Allelic and haplotype diversity of HLA‐A, HLA‐B and HLA‐DRB1 gene at high resolution in the Nanning Han population

Abstract

The distribution of human leucocyte antigen (HLA) allele and haplotype varied among different ethnic populations. In this study, we investigated the allele and haplotype frequencies of HLA-A, HLA-B and HLA-DRB1 loci in the Nanning Han population who live in Guangxi province of China. We identified 26 HLA-A, 56 HLA-B and 31 HLA-DRB1 alleles in 562 Nanning individuals of Han ethnic group by sequence-based typing method. Of these, the three most common alleles in HLA-A, HLA-B and HLA-DRB1 loci, respectively, were A*11:01 (32.12%), A*02:07 (12.54%), A*24:02 (12.01%); B*46:01 (14.41%), B*15:02 (13.61%), B*40:01 (11.48%); DRB1*15:01 (14.15%), DRB1*16:02 (11.57%) and DRB1*12:02 (10.14%). With the exception of HLA-DRB1, the p values of the HLA-A and HLA-B loci showed that the HLA allelic distribution in this population was in accordance with Hardy-Weinberg expectation (p > 0.05). A total of 173 HLA~A-B~DRB1 haplotype with a frequency of >0.1% were presented and the three most common haplotype were HLA-A*33:03~B*58:01~DRB1*03:01 (6.12%), HLA-A*11:01~B*15:02~DRB1*12:02 (3.39%) and HLA-A*11:01~B*15:02~DRB1*15:01 (3.22%). The phylogenetic tree and the principal component analysis suggested that Nanning Han population had a relative close genetic relationship with Chinese Zhuang population and a relative distant genetic relationship with Northern Han Chinese. The information will be useful for anthropological studies, for HLA matching in transplantation and disease association studies in the Chinese population.

The distributions of HLA‐A, HLA‐B, HLA‐C, HLA‐DRB1 and HLA‐DQB1 allele and haplotype at high‐resolution level in Zhejiang Han population of China

“…The frequencies of HLA‐A, HLA‐B, HLA‐C, HLA‐DRB1, and HLA‐DQB1 alleles were various among different populations in the previous reports (Buhler et al, ; Halagan et al, ; Kwok et al, ; Li et al, ; Loubser, Paximadis, & Tiemessen, ; Park et al, ; Pei et al, ; Schmidt et al, ; Wang et al, ; Yang, Chen, Shyr, & Lin, ). According to the previous studies, the distributions of HLA alleles were also difference between the Chinese southern Han population and northern Han population (Deng et al, ; Qin Qin et al, ; Shao et al, ).…”

“…Up to now, there were many reports available on the HLA‐A, HLA‐B and HLA‐DRB1 distribution in the Chinese populations (He et al, ; Li et al, ; Pei, Huang, Li, Chen, & Wu, ; Shao et al, ; Wang et al, ), but data on AFs and HFs of HLA‐C and HLA‐DQB1 are limited in Zhejiang Han population. In this study, we firstly reported the distribution of AFs and HFs of HLA‐A, HLA‐B, HLA‐C, HLA‐DRB1, and HLA‐DQB1 loci in Zhejiang Han population at a high‐resolution level.…”

Association of end‐stage renal disease with HLA phenotypes and panel reactive antibodies in patients awaiting renal transplantation in Hunan Province

“… 3 Because of the nonrandom expression patterns and linkage imbalance of HLA alleles, many studies have determined that the HLA phenotype of any individual has a certain correlation with that person's habitat region, race, disease occurrence, and PRA generation. 4 , 5 , 6 It has also been shown that the long‐term survival rate of a kidney graft increases if the HLA types match to a higher degree. 7 …”