et al, 2017). It has also been reported that various transporters may be involved in the transport of alkaloids (Carqueijeiro et al, 2013;Shitan et al, 2014). Further studies are needed to clarify how these compounds distribute between the different cell types in C. roseus, and how the site of biosynthesis may differ from the site of accumulation.…”

Section: Cell-specific Localisation Of Tias In Leaf Tissuementioning

confidence: 99%

The complexity of intercellular localisation of alkaloids revealed by single‐cell metabolomics

et al. 2019

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Summary Catharanthus roseus is a medicinal plant well known for producing bioactive compounds such as vinblastine and vincristine, which are classified as terpenoid indole alkaloids (TIAs). Although the leaves of this plant are the main source of these antitumour drugs, much remains unknown on how TIAs are biosynthesised from a central precursor, strictosidine, to various TIAs in planta. Here, we have succeeded in showing, for the first time in leaf tissue of C. roseus, cell‐specific TIAs localisation and accumulation with 10 μm spatial resolution Imaging mass spectrometry (Imaging MS) and live single‐cell mass spectrometry (single‐cell MS). These metabolomic studies revealed that most TIA precursors (iridoids) are localised in the epidermal cells, but major TIAs including serpentine and vindoline are localised instead in idioblast cells. Interestingly, the central TIA intermediate strictosidine also accumulates in both epidermal and idioblast cells of C. roseus. Moreover, we also found that vindoline accumulation increases in laticifer cells as the leaf expands. These discoveries highlight the complexity of intercellular localisation in plant specialised metabolism.

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Section: Cell-specific Localisation Of Tias In Leaf Tissuementioning

confidence: 99%

The complexity of intercellular localisation of alkaloids revealed by single‐cell metabolomics

et al. 2019

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“…37) For further detailed information about alkaloid transport, please refer to previous reviews. 45,46) I.ii. Phenolic compounds Phenolic compounds have at least one aromatic ring with one or more hydroxyl substituents, and consist of several subgroups, e.g.…”

Section: II Alkaloidsmentioning

confidence: 99%

Secondary metabolites in plants: transport and self-tolerance mechanisms

Shitan

2016

Bioscience, Biotechnology, and Biochemistry

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Plants produce a host of secondary metabolites with a wide range of biological activities, including potential toxicity to eukaryotic cells. Plants generally manage these compounds by transport to the apoplast or specific organelles such as the vacuole, or other self-tolerance mechanisms. For efficient production of such bioactive compounds in plants or microbes, transport and self-tolerance mechanisms should function cooperatively with the corresponding biosynthetic enzymes. Intensive studies have identified and characterized the proteins responsible for transport and self-tolerance. In particular, many transporters have been isolated and their physiological functions have been proposed. This review describes recent progress in studies of transport and self-tolerance and provides an updated inventory of transporters according to their substrates. Application of such knowledge to synthetic biology might enable efficient production of valuable secondary metabolites in the future.

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“…To date, the plant alkaloid transporters are mainly characterized into the ATP-binding cassette (ABC) protein, multidrug and toxic compound extrusion (MATE), and purine permease (PUP) families. Some transporters were found to be required for the efficient biosynthesis of alkaloids in plants [ 45 ]. In tobacco, several alkaloid transporter genes have been identified, such as tobacco jasmonate-inducible alkaloid tranporter1 ( Nt - JAT1 ), Nt - JAT2 , tobacco nicotine uptake permease1 ( Nt - NUP1 ), NtMATE1 and NtMATE2 [ 46 – 50 ].…”

Section: Resultsmentioning

confidence: 99%

Sequencing and characterization of leaf transcriptomes of six diploid Nicotiana species

Long

Ren

Xiang

et al. 2016

J of Biol Res-Thessaloniki

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BackgroundNicotiana belongs to the Solanaceae family that includes important crops such as tomato, potato, eggplant, and pepper. Nicotiana species are of worldwide economic importance and are important model plants for scientific research. Here we present the comparative analysis of the transcriptomes of six wild diploid Nicotiana species. Wild relatives provide an excellent study system for the analysis of the genetic basis for various traits, especially disease resistance.ResultsWhole transcriptome sequencing (RNA-seq) was performed for leaves of six diploid Nicotiana species, i.e. Nicotiana glauca, Nicotiana noctiflora, Nicotiana cordifolia, Nicotiana knightiana, Nicotiana setchellii and Nicotiana tomentosiformis. For each species, 9.0–22.3 Gb high-quality clean data were generated, and 67,073–182,046 transcripts were assembled with lengths greater than 100 bp. Over 90 % of the ORFs in each species had significant similarity with proteins in the NCBI non-redundant protein sequence (NR) database. A total of 2491 homologs were identified and used to construct a phylogenetic tree from the respective transcriptomes in Nicotiana. Bioinformatic analysis identified resistance gene analogs, major transcription factor families, and alkaloid transporter genes linked to plant defense.ConclusionsThis is the first report on the leaf transcriptomes of six wild Nicotiana species by Illumina paired-end sequencing and de novo assembly without a reference genome. These sequence resources hopefully will provide an opportunity for identifying genes involved in plant defense and several important quality traits in wild Nicotiana and will accelerate functional genomic studies and genetic improvement efforts of Nicotiana or other important Solanaceae crops in the future.Electronic supplementary materialThe online version of this article (doi:10.1186/s40709-016-0048-5) contains supplementary material, which is available to authorized users.

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Alkaloid transporters in plants

Cited by 32 publications

References 76 publications

The complexity of intercellular localisation of alkaloids revealed by single‐cell metabolomics

The complexity of intercellular localisation of alkaloids revealed by single‐cell metabolomics

Secondary metabolites in plants: transport and self-tolerance mechanisms

Sequencing and characterization of leaf transcriptomes of six diploid Nicotiana species

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