Alkaloids of Phaedranassa dubia (Kunth) J.F. Macbr. and Phaedranassa brevifolia Meerow (Amaryllidaceae) from Ecuador and its cholinesterase-inhibitory activity

Abstract

Highlight The role of Amaryllidaceae species from Ecuador as source of new drugs. High amount of lycorine-type alkaloids in Phaedranassa dubia by GC-MS. In vitro potential of Phaedranassa brevifolia as cholinesterase inhibitor. Theoretical inhibitory activity of alkaloids against cholinesterase enzymes.

Pharmacology and Phytochemistry of Ecuadorian Medicinal Plants: An Update and Perspectives

“…The anti-inflammatory and cytotoxic activities were evaluated using isolated neutrophils and the tetrazolium salt method (WST-1), respectively [16]. The alkaloid profile of each species was analyzed using GC/MS [17,18]; a high content of lycorine-type alkaloids was found in P. dubia and P. brevifolia bulbs [18].…”

“…However, this compound was not present in the extracts of the two plants that exhibited the highest in vitro inhibitory activity, namely, P. cuencana (IC 50 = 0.88 ± 0.11 µg/mL) against AChE and P. dubia (IC 50 = 14.26 ± 2.71 µg/mL) against BuChE; instead, the two species were rich in galanthamine-type alkaloids [17]. The alkaloid profiles of P. dubia and P. brevifolia bulbs were investigated and significant inhibitory activities on both enzymes were determined [18]. The IC 50 values of the activity against AChE were 25.48 ± 0.39 and 3.45 ± 0.29 µg/mL, respectively, whereas the IC 50 values against BuChE were 96 ± 4.…”

“…94 and 58.89 ± 0.55 µg/mL, respectively. A high content of lycorine-type alkaloids was found in both species [18]. The plant showed no significant antioxidant and antiradical effects [20].…”

“…The exudate latex of the stem bark is used to eliminate warts and to treat wounds, sores and ulcers (3R,20S)-3,20-dihydroxydammara-24-ene 3-O-palmitate ( 17), (3R,20S)-3,20-dihydroxydam-mara-24-ene 3-O-acetate (18), trans-phytol (19), vomifoliol (20), β-sitosterol (21), trans-tiliroside (22), and sparsifol (23) (Figure 4) were isolated from the plant [53].…”

Structural Diversity and Biological Potential of Alkaloids from the Genus Hippeastrum, Amaryllidaceae: an Update

“…The 5,10b-ethanophenanthridine skeleton of alkaloids belonging to haemanthamine- and crinine-type usually is confirmed by nuclear magnetic resonance (NMR) and circular dichroism (CD) (León et al 2021 ). Among the Hippeastrum studies covered in this review, some alkaloids from the haemanthamine group were only identified by GC–MS, which lead to their assignment as isomers, such as occurs with vittatine shown in Table S1 (Ortiz et al 2016 ; Shammari et al 2019 ; Lianza et al 2020 ; Souza et al 2021 ).…”