Allergic inflammation is exacerbated by allergen-induced type 2 innate lymphoid cells in a murine model of allergic rhinitis

Lin, Lan; Dai, Fei; Wei, Jinjin; Tang, Xinyue; Chen, Zhi Hao; Sun, Guangbin

doi:10.4193/rhin17.065

Cited by 13 publications

(14 citation statements)

References 23 publications

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“…We also found that the adoptive transfer of NALT-derived nuocytes into the murine AR model exacerbated the allergic inflammation, just like another study from us. 17 Numbers of sneezing, nasal rubbing and infiltrating eosinophils, and expressing levels of IL-5, IL-13, and IL-33 in the NLF were all enhanced significantly when compared to those of the AR group. However, the aforementioned parameters were all reduced after the adoptive transfer of nuocytes transfected by lenti-ORAI1.…”

Section: Discussionmentioning

confidence: 85%

“…27 A previous study from us demonstrated that allergic inflammation could be exacerbated by OVA-induced nuocytes in a murine model of AR. 17 However, some characteristics of these cells could not be further investigated in that research. In this present study, we aimed to determine Orai1 expression in nuocytes and evaluate the influence of intervention of Orai1 on the response of these cells from AR mice to IL-33.…”

Section: Discussionmentioning

confidence: 95%

“…12 Our previous studies showed that nuocytes could be induced by ovalbumin (OVA) in the nasal-associated lymphoid tissue (NALT) of the murine AR model and could produce IL-5 and IL-13 after the administration of recombinant (rm) IL-25. 17 However, it remains to be studied whether these cell types express Orai1, the key component of Ca 2+ release-activated Ca 2+ (CRAC) channel in the plasma membrane of various cells, and the function of this protein in nuocytes. CRAC channel is a typical store-operated Ca 2+ entry (SOCE) pathway that is activated by the depletion of Ca 2+ stores in endoplasmic reticulum (ER) and controls the cellular functions through the activation of Ca 2+ signaling pathway in immune system cells.…”

Section: Introductionmentioning

confidence: 99%

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Intervention of Orai1 Influences the Response of Nuocytes From Allergic Rhinitis Mice to IL-33

Lin

Wei

Chen

et al. 2019

Ann Otol Rhinol Laryngol

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Objective: Nuocytes are essential in innate type-2 immunity and contribute to the exacerbation of allergic rhinitis (AR). This study aimed to evaluate the intervention of Orai1 on the response of nuocytes from AR mice to interleukin (IL)-33. Methods: We established a murine model of AR. Nuocytes were obtained from the mouse nasal-associated lymphoid tissue. Then, we assessed expressions of Orai1, Ca2+ mean fluorescence intensity (MFI) in nuocytes, and their cellular response to mouse recombinant (rm) IL-33. After that, we administered rmlentivirus vectors (lenti) that encoded small hairpin RNA (shRNA) against ORAI1 (lenti-ORAI1) into nuocytes cultures and again evaluated Orai1 and Ca2+ MFI in nuocytes and their response to rmIL-33. Finally, we adoptively transferred nuocytes alone or nuocytes transfected by lenti or lenti-ORAI1 to AR models to investigate their roles during allergic inflammation. Results: We showed that Orai1 and Ca2+ MFI were upregulated in AR mice nuocytes. These cells were induced to produce more IL-5 and IL-13 by rmIL-33. However, the intervention of Orai1 by lenti-ORAI1 in nuocytes decreased Orai1 and Ca2+ MFI and reduced productions of aforementioned cytokines even after the administration of rmIL-33. Numbers of sneezing, nasal rubbing, and counts of eosinophils were all enhanced after the adoptive transfer of nuocytes. Concentrations of IL-5, IL-13, and IL-33 in the nasal lavage fluid (NLF) of allergic mice were also increased. However, the adoptive transfer of nuocytes transfected by lenti-ORAI1 decreased aforementioned parameters. Conclusion: These findings show that the intervention of Orai1 in nuocytes influences the response of nuocytes to rmIL-33.

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Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

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Intervention of Orai1 Influences the Response of Nuocytes From Allergic Rhinitis Mice to IL-33

Lin

Wei

Chen

et al. 2019

Ann Otol Rhinol Laryngol

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“…In addition, Eastman et al found that ILC2s were recruited to the nasal mucosa in patients with aspirin‐exacerbated respiratory disease. Using murine models, two recent researches confirmed the important role of ILC2s in exacerbating type 2 inflammation in AR …”

Section: Introductionmentioning

confidence: 89%

Novel innate and adaptive lymphocytes: The new players in the pathogenesis of inflammatory upper airway diseases

Liu

Yao

Wang

et al. 2018

Clin Experimental Allergy

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Host immunity (innate and adaptive immunity) plays essential roles in the pathogenesis of inflammatory upper airway diseases, including allergic rhinitis and chronic rhinosinusitis. Recently, the discovery of novel innate immune cells, particularly innate lymphoid cells, has renewed our view on the role of innate immunity in inflammatory upper airway diseases. Meanwhile, the identification of new subsets of T helper (Th) cells, including Th22, Th9 and follicular Th cells, and regulatory B cells in the adaptive immunity, has broadened our knowledge on the complex immune networks in inflammatory upper airway diseases. In this review, we focus on these newly identified innate and adaptive lymphocytes with their contributions to the immunological disturbance in allergic rhinitis and chronic rhinosinusitis. We further discuss the perspective for future research and potential clinical utility of regulating these novel lymphocytes for the treatment of allergic rhinitis and chronic rhinosinusitis.

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“…Apart from the EUFOREA report, we have a lot of very interesting papers on diverse subjects from allergic rhinitis (19,20) , via risk factors for epistaxis (21) , to surgical papers on inverted papilloma (22) and CSF leak repair (23) .…”

mentioning

confidence: 99%

The future in Rhinology: from local treatment, to monoclonals and influencing the microbiome

Fokkens

2017

Rhin

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Allergic inflammation is exacerbated by allergen-induced type 2 innate lymphoid cells in a murine model of allergic rhinitis

Abstract: These findings show that ILC2s play a proinflammatory role in the murine AR model, and also highlight ILC2s as a new target in the future AR therapy.

Cited by 13 publications

References 23 publications

Intervention of Orai1 Influences the Response of Nuocytes From Allergic Rhinitis Mice to IL-33

Intervention of Orai1 Influences the Response of Nuocytes From Allergic Rhinitis Mice to IL-33

Novel innate and adaptive lymphocytes: The new players in the pathogenesis of inflammatory upper airway diseases

The future in Rhinology: from local treatment, to monoclonals and influencing the microbiome

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