Allergen Microarray Indicates Pooideae Sensitization in Brazilian Grass Pollen Allergic Patients

Moreira, Priscila Ferreira de Sousa; Gangl, Katharina; Vieira, Francisco de Assis Machado; Ynoue, Leandro Hideki; Linhart, Birgit; Flicker, Sabine; Fiebig, Helmut; Swoboda, Ines; Focke‐Tejkl, Margarete; Taketomi, Ernesto Akio; Valenta, Rudolf; Niederberger, Verena

doi:10.1371/journal.pone.0128402

Cited by 7 publications

(15 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several scientists have investigated the importance of grass pollen as outdoor aeroallergen, focusing on the mechanisms of patient sensitization and therapies (Durham et al, 1999;Moreira et al, 2015;Tripodi et al, 2012), relationship between grains/allergens concentration and symptoms (Annesi-Maesano et al, 2012;Erbas et al, 2012;Feo Brito et al, 2010), patterns of emission (Emberlin et al, 2000;Fernández Rodríguez et al, 2014;Galán, Emberlin, Domínguez, Bryant, & Villamandos, 1995;Ghitarrini, Galán, Frenguelli, & Tedeschini, 2017;Sánchez-Mesa et al, 2003), and influence of meteorological factors on the pollen season timing and intensity (García-Mozo, Mestre, & Galán, 2010;Ghitarrini, Tedeschini, Timorato, & Frenguelli, 2017;Smith et al, 2009;Zhang et al, 2015). The grasses that mostly contribute to the airborne pollen load vary spatially, but those responsible for grass pollinosis are essentially included into a group of about 20 species, principally belonging to the subfamily Pooideae.…”

Section: Introductionmentioning

confidence: 99%

New biomolecular tools for aerobiological monitoring: Identification of major allergenic Poaceae species through fast real‐time PCR

Ghitarrini

Pierboni

Rondini

et al. 2018

Ecology and Evolution

View full text Add to dashboard Cite

Grasses (Poaceae) are very common plants, which are widespread in all environments and urban areas. Despite their economical importance, they can represent a problem to humans due to their abundant production of allergenic pollen. Detailed information about the pollen season for these species is needed in order to plan adequate therapies and to warn allergic people about the risks they take in certain areas at certain moments. Moreover, precise identification of the causative species and their allergens is necessary when the patient is treated with allergen‐specific immunotherapy. The intrafamily morphological similarity of grass pollen grains makes it impossible to distinguish which particular species is present in the atmosphere at a given moment. This study aimed at developing new biomolecular tools to analyze aerobiological samples and identifying major allergenic Poaceae taxa at subfamily or species level, exploiting fast real‐time PCR. Protocols were tested for DNA extraction from pollen sampled with volumetric and gravimetric methods. A fragment of the matK plastidial gene was amplified and sequenced in Poaceae species known to have high allergological impact. Species‐ and subfamily‐specific primer–probe systems were designed and tested in fast real‐time PCRs to evaluate the presence of these taxa in aerobiological pollen samples. Species‐specific systems were obtained for four of five studied species. A primer–probe set was also proposed for the detection of Pooideae (a grass subfamily that includes also major cereal grains) in aerobiological samples, as this subfamily includes species carrying both grass allergens from groups 1 and 5. These, among the 11 groups in which grass pollen allergens are classified, are considered responsible for the most frequent and severe symptoms.

show abstract

Section: Introductionmentioning

confidence: 99%

New biomolecular tools for aerobiological monitoring: Identification of major allergenic Poaceae species through fast real‐time PCR

Ghitarrini

Pierboni

Rondini

et al. 2018

Ecology and Evolution

View full text Add to dashboard Cite

show abstract

“…A fim de avaliar o perfil de bandas do extrato proteico de L. multiflorum (S1), após a extração alergênica, foi realizada a eletroforese em gel de poliacrilamida (SDS-PAGE). O perfil eletroforético foi semelhante ao encontrado em estudos prévios do nosso grupo (BERNARDES et al, 2010;MOREIRA et al, 2015). De todas as bandas detectadas, as bandas de 12, 32 e 58 kDa, foram as bandas com maior intensidade, com maior destaque para a banda de 32 kDa.…”

Section: Discussionunclassified

“…Dentre essas proteínas, os alérgenos do grupo 1, que estão presentes em todas as subfamílias de Poaceae, e são denominados -expansinas podem constituir até 10% de todo grão de pólen. Como representantes dessas proteínas se destacam, (BERNARDES et al, 2010;MOREIRA et al, 2015;SOPELETE et al, 2006). Um recente estudo, mostrou que L.…”

