ALLELIC COMPLEMENTATION AT THE <i>ad5/7</i> LOCUS IN YEAST

Dorfman, Ben-Zion

doi:10.1093/genetics/50.6.1231

Cited by 24 publications

(2 citation statements)

References 16 publications

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“…The three biosynthetic functions found in the gene in Drosophila, reside in two separate genes in yeast: ADE8 encodes GARTase and ADE5,7 encodes a bifunctional GARSase/AIRSase polypeptide (HENIKOFF 1986). In ADE5,7, GARSase-mutants form a simple complementation group, whereas AJRSase-mutants show a complex circular complementation pattern (COSTELLO and BEVAN 1964;DORFMAN 1964), which could represent the behavior of interacting subunits in a homodimeric protein (CRICK and ORGEL 1964). Given that the Drosophila analog contains duplicate and D. Nash AIRSase sequences, it will be interesting, ultimately, to discover whether mutants in the Drosophila AIRSase sequence yield a complex complementation pattern.…”

Section: Discussionmentioning

confidence: 99%

Genetic analysis of the adenosine3 (Gart) region of the second chromosome of Drosophila melanogaster.

Tiong

Nash

1990

Genetics

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The Gart gene of Drosophila melanogaster is known, from molecular biological evidence, to encode a polypeptide that serves three enzymatic functions in purine biosynthesis. It is located in polytene chromosome region 27D. One mutation in the gene (ade3(1)) has been described previously. We report here forty new ethyl methanesulfonate-induced mutations selected aga!nst a synthetic deficiency of the region from 27C2-9 to ++28B3-4. The mutations were characterized cytogenetically and by complementation analysis. The analysis apparently identifies 12 simple complementation groups. In addition, two segments of the chromosome exhibit complex complementation behavior. The first, the 28A region, gave three recessive lethals and also contains three known visible mutants, spade (spd), Sternopleural (Sp) and wingless (wg); a complex pattern of genetic interaction in the region incorporates both the new and the previously known mutants. The second region is at 27D, where seven extreme semilethal mutations give a complex complementation pattern that also incorporates ade3(1). Since ade3(1) is defective in one of the enzymatic functions encoded in the Gart gene, we assume the other seven also affect the gene. The complexity of the complementation pattern presumably reflects the functional complexity of the gene product. The phenotypic effects of the mutants at 27D are very similar to those described for ade2 mutations, which also interrupt purine biosynthesis.

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Section: Discussionmentioning

confidence: 99%

Genetic analysis of the adenosine3 (Gart) region of the second chromosome of Drosophila melanogaster.

Tiong

Nash

1990

Genetics

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“…Numerical values on a scale from 0 (noncomplementing at any time) to 99 (prolific growth in 24 hr) were arbitrarily assigned to every possible experimental record, with proportionality intended in terms of time and amount of growth. The averages of the pairwise replicates, from two to as many as eight tests of some allelic pairs, were computed, and those of greater value than the number assigned to weak growth by the seventh day were interpreted as complementing (2). Complementation of the regulatory function by color of diploids made homozygous for adel (R) was scored on FTLHA after I day on a six point scale from 0 (white) to 5 (dark red), by visual reference to a specific group of colonies chosen to serve as standards.…”

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confidence: 99%

Allelic Variability in Comparative Complementation Confirming that the adel2 -Specified Protein of Yeast is Bifunctional

Dorfman

1971

J Bacteriol

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Mutants at the adel2 locus of Saccharomyces cerevisiae are deficient in adenylosuccinate synthetase and are also responsible for constitutive purine biosynthetic activity. Ninety-six alleles were tested for restoration of each of these functions by allelic complementation and the results from the two types of test of every allele pair in the two matrices were compared with each other. Either the reactions were consistent, both complementing or noncomplementing, or inconsistent, with either enzymatic or regulatory function restored. The frequency and distribution of inconsistent behavior accords with the expectation that the adel2 locus specifies a bifunctional protein serving independent roles in enzymatic activity and in regulation of pathway activity. In a parallel experiment and analysis the addition of 1.0 m KCl increases the overall frequency of positive complementation, but skews the distribution of inconsistency sharply toward restoration of the regulatory function.

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Genetic analysis in the methylotrophic yeast Hansenula polymorpha

Gleeson

Sudbery

1988

Yeast

116

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Techniques are described for the induction, isolation, and characterization of mutants of Hansenula polymorpha. In addition, techniques for controlled passage through the life cycle and genetic analyses, including complementation, tetrad and random spore analysis, have been developed and used to assign mutants to 62 complementation groups. We report that organism conforms to the expected genetics of a homothallic yeast and displays a Mendelian segregation of genes through meiosis. Preliminary mapping data are presented indicating linkage of three genes on a single linkage fragment. Enymatic analysis of methanol‐non‐utilizing mutants identified one class which is totally deficient in the key assimilatroy enzyme, dihydroxyacetone synthase.

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ALLELIC COMPLEMENTATION AT THE ad5/7 LOCUS IN YEAST

Cited by 24 publications

References 16 publications

Genetic analysis of the adenosine3 (Gart) region of the second chromosome of Drosophila melanogaster.

Genetic analysis of the adenosine3 (Gart) region of the second chromosome of Drosophila melanogaster.

Allelic Variability in Comparative Complementation Confirming that the adel2 -Specified Protein of Yeast is Bifunctional

Genetic analysis in the methylotrophic yeast Hansenula polymorpha

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