Allergen- and bacterial antigen-specific T-cell clones established from atopic donors show a different profile of cytokine production.

Parronchi, Paola; Macchia, Donatella; Piccinni, Marie‐Pierre; Biswas, Priscilla; Simonelli, Cecilia; Maggi, Enrico; Ricci, M; Ansari, Aftab A.; Romagnani, Sergio

doi:10.1073/pnas.88.10.4538

Cited by 571 publications

(284 citation statements)

References 27 publications

Supporting

Mentioning

270

Contrasting

Unclassified

Order By: Relevance

“…The same is true for T cell clones derived from lesional atopic dermatitis skin or from bronchial mucosa from atopic asthma patients [8][9][10][11]. From the peripheral blood of atopic patients, allergen-specific Th2 clones could also be generated [12][13][14]. Even when T cell clones were generated by polyclonal stimulation, or by antigens not related to allergy, the frequency of IL-4-producing clones was higher in atopic patients than in normal subjects [15.16].…”

Section: Introductionmentioning

confidence: 74%

IgE production in atopic patients is not related to IL-4 production

Kraan¹,

Aalberse²,

Aarden³

1994

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SUMMARYTo analy.se whether there is a general defect in T or B cell function in atopic individuals we have measured cytokine and IgE production by peripheral blood lymphocytes, isolated from 19 atopic donors (17 asthma/rhinitis and two dermatitis patients) in comparison with 19 non-atopic controls. After stimulation oflymphocytes with aiiti-CD2 and anti-CD28. we found no significant difference in IL-2, IL-4 and interferon-gamma (IEN--)) production. To examine the correlation between the production of IgE and IL-4, we stimulated lymphocytes with anti-CD2 and rIL-2. Under this condition both T eell iL-4 and B cell IgE production ean be measured. No significant diflerence was found for the amount of IgE and IL-4 produced between the two groups ( P > 005). The non-atopie donors showed a good correlation between IL-4 and IgE production (r = 0'70). Surprisingly, within the atopic group there was no correlation between IgE and IL-4 production at all (r = -0-04). The ratio of IgE to IL-4 was higher (although not signifieatitly) in the atopic group. Our data suggest that in atopic donors IgE production is less dependent on IL-4, and that other cytokines are involved.

show abstract

Section: Introductionmentioning

confidence: 74%

IgE production in atopic patients is not related to IL-4 production

Kraan¹,

Aalberse²,

Aarden³

1994

Clinical and Experimental Immunology

View full text Add to dashboard Cite

show abstract

“…Atopic disease is characterized by an individual predisposition to develop IgE mediated allergies against environmental allergens (Ishizaka and Ishizaka, 1971) and a Th2-polarized immunity (Del Prete et al, 1993). Atopic patients have an enhanced ability to produce IL-4, not only in response to allergens, but also to other antigens (Parronchi et al, 1992), and in line with this, atopic disease is associated with low IFN-γ secretion (Parronchi et al, 1991;Shimojo et al, 1996)). However, we have previously found an IL-4 response to birch only in birch sensitized patients but not in cat sensitized patients, and vice versa (Jenmalm et al, 2001).…”

Section: Introductionmentioning

confidence: 94%

Increased circulating paternal antigen-specific IFN-γ- and IL-4-secreting cells during pregnancy in allergic and non-allergic women

Persson

Ekerfelt

Ernerudh

et al. 2008

Journal of Reproductive Immunology

View full text Add to dashboard Cite

show abstract

“…Growing microcultures were then expanded at weekly intervals with fresh feeder cells and r IL-2. The specificity of TCC was assessed as previously described [12]. Briefly, 2 X lo4 T-cell blasts were incubated in triplicate cultures in 96 well plates in the presence of lo5 autologous PBMC, as antigen-presenting cells (APC), purified Bet v 1 and whole birch pollen extract.…”

Section: Allergen-specific Tccmentioning

confidence: 99%

“…polymerase chain reaction (PCR) as described [28-301. Analysis of TCR VP region was performed using the Peptides Epitope mapping of TCC was performed using dodecapeptides overlapping for two residues (neighbours share 10 amino acids) as described previously [12]. Peptides representing homologue isoepitopes on the four Cor a 1 isoallergens were synthesized (Cambridge Research Biochemicals Limited).…”

Section: Tcr Analysismentioning

confidence: 99%

See 1 more Smart Citation

Four Recombinant Isoforms of Cor a 1, the Major Allergen of Hazel Pollen, Show Different Reactivities with Allergen‐specific T‐lymphocyte Clones

Schenk

Hoffmann‐Sommergruber

Breiteneder

et al. 1994

European Journal of Biochemistry

View full text Add to dashboard Cite

Purified preparations of allergenic proteins from plants, and in particular from pollens, consist of multiple closely related isoforms. These isoforms are highly similar in their amino acid sequences, yet they display different properties with respect to antibody binding. In this study we report of differential potencies of cross-reacting tree pollen allergens and cloned isoforms of these allergens to activate allergen-specific T-lymphocyte clones (T-cell clones ; TCC). Six TCC with specifity for Bet v 1, a representative tree pollen major allergen, were established from peripheral blood of five birch-pollen-allergic donors. All TCC displayed the helper-cell phenotype. Five TCC reacted with distinct epitopes present on natural (n) and on recombinant (r) Bet v 1. One TCC could not be stimulated with r Bet v 1, in spite of strong reactivity with purified natural Bet v 1. The TCC were tested in proliferation assays using purified n Bet v 1, n Cor a 1 (the homologous major allergen of hazel pollen), r Bet v 1, four recombinant isoforms of Cor a 1 and peptides representing corresponding T-cell stimulating regions (isoepitopes) on these proteins. The clones showed different patterns of reactivity in response to stimulation with the five recombinant molecules and the corresponding peptides. Certain exchanges of amino acids within stimulating peptides correlated with a lack of proliferation of the TCC tested. These findings are important with respect to the use of broadly cross-reactive recombinant allergens or allergen-derived peptides for immunotherapy of type I allergy.Type I allergy to tree pollens is very common in the northern hemisphere. In this respect, pollens from trees of the order Fagales, in particular birch, hazel and alder, represent the most important sources of allergens [l, 21. The major allergens of these pollens (Bet v 1, Cor a 1 and A h g 1) show a high degree of similiarity at the nucleic acid 131 and at the amino acid levels [4], and cross-reactivity was described for IgE antibodies [5] and for T-cell clones (TCC) [6]. This fits well with the observation that tree pollen-allergic patients develop symptoms when exposed to pollens from all these tree species.Natural allergens consist of multiple isoallergens [7 -91. Recently, four isoforms of Cor a 1, the major allergen of hazel pollen, were cloned, sequenced and expressed as recombinant non-fusion proteins [8]. Comparison of the amino acid sequences of these Cor a 1 isoforms revealed identities Correspondence to

show abstract

Allergen- and bacterial antigen-specific T-cell clones established from atopic donors show a different profile of cytokine production.

Cited by 571 publications

References 27 publications

IgE production in atopic patients is not related to IL-4 production

IgE production in atopic patients is not related to IL-4 production

Increased circulating paternal antigen-specific IFN-γ- and IL-4-secreting cells during pregnancy in allergic and non-allergic women

Four Recombinant Isoforms of Cor a 1, the Major Allergen of Hazel Pollen, Show Different Reactivities with Allergen‐specific T‐lymphocyte Clones

Contact Info

Product

Resources

About