Allele frequency data of 21 autosomal short tandem repeat loci in Mesan and Basra provinces in South Iraq

Hameed, Imad Hadi; Ommer, Aamera Jaber; Murad, Abeer Fauzi; Mohammed, Ghaidaa Jehadi

doi:10.1016/j.ejfs.2014.10.003

Cited by 15 publications

(10 citation statements)

References 15 publications

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“…Allele frequency from allele sharing in this study were 247 FES, F13, D8S1179, CSF1PO, D16S539, D7S820, and 248 THO1, respectively, 100%, 100%, 92%, 92%, 88%, 88%, 249 and 84% (Table 2). This result is slightly different from the findings of Hameed et al (2015), D16S539, THO1, vWA, D5S818, D8S1179, D3S1357, CSF1PO, respectively, 88%, 81%, 80%, 77%, 74%, 72%, and 71%. From the sequence to 7 loci from the 2 studies there are 4 similar loci that can be used as references, namely, D8S1179, CSF1PO, D16S539, and THO1.…”

Section: Resultscontrasting

confidence: 86%

Full-sibling allelic frequency and sharing among Madurese: STR technique by 12 locus and the sex-typing amelogenin gene

Sosiawan

Yudianto

Furqoni

et al. 2019

Egypt J Forensic Sci

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Background: Sibling relation over chosen STR loci and localized database is yet to be developed in Indonesia like into many other countries despite the demand of varied ethnic population, catastrophes and civil conflict that call forensic preparedness. The targeted application includes human genomic typing and profile, kinship/sibship relation, forensic applications, individual identification, and or claneology tracing. Consequently, this study devotes on sibship in assessing and establishing preferable STR loci and allelic sharing extent amongst Madurese full sibling. Materials and methods: One hundred blood samples were used to examine sibling relationship among 50 full siblings (25 pairs) assigned from a total of 100 individuals of 25 Madura families. Sibship relation was done by STR technique profile by 12 loci (CSF1PO, F13B, FES, TH01, TPOX, vWA, D5S818, D7S820, D8S1179, D13S317, D16S539, and D18S51) and a sex-typing amelogenin gene locus Results: From 300 observations (25 pairs of full-sibling × 12 loci) of genotyped STR allele, of allele sharing, has found 4 STR loci: FES, CSF1PO, F13, and D8S1179 with high-shared allelic frequency in two alleles sharing among twelve STR loci by 52%, 52%, 44%, and 40% respectively. As for the four main loci of full sibling with one allele sharing, i.e, D7S820, D18S51, vWA, and THO1, have a sequence of percentages from large to small as follows: 76%, 76%, 72%, and 60%, respectively. Furthermore, STR locus VWA expressed null shared allele in 24 allele-sharing category. Likewise, F13 and FES both typed null allele in zero allele-sharing category among these full sibling Conclusion: Through the use of 12 autosomal STR loci, the study has signified the strength of 2 shared allele evidence by 51.2% in establishing sibship and recommend D7S820, VWA, TH01, and D18S51 as STR loci of choice when typing sibling among Madurese.

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Section: Resultscontrasting

confidence: 86%

Full-sibling allelic frequency and sharing among Madurese: STR technique by 12 locus and the sex-typing amelogenin gene

Sosiawan

Yudianto

Furqoni

et al. 2019

Egypt J Forensic Sci

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“…Holland et al (2011) showed that the polymorphism of mtDNA coding area is less than that of mtDNA control region. Therefore, more efficient poly-morphic sites should be used to provide an improved discrimination power for forensic mtDNA testing (Nadia et al, 2011;Imad et al, 2015c;Muhanned et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

Analysis of mtDNA hypervariable region II for increasing the discrimination power from Middle and South of Iraq

Ameera¹,

Muhanned²,

Imad³

2015

Afr. J. Biotechnol.

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Mitochondrial DNA is a useful genetic marker for answering evolutionary questions due to its high copy number, maternal mode of inheritance, and its high rate of evolution. The aims of this research were to study the mitochondria noncoding region by using the sanger sequencing technique and establish the degree of variation characteristic of a fragment FTA® Technology (FTA™ paper DNA extraction) utilized to extract DNA. A portion of a non-coding region encompassing positions 37 to 340 for HVII was amplified in accordance with the Anderson reference sequence. PCR products were purified by EZ-10 spin column then sequenced and detected by using the ABI 3730xL DNA analyzer. New polymorphic positions G92C, C113G, C150G, T156A, C194G, C198G, G207C, G225C and G228C are described and may in future be suitable sources for identification purpose. The data obtained can be used to identify variable nucleotide positions characterized by frequent occurrence most promising for identification variants.

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“…With this caveat in mind, we combined genotypes across years for our remaining analyses. To assess the quality of our markers, we calculated the number of alleles per locus, the observed and expected heterozygosity, polymorphism information content, and random match probability for each locus ([44]; S1 Table). We use the last two metrics as a proxy for the reliability of our dataset for distinguishing population-level patterns of differentiation.…”

Section: Methodsmentioning

confidence: 99%

Historical Environment Is Reflected in Modern Population Genetics and Biogeography of an Island Endemic Lizard (Xantusia riversiana reticulata)

2016

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The restricted distribution and isolation of island endemics often produces unique genetic and phenotypic diversity of conservation interest to management agencies. However, these isolated species, especially those with sensitive life history traits, are at high risk for the adverse effects of genetic drift and habitat degradation by non-native wildlife. Here, we study the population genetic diversity, structure, and stability of a classic “island giant” (Xantusia riversiana, the Island Night Lizard) on San Clemente Island, California following the removal of feral goats. Using DNA microsatellites, we found that this population is reasonably genetically robust despite historical grazing, with similar effective population sizes and genetic diversity metrics across all sampling locations irrespective of habitat type and degree of degradation. However, we also found strong site-specific patterns of genetic variation and low genetic diversity compared to mainland congeners, warranting continued special management as an island endemic. We identify both high and low elevation areas that remain valuable repositories of genetic diversity and provide a case study for other low-dispersal coastal organisms in the face of future climate change.

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Allele frequency data of 21 autosomal short tandem repeat loci in Mesan and Basra provinces in South Iraq

Cited by 15 publications

References 15 publications

Full-sibling allelic frequency and sharing among Madurese: STR technique by 12 locus and the sex-typing amelogenin gene

Full-sibling allelic frequency and sharing among Madurese: STR technique by 12 locus and the sex-typing amelogenin gene

Analysis of mtDNA hypervariable region II for increasing the discrimination power from Middle and South of Iraq

Historical Environment Is Reflected in Modern Population Genetics and Biogeography of an Island Endemic Lizard (Xantusia riversiana reticulata)

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