Allergy to plant‐derived fresh foods in a birch‐ and ragweed‐free area

Cuesta‐Herranz, Javier; Lázaro, Milagros; Figueredo, E; Igea, J; Umpiérrez, Ana; de-Las-Heras, Manuel

doi:10.1046/j.1365-2222.2000.00868.x

Cited by 73 publications

(60 citation statements)

References 27 publications

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“…Peach allergy mediated by Pru p 3 can be related with mild reactions restricted to the orapharyngeal cavity (oral allergy syndrome, OAS), frequently associated with pollinosis (Ortolani et al, 1988;Cuesta-Herranz et al, 2000;Zuidmeer and van Ree, 2007;Fernandez-Rivas et al, 2003). Besides, severe systemic symptoms are developed by many patients (around 30%) suffering this allergy, with or without associated pollinosis (Zuidmeer and van Ree, 2007;Fernandez-Rivas et al, 2003;FernandezRivas et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

“…Patients were selected at the Servicio de Alergia, Fundacion Jimenez Diaz (Madrid) based on a convincing clinical history of allergic reactions after peach ingestion and a positive skin prick test (SPT) (Sub-Committee on Skin Tests of the European Academy of Allergology and Clinical Immunology, 1989) to response to peach (ALK-Abello, Madrid, Spain). On a second step, peach allergy was confirmed in all selected patients by an open oral challenge test to peach following the method previously described (Cuesta-Herranz et al, 1998;Cuesta-Herranz et al, 2000). Finally, positive SPT response and specific IgE to Pru p 3 were also need to take part in the study.…”

Section: Patients and Seramentioning

confidence: 99%

“…Fruits, particularly peach, are the foods most frequently involved in allergic reactions in the adult population from the Mediterranean area (Ortolani et al, 1988;Kanny et al, 2001;Cuesta-Herranz et al, 1998;Cuesta-Herranz et al, 2000). The major peach allergen, named Pru p 3, belongs to the lipid transfer protein (LTP) panallergen family (Sanchez-Monge et al, 1999;Pastorello et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Mimotope mapping as a complementary strategy to define allergen IgE-epitopes: Peach Pru p 3 allergen as a model☆

Pacios¹,

Tordesillas²,

Cuesta‐Herranz

et al. 2008

Molecular Immunology

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Lipid transfer proteins (LTPs) are the major allergens of Rosaceae fruits in the Mediterranean area. Pru p 3, the LTP and major allergen of peach, is a suitable model for studying food allergy and amino acid sequences related with its IgE-binding capacity. In this work, we sought to map IgE mimotopes on the structure of Pru p 3, using the combination of a random peptide phage display library and a three-dimensional modelling approach. Pru p 3-specific IgE was purified from 2 different pools of sera from peach allergic patients grouped by symptoms (OAS-pool or SYS-pool), and used for screening of a random dodecapeptide phage display library. Positive clones were further confirmed by ELISA assays testing individual sera from each pool. Three-dimensional modelling allowed location of mimotopes based on analysis of electrostatic properties and solvent exposure of the Pru p 3 surface. Twenty-one phage clones were selected using Pru p 3-specific IgE, 9 of which were chosen using OAS-specific IgE while the other 12 were selected with systemic-specific IgE. Peptide alignments revealed consensus sequences for each pool: L37 R39 T40 P42 D43 R44 A46 P70 S76 P78 Y79 for OAS-IgE, and N35 N36 L37 R39 T40 D43 A46 S76177 P78 for systemic-IgE. These 2 consensus sequences were mapped on the same surface of Pru p 3, corresponding to the helix 2-loop-helix 3 region and part of the non-structured C-terminal coil. Thus, 2 relevant conformational IgE-binding regions of Pru p 3 were identified using a random peptide phage display library. Mimotopes can be used to study the interaction between allergens and IgE, and to accelerate the process to design new vaccines and new immunotherapy strategies.

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Section: Discussionmentioning

confidence: 99%

Section: Patients and Seramentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Mimotope mapping as a complementary strategy to define allergen IgE-epitopes: Peach Pru p 3 allergen as a model☆

Pacios¹,

Tordesillas²,

Cuesta‐Herranz

et al. 2008

Molecular Immunology

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“…Many reasonably standardized pollen extracts are commercially available [3]. Most allergen preparations derived from complex allergenic sources like fungi, moulds, yeasts, food allergens and some pollens are still not yet available as internationally recognized, standardized products [2, 3]. In contrast to most pollen and Hymenoptera venoms, these allergenic sources contain complex patterns of allergenic molecules ranging from 20 to over 80 different molecular structures [25, 26].…”

Section: Use Of Crude Allergens or Allergen Extracts For Skin Testingmentioning

confidence: 99%

“…A wide choice of skin test solutions is commercially available, including numerous inhalant, food and other allergens. Also crude material such as raw food (prick-to-prick technique) or samples of house dust or animal dander can be used as test solution [2]. Skin tests have also been shown to be generally safe, sensitive and reliable if standardized allergen extracts are used.…”

Section: Introductionmentioning

confidence: 99%

Recombinant Allergens for Skin Testing

Schmid‐Grendelmeier¹,

Crameri²

2001

Int Arch Allergy Immunol

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Skin testing is a basic diagnostic procedure widely used to explore immediate-type reactions to allergen preparations in vivo. Despite their reliability, if standardized extracts are used, skin tests suffer from limited reproducibility due to difficulties in preparing consistently standardized extracts from natural raw material. Starting from allergen-encoding cDNAs, large amounts of highly pure allergens with a high batch-to-batch consistency satisfying the quality requirements of medicinal products manufactured by recombinant DNA technology can be produced. These reagents are expected to be qualitatively superior to the commercially available allergen preparations used for the in vitro and in vivo diagnosis of allergic conditions. In this article the current literature available on skin testing with such recombinant allergens (rAllergens) is reviewed and critically analyzed. To date many different rAllergens of various pollens, moulds, mites, bee venom, latex and celery have been used in skin testing in more than 1,600 allergic and control individuals. Skin prick tests as well as intradermal skin tests with rAllergens prove to be highly specific and safe. The diagnostic sensitivity of single rAllergens is generally lower than those obtained with allergen extracts, but can be considerably increased by using rAllergen panels covering the most important allergenic structures present in a given complex allergenic extract. Moreover, quantitative skin testing with single rAllergens allows interesting insights into correlations between the in vivo and in vitro sensitization to a given allergen. In conclusion, skin testing with rAllergens offers a highly specific and safe additional diagnostic tool to elucidate patient- and disease-specific sensitization patterns which will be needed for the development of patient-tailored immunotherapeutic treatments.

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Oral Allergy Syndrome

Wang

2013

Food Allergy

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Allergy to plant‐derived fresh foods in a birch‐ and ragweed‐free area

Abstract: Peach was the most important allergy provoking fruit in a birch and ragweed free-area where apples were consumed at a rate of two times more than peaches and the patients allergic to pollen were principally sensitized to grass pollens.

Cited by 73 publications

References 27 publications

Mimotope mapping as a complementary strategy to define allergen IgE-epitopes: Peach Pru p 3 allergen as a model☆

Mimotope mapping as a complementary strategy to define allergen IgE-epitopes: Peach Pru p 3 allergen as a model☆

Recombinant Allergens for Skin Testing

Oral Allergy Syndrome

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