Allelic Diversity of Acetyl Coenzyme A Carboxylase accD/bccp Genes Implicated in Nuclear-Cytoplasmic Conflict in the Wild and Domesticated Pea (Pisum sp.)

Abstract

Reproductive isolation is an important component of species differentiation. The plastid accD gene coding for the acetyl-CoA carboxylase subunit and the nuclear bccp gene coding for the biotin carboxyl carrier protein were identified as candidate genes governing nuclear-cytoplasmic incompatibility in peas. We examined the allelic diversity in a set of 195 geographically diverse samples of both cultivated (Pisum sativum, P. abyssinicum) and wild (P. fulvum and P. elatius) peas. Based on deduced protein sequences, we identified 34 accD and 31 bccp alleles that are partially geographically and genetically structured. The accD is highly variable due to insertions of tandem repeats. P. fulvum and P. abyssinicum have unique alleles and combinations of both genes. On the other hand, partial overlap was observed between P. sativum and P. elatius. Mapping of protein sequence polymorphisms to 3D structures revealed that most of the repeat and indel polymorphisms map to sequence regions that could not be modeled, consistent with this part of the protein being less constrained by requirements for precise folding than the enzymatically active domains. The results of this study are important not only from an evolutionary point of view but are also relevant for pea breeding when using more distant wild relatives.

Potential and limits of exploitation of crop wild relatives for pea, lentil, and chickpea improvement

“…The primary gene pool for domesticated pea (Harlan & de Wet, 1971) consists of the P. sativum / elatius complex (Smýkal et al, 2017; Trněný et al, 2018), although because of the existent nuclear–cytoplasmic conflict (Bogdanova, Galieva, & Kosterin, 2009; Nováková et al, 2019), there are some barriers to gene flow. A secondary gene pool (crosses with less success and lower fertility) extends to the other species in the genus, P. fulvum and P. abyssinicum .…”

Validation of a Triplex Quantitative Polymerase Chain Reaction Assay for Detection and Quantification of Traditional Protein Sources, Pisum sativum L. and Glycine max (L.) Merr., in Protein Powder Mixtures

“…from P. sativum L. but had a 0.76% divergence in between Pisum abyssinicum A. Braun and P. sativum L. Interestingly, accD emerged as one of the few regions with sufficient variability to discriminate among closely related species. Previous work has documented high variability due to insertions of tandem repeats and has linked the region to nuclear-cytoplasmic conflict in the wild and domesticated pea ( Nováková et al, 2019 ). Additionally, fragmentation and deletion of this region has been documented in several plant plastid genomes, with accD being completely deleted in the maize and wheat plastomes, and partially in rice ( Maier et al, 1995 ; Harris et al, 2013 ).…”

Genetic and Molecular Genetic Basis of Nuclear-Plastid Incompatibilities

“…truncatula., harbors the AccA locus coding for alpha subunit of carboxyltransferase of acetyl-CoA carboxylase [91]. Allelic polymorphism in the candidate genes was extensively studied [92].…”