Section: Polensunclassified

“…Phleum pratense, apresentando reatividade cruzada de anticorpos IgE de até 95% com os antígenos dessa espécie (BERNARDES et al, 2010;MOREIRA et al, 2015;SOPELETE et al, 2006;FERREIRA;MATTER, 2005 (GEILFUS et al, 2015;LI et al, 2003;ROSMILAH et al, 2013;WANG et al, 2016…”

Section: Polensunclassified

“…positivos para S1, tenham sido negativos para S2. Por isso, interpretamos que a cromatografia de troca iônica, tenha isolado os alérgenos principais de L. multiflorum, mas se na fração S2 continua ou não existindo componentes responsáveis por reatividades cruzadas ainda necessitam de futuras elucidações, uma vez que as nossas observações anteriores têm demonstrado que L. multiflorum apresenta alta reatividade cruzada com diversas outras gramíneas, tais como Phleum pratense e Cynodon dactylon(MOREIRA et al, 2015).Para estabelecer o perfil de reatividade do extrato S1 e da fração S2 foi realizado a técnica de Immunoblotting. As bandas de peso molecular relativode 12, 26, 29, 32, 39, 58 e 69 kDa foram as mais reativas nos pools de soros de indivíduos atópicos frente a S1, todas com mais de 50% de reatividade a anticorpos da classe IgE, com maior destaque para a banda de 32 kDa, que se ligou a 100% dos soros dos pacientes alérgicos, sugerindo a sua grande importância.Da mesma forma, a banda de 32 KDa, na fração S2, obteve a mesma impressionante reatividade a anticorpos da classe IgE em soros dos indivíduos alérgicos.…”

unclassified

See 2 more Smart Citations

Perfil de proteínas antigênicas derivadas do extrato total de polens de lolium multiflorum e de sua fração reconhecidas por anticorpos das classes ige e igg4 de pacientes atópicos com potencial diagnóstico e terapêutico

Correa¹

View full text Add to dashboard Cite

Recombinant allergen and peptide-based approaches for allergy prevention by oral tolerance

Campana

Huang

Freidl

et al. 2017

Seminars in Immunology

View full text Add to dashboard Cite

Several studies conducted in animal models for immunologically-mediated hypersensitivity diseases have shown that oral administration of antigens early in life can prevent the development of specific humoral and cellular immune responses and thus hypersensitivity reactions to the respective antigens. Such data were also obtained in models for Immunoglobulin E (IgE)-associated allergy, the most common hypersensitivity disease affecting more than 25% of the population. Based on data obtained in animal models for allergy several clinical intervention studies have been conducted in children to study if oral administration of materials containing allergens or allergen-derived peptides early in life can prevent the subsequent development of allergy. In this article we argue that oral tolerance induction could be a potent way to prevent allergy and may be even improved regarding efficacy provided that well-defined allergen molecules and/or allergen-derivatives were used in optimized dose regimens and periods of intervention. The knowledge regarding the molecular and immunological characteristics of allergens which has been achieved in the last decades is a prerequisite for such a treatment. In fact, defined recombinant allergens/allergen derivatives and allergen-derived synthetic peptides from the most common allergen sources are now available for targeted intervention. Moreover, molecular allergy diagnosis allows deciphering the disease-causing relevant allergens for different regions in the world allowing composing cocktails of tolerogens according to the needs of populations from different parts of the world. Furthermore, it is suggested to use defined allergen molecules and epitopes in the analysis of clinical tolerance studies. This will allow understanding if clinical unresponsiveness is due to true immunological tolerance or to other mechanisms such as induction of blocking antibodies or cellular immunomodulation. Using molecularly defined tolerogens it can now be explored if oral tolerance induction is a powerful strategy to prevent IgE-associated allergy.

show abstract

Allergen Microarray Indicates Pooideae Sensitization in Brazilian Grass Pollen Allergic Patients

Cited by 7 publications

References 27 publications

New biomolecular tools for aerobiological monitoring: Identification of major allergenic Poaceae species through fast real‐time PCR

New biomolecular tools for aerobiological monitoring: Identification of major allergenic Poaceae species through fast real‐time PCR

Perfil de proteínas antigênicas derivadas do extrato total de polens de lolium multiflorum e de sua fração reconhecidas por anticorpos das classes ige e igg4 de pacientes atópicos com potencial diagnóstico e terapêutico

Recombinant allergen and peptide-based approaches for allergy prevention by oral tolerance

Contact Info

Product

Resources

About