Allelic variants of cytochrome P450 monooxygenases: Constitutive and insecticide-mediated expression in a Malaysian strain of the dengue vector, Aedes aegypti (Diptera: Culicidae)

El-garj, Fatma M. A.; Wajidi, Mustafa Fadzil Farid; Avicor, Silas Wintuma

doi:10.14411/eje.2016.067

Cited by 3 publications

(4 citation statements)

References 33 publications

(36 reference statements)

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“…AAEL002055, a neuroendocrine protein, was previously identified as carrying a single nucleotide showing significant spatial structure within a Brazilian population (Rašić et al 2015); CYP4H31 /AAEL002085 is a cytochrome P450 identified as overtranscribed in larvae selected to be resistant to the neonicotinoid insecticide imidacloprid (Riaz et al 2013). Its expression levels are altered in larvae exposed to DDT, malathion and temephos (El-Garj et al 2016), and it has been implicated in deltamethrin resistance in Culex (Li et al 2021).…”

Section: Resultsmentioning

confidence: 99%

“…Its expression levels are altered in larvae exposed to DDT, malathion and temephos (El-Garj et al 2016), and it has been implicated in deltamethrin resistance in Culex (Li et al 2021). Also on chromosome 2, a candidate sweep found only in the USA cohort extends from approximately 446 to 446.5 Mb, ("*c", Figure 2, Figure S6c).…”

Section: Strong Signatures Of Selective Sweeps In Insecticide Resista...mentioning

confidence: 99%

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Strong positive selection in Aedes aegypti and the rapid evolution of insecticide resistance

Love

Sikder

Vivero

et al. 2022

Preprint

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Aedes aegypti vectors the pathogens that cause dengue, yellow fever, Zika virus, and chikungunya, and is a serious threat to public health in tropical regions. Decades of work has illuminated many aspects of Ae. aegypti's biology and global population structure, and has identified insecticide resistance genes; however, the size and repetitive nature of the Ae. aegypti genome have limited our ability to detect positive selection in this mosquito. Combining new whole genome sequences from Colombia with publicly available data from Africa and the Americas, we identify multiple strong candidate selective sweeps in Ae. aegypti, many of which overlap genes linked to or implicated in insecticide resistance. We examine the voltage-gated sodium channel gene in three American cohorts, and find evidence for successive selective sweeps in Colombia. The most recent sweep encompasses an intermediate-frequency haplotype containing four candidate insecticide resistance mutations that are in near-perfect linkage disequilibrium with one another in the Colombian sample. We hypothesize that this haplotype may continue to rapidly increase in frequency and perhaps spread geographically in the coming years. These results extend our knowledge of how insecticide resistance has evolved in this species, and add to a growing body of evidence suggesting Ae. aegypti has an extensive genomic capacity to rapidly adapt to insecticide-based vector control.

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Section: Resultsmentioning

confidence: 99%

Section: Strong Signatures Of Selective Sweeps In Insecticide Resista...mentioning

confidence: 99%

Strong positive selection in Aedes aegypti and the rapid evolution of insecticide resistance

Love

Sikder

Vivero

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…One, CYP4H31 /AAEL002085, is a cytochrome P450 identified as upregulated in larvae selected to be resistant to the neonicotinoid insecticide imidacloprid ( Riaz et al 2013 ). Its expression levels are altered in larvae exposed to DDT, malathion, and temephos ( El-Garj et al 2016 ), and it has been implicated in deltamethrin resistance in Culex ( Li et al 2021 ).…”

Section: Resultsmentioning

confidence: 99%

Strong Positive Selection inAedes aegyptiand the Rapid Evolution of Insecticide Resistance

Love

Sikder

Vivero

et al. 2023

Molecular Biology and Evolution

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Aedes aegypti vectors the pathogens that cause dengue, yellow fever, Zika virus, and chikungunya, and is a serious threat to public health in tropical regions. Decades of work has illuminated many aspects of Ae. aegypti’s biology and global population structure, and has identified insecticide resistance genes; however, the size and repetitive nature of the Ae. aegypti genome have limited our ability to detect positive selection in this mosquito. Combining new whole genome sequences from Colombia with publicly available data from Africa and the Americas, we identify multiple strong candidate selective sweeps in Ae. aegypti, many of which overlap genes linked to or implicated in insecticide resistance. We examine the voltage-gated sodium channel gene in three American cohorts, and find evidence for successive selective sweeps in Colombia. The most recent sweep encompasses an intermediate-frequency haplotype containing four candidate insecticide resistance mutations that are in near-perfect linkage disequilibrium with one another in the Colombian sample. We hypothesize that this haplotype may continue to rapidly increase in frequency and perhaps spread geographically in the coming years. These results extend our knowledge of how insecticide resistance has evolved in this species, and add to a growing body of evidence suggesting Ae. aegypti has an extensive genomic capacity to rapidly adapt to insecticide-based vector control.

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“…Pyrethroid resistance is a major concern in Malaysia and two mechanisms of resistance (target-site resistance (also known as knockdown resistance, kdr ) and metabolic resistance) have been identified in Ae . aegypti [ 4 , 10 , 11 ], while metabolic detoxification and possibly reduced cuticular penetration have been implicated in resistance in Ae . albopictus [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Sodium Channel Mutations in the Dengue Vector Mosquitoes Aedes aegypti and Aedes albopictus within the Context of Ongoing Wolbachia Releases in Kuala Lumpur, Malaysia

Ahmad

Endersby‐Harshman

Mazni

et al. 2020

Insects

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Specific sodium channel gene mutations confer target site resistance to pyrethroid insecticides in mosquitoes and other insects. In Aedes mosquito species, multiple mutations that contribute to resistance vary in their importance around the world. Here, we characterize voltage sensitive sodium channel (Vssc) mutations in populations of Aedesaegypti from Kuala Lumpur, Malaysia, and look at their persistence in populations affected by ongoing Wolbachia releases (a dengue control measure). We also describe a Vssc mutation in Aedesalbopictus (F1534L) found for the first time in Malaysia. We show that there are three predominant Vssc haplotypes in Aedesaegypti in this region, which all persist with regular backcrossing, thereby maintaining the original genetic composition of the populations. We identify changes in genotype frequency in closed populations of Ae. aegypti maintained for multiple generations in laboratory culture, suggesting different fitness costs associated with the genotypes, some of which may be associated with the sex of the mosquito. Following population replacement of Ae. aegypti by Wolbachia in the target area, however, we find that the Vssc mutations have persisted at pre-release levels. Mosquitoes in two genotype classes demonstrate a type I pyrethroid resistance advantage over wildtype mosquitoes when exposed to 0.25% permethrin. This resistance advantage is even more pronounced with a type II pyrethroid, deltamethrin (0.03%). The results point to the importance of these mutations in pyrethroid resistance in mosquito populations and the need for regular backcrossing with male mosquitoes from the field to maintain similarity of genetic background and population integrity during Wolbachia releases.

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Allelic variants of cytochrome P450 monooxygenases: Constitutive and insecticide-mediated expression in a Malaysian strain of the dengue vector, Aedes aegypti (Diptera: Culicidae)

Cited by 3 publications

References 33 publications

Strong positive selection in Aedes aegypti and the rapid evolution of insecticide resistance

Strong positive selection in Aedes aegypti and the rapid evolution of insecticide resistance

Strong Positive Selection inAedes aegyptiand the Rapid Evolution of Insecticide Resistance

Characterization of Sodium Channel Mutations in the Dengue Vector Mosquitoes Aedes aegypti and Aedes albopictus within the Context of Ongoing Wolbachia Releases in Kuala Lumpur, Malaysia

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