Allele-specific polymerase chain reaction for the detection of single nucleotide polymorphism in amlodipine-induced gingival enlargement

Kala, Nitya; Babu, S P K Kennedy; Manjeu, J.; Aadivalavan, A.; Khan, Rajwali

doi:10.1111/jcpt.12587

Cited by 6 publications

(4 citation statements)

References 10 publications

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“…In AS-PCR, Taq polymerase can effectively carry out DNA synthesis reactions only when the base at the 3′ end of the primer is correctly matched with the template DNA [ 61 , 69 ]. The primers were designed based on the SNP of TraesCS7B01G164000 detected in wheat L658 and L958, as shown in Table S4 .…”

Section: Methodsmentioning

confidence: 99%

Identification and Cloning of a CC-NBS-NBS-LRR Gene as a Candidate of Pm40 by Integrated Analysis of Both the Available Transcriptional Data and Published Linkage Mapping

Yang

Zhong

Chen

et al. 2021

IJMS

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Wheat powdery mildew, caused by the obligate parasite Blumeria graminis f. sp. tritici, severely reduces wheat yields. Identifying durable and effective genes against wheat powdery mildew and further transferring them into wheat cultivars is important for finally controlling this disease in wheat production. Pm40 has been widely used in wheat breeding programs in Southwest China due to the spectrum and potentially durable resistance to powdery mildew. In the present study, a resistance test demonstrated that Pm40 is still effective against the Bgt race E20. We identified and cloned the TraesCS7B01G164000 with a total length of 4883 bp, including three exons and two introns, and encoded a protein carrying the CC-NBS-NBS-LRR domain in the Pm40-linked region flanked by two EST markers, BF478514 and BF291338, by integrating analysis of gene annotation in wheat reference genome and both sequence and expression difference in available transcriptome data. Two missense mutations were detected at positions 68 and 83 in the CC domain. The results of both cosegregation linkage analysis and qRT-PCR also suggested that TraesCS7B01G164000 was a potential candidate gene of Pm40. This study allowed us to move toward the final successfully clone and apply Pm40 in wheat resistance improvement by gene engineering.

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Section: Methodsmentioning

confidence: 99%

Identification and Cloning of a CC-NBS-NBS-LRR Gene as a Candidate of Pm40 by Integrated Analysis of Both the Available Transcriptional Data and Published Linkage Mapping

Yang

Zhong

Chen

et al. 2021

IJMS

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“…Clínicamente, se observa un crecimiento anormal de la encía, en casos muy graves puede llegar a cubrir todas las piezas dentales, en casos menores se observa papilas interdentales en forma de una superficie lobulada y fibrótica de color rosado. Los fármacos antihipertensivos relacionados son: los bloqueadores de los canales de calcio y calcio antagonista (J et al, 2021;Kala et al, 2018;Kose et al, 2020;Lauritano et al, 2019;Vidal et al, 2018)…”

Section: Hiperplasia Gingivalunclassified

Medicación antihipertensiva y sus reacciones adversas en la cavidad oral. Una revisión integrativa

Armijos

Espin

Carrera

2022

RSD

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El uso de la medicación antihipertensiva ha permitido reducir las complicaciones relacionadas con la hipertensión arterial. Sin embargo, el uso de estos fármacos ha generado reacciones adversas en la cavidad bucal como la xerostomía, hiperplasia gingival, alteraciones del gusto, entre otras. El presente estudio tiene como objetivo identificar en la literatura las reacciones adversas o efectos indeseables relacionadas a la terapia antihipertensiva. Se realizó una búsqueda en las bases digitales en PubMed, Scopus, Scielo y Google Académico, se seleccionaron 27 artículos publicados entre 2010-2021, 5 guías clínicas y 3 libros. Los fármacos antihipertensivos con mayor reporte en la literatura fueron: los inhibidores de la enzima convertidora de angiotensina, los bloqueadores de canales de calcio y los diuréticos, siendo estos los que presentan una mayor cantidad de reacciones adversas en la cavidad oral reportadas. La xerostomía, las alteraciones del gusto y la hiperplasia gingival son las manifestaciones orales más frecuentes relacionadas con el uso de los antihipertensivos en esta revisión.

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“…The incidence of periodontitis in China as a whole is about 70%-85%. Periodontitis is a disease that endangers human oral health, and there is also a certain degree of correlation with the occurrence of hypertension [3]. Related studies have shown that periodontitis is related to the prevalence of hypertension, and the occurrence of hypertension will also cause the risk and severity of periodontitis.…”

Section: Introductionmentioning

confidence: 99%

Computed Tomography Three-Dimensional Reconstruction Algorithm in the Diagnosis of Periodontitis and Its Correlation with Hypertension

Pan

Liu

et al. 2022

Computational and Mathematical Methods in Medicine

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This study was aimed at exploring the value of iterative reconstruction (IR) algorithm to treat the periodontitis using computed tomography (CT) image and analyze the relationship between periodontitis and hypertension. 95 patients with periodontitis were selected, including 43 patients with periodontitis, 41 patients with advanced periodontitis and hypertension, and 11 patients with periodontitis and nonhigh blood pressure (NBP). The IR algorithm was introduced to the CT image scanning of them to reduce the noise. In addition, the CT value was statistically analyzed. High-sensitivity C-reactive protein (hs-CRP) and interleukin 6 (IL-6) were dramatically increased compared with periodontitis patients with NBP and with hypertension ( P < 0.05 ). After the IR algorithm of the image, the quality, information, and mean square error (MSE) of the image were all effectively improved. Image with a 50% dose showed the lowest noise, but the reconstruction algorithm improved the low-contrast resolution. Moderate and severe periodontitis was independently related to hypertension. Inflammatory cytokines were independently related to hypertension of periodontitis patients ( P < 0.05 ). Therefore, it was concluded that the IR algorithm could effectively improve the spatial resolution of the CT image when it was adopted to treat periodontitis and showed a high accuracy rate; the incidence of hypertension in patients with periodontitis was relatively high, and it had a certain relationship with periodontitis; and inflammatory cytokines were related to periodontitis and hypertension of patients.

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Allele-specific polymerase chain reaction for the detection of single nucleotide polymorphism in amlodipine-induced gingival enlargement

Abstract: Drug-induced complications can potentially be a result of genetic factors, in combination with various local and systemic factors. Identifying genetic polymorphisms early might help predict adverse reactions and determine prognosis.

Cited by 6 publications

References 10 publications

Identification and Cloning of a CC-NBS-NBS-LRR Gene as a Candidate of Pm40 by Integrated Analysis of Both the Available Transcriptional Data and Published Linkage Mapping

Identification and Cloning of a CC-NBS-NBS-LRR Gene as a Candidate of Pm40 by Integrated Analysis of Both the Available Transcriptional Data and Published Linkage Mapping

Medicación antihipertensiva y sus reacciones adversas en la cavidad oral. Una revisión integrativa

Computed Tomography Three-Dimensional Reconstruction Algorithm in the Diagnosis of Periodontitis and Its Correlation with Hypertension

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