Allelic prevalence of intron 3 insertion/deletion genetic polymorphism of DNA double-strand break repair gene XRCC4 in four healthy Iranian populations

Environ Health Prev Med

2016

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Section: Methodsmentioning

confidence: 99%

Influence of A-21T and C-262T genetic polymorphisms at the promoter region of the catalase (CAT) on gene expression

Environ Health Prev Med

2016

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“…A detailed description of these subjects has been reported in our previous report [ 27 ]. Considering that Iranian population is a heterogeneous population [ 28 – 30 ], we selected the participants from Persian (Caucasians) Muslims living in Shiraz (Fars province, south-west Iran). Informed consent was obtained from each volunteer before the study.…”

Section: Methodsmentioning

confidence: 99%

Influence of a 50bp Ins/Del polymorphism at promoter of the superoxide dismutase-1 on gene expression and risk of heroin dependency

Environ Health Prev Med

2017

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ObjectiveSuperoxide dismutase-1 (SOD1, OMIM: 147450) is one of the major antioxidant enzymes, which plays a vital role in clearance of reactive oxygen species. A genetic polymorphism of 50 bp insertion/deletion (Ins/Del) in the promoter region of the SOD1 was reported. The aims of the present study are to evaluate the influence of this polymorphism on the SOD1 mRNA levels in human peripheral blood cells and its association with risk of heroin dependency.MethodsThe present study consisted of 47 healthy students of Shiraz University (south-west Iran) for investigating the association between the Ins/Del polymorphism on expression level of SOD1, also a total of 442 heroin dependent and 799 healthy controls were included in a case-control study investigating the association between the study polymorphism and risk of dependency to heroin. The quantitative SOD1 mRNA expression levels were investigated using quantitative real-time PCR.ResultsStatistical analysis revealed a significant difference between the study genotypes (t = 5.17; df = 45; P < 0.001). The Del allele of the study polymorphism decreased approximately 33% of the SOD1 mRNA levels of the gene in the heterozygote individuals. Statistical analysis indicating that in both genders, neither the Ins/Del nor the Del/Del genotypes was associated with the risk of heroin addiction.ConclusionsThe present study indicating that although the Ins/Del polymorphism of SOD1 is associated with the SOD1 expression levels, this polymorphism is not associated with the risk of dependency to heroin.

“…Control individuals were blood donors, who declared that they did not suffer substance abuse. Considering the high heterogeneity of the Iranian population [ 16 - 18 ], the participants were selected from Persian Muslims (Caucasians) living in Shiraz (Fars Province, southern Iran). Informed consent was obtained from each subject before the study, which was approved by the institutional review board of our university.…”

Section: Methodsmentioning

confidence: 99%

Association between VNTR Polymorphism in Promoter Region of Prodynorphin (PDYN) Gene and Methamphetamine Dependence

Open Access Maced J Med Sci

2015

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AIM:Prodynorphin (PDYN; OMIM: 131340) is the precursor of the dynorphin related peptides which plays an important role in drug abuse. Previous studies have been shown that the expression of PDYN is regulated by a genetic polymorphism of VNTR in the promoter region of the gene.MATERIALS AND METHODS:The present case-control study was performed on 52 (41 males, 11 females) methamphetamine dependence patients and 635 (525 males, 110 females) healthy blood donors frequency matched with the patients according to age and gender, as a control group was participated in the study.RESULTS:The genotypes of VNTR PDYN polymorphism were determined using PCR method. The HL (OR = 1.22, 95%CI: 0.67-2.20, P = 0.500) and LL (OR = 0.86, 95%CI: 0.28-2.57, P = 0.792) genotypes does not alter the risk of methamphetamine dependence, in comparison with the HH genotypes.CONCLUSION:The present study revealed no association between the VNTR polymorphism in the promoter region of the PDYN gene and methamphetamine dependence risk.