“…Using a panel of 18 Brazil nut-specific allergic subjects and a chimearic protein microarray format, we have identified the structural immunodominant region of Ber e 1. Other studies using hybrid proteins have been reported recently; 8,9 however, we show here that the combination of chimearic protein design in an eukaryotic system and microarray screening is a promising platform for generating large numbers of structural epitopes.…”