2022
DOI: 10.1371/journal.pone.0276046
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Allelic variation of Escherichia coli outer membrane protein A: Impact on cell surface properties, stress tolerance and allele distribution

Abstract: Outer membrane protein A (OmpA) is one of the most abundant outer membrane proteins of Gram-negative bacteria and is known to have patterns of sequence variations at certain amino acids—allelic variation—in Escherichia coli. Here we subjected seven exemplar OmpA alleles expressed in a K-12 (MG1655) ΔompA background to further characterization. These alleles were observed to significantly impact cell surface charge (zeta potential), cell surface hydrophobicity, biofilm formation, sensitivity to killing by neutr… Show more

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Cited by 4 publications
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“…In addition, the periplasmic gap formed by disulfide linkages between OmpA’s β strands 4 and 5 in the N- and C-terminal fragments can increase the protein’s assembly efficiency in E. coli when the cysteine residues are aligned in the completely folded β-barrel [ 29 ]. The alleles of ompA were observed to have a considerable effect on cell surface hydrophobicity and charge in E. coli , which are important in stress response [ 30 ].…”
Section: Ompamentioning
confidence: 99%
“…In addition, the periplasmic gap formed by disulfide linkages between OmpA’s β strands 4 and 5 in the N- and C-terminal fragments can increase the protein’s assembly efficiency in E. coli when the cysteine residues are aligned in the completely folded β-barrel [ 29 ]. The alleles of ompA were observed to have a considerable effect on cell surface hydrophobicity and charge in E. coli , which are important in stress response [ 30 ].…”
Section: Ompamentioning
confidence: 99%