Alleviation of bone markers in rats induced nano-zinc oxide by qurecetin and α-lipolic acid

Abstract

The purpose of this study was to evaluate the potential protective effect of qurecetin (Qur) and α-lipolic acid (ALA) to modulate the perturbation of bone turnover which is induced by nano-zinc oxide (n-ZnO). Rats were fasted overnight and randomly divided into two groups: G1, normal healthy animals and the other rats were administered zinc oxide nanoparticles orally by guava in a dose of 600 mg/kg body weight/d for 5 sequential days in Wistar albino male rats. N-ZnO-exposed animals were randomly sub-divided into three groups: G2, n-ZnO-exposed animals; G3, n-ZnO-exposed animals co-treated with Qur (200 mg/kg daily); and G4, n-ZnO-exposed animals co-treated with ALA (200 mg/kg). Qur and ALA were administered orally by guava daily for three sequential weeks from the beginning of the experiment. The results revealed a significant reduction of nitiric oxide (NO) and serum level and comet assay in n-ZnO exposure rats after treatment of Qur and ALA. It was found the alteration of pro-inflammatory markers (tumor necrosis factor alpha; TNF-α, interleukin-6; IL-6 and C-reactive protein; CRP), bone alkaline phosphatase (B-ALP, bone formation marker), and C-terminal peptide type I collagen (CTx, bone resorption marker) levels compared with the normal group. Co-administration of Qur and ALA in n-ZnO-exposed rats significantly alleviated the mentioned alterations of biochemical parameters. These results suggest that Qur and ALA as antioxidant agents may be a candidate for preventive and treatment applications of impaired bone markers induced bone loss caused by nano-particles of metal oxide.

The effect of white kidney bean fertilized with nano-zinc on nutritional and biochemical aspects in rats

“…Moreover, Many studies have documented that high doses of ZnO-NPs can cause apoptosis in liver and produce oxidative stress [23] and also they can induce liver DNA damage [24]. Moreover, a high dose of ZnO-NPs can induce bone resorption in rats [25]. In addition, Han et al [26] proved that ZnO-NPs may have an adverse effect on the rat's hippocampus.…”

Quercetin as an Agent for Protecting the Bone: A Review of the Current Evidence

“…Quercetin reduced the levels of IL-1β, TNF-α and IL-6 while increasing those of IL-10 and Arg-1 in RAW264.7 cells exposed to M-CSF, RANKL or LPS [45,46,99]. In vivo, a reduction in TNF-α, IL-6 and CRP levels was observed following quercetin or quercetin-loaded phytosome nanoparticle interventions in ovariectomised [30] and zinc-oxide-nanoparticle-treated rats [44].…”

“…STZ-induced diabetic rats orally treated with quercetin for 8 weeks [39]. Along with the elevation of bone formation markers and reduction in bone resorption markers, Abdelkarem et al (2016) also found a decrease in NO• and DNA damage in rats exposed to zinc oxide nanoparticles and treated with quercetin [44]. Nonetheless, no effect was observed in oxidative-stress biomarkers and antioxidant activities when quercetin was orally supplemented to healthy rats [43].…”

“…Exposure to zinc oxide nanoparticles caused perturbation of bone turnover, reflected by alteration of bone formation and bone resorption markers. The bone ALP level was higher and serum CTX level was lower in zinc-oxide-nanoparticle-intoxicated rats following treatment with intragastric quercetin (200 mg/kg) for three weeks [44]. Osteolysis is defined by pathological destruction or disappearance of bone tissues.…”

Oral Administration of Quercetin or Its Derivatives Inhibit Bone Loss in Animal Model of Osteoporosis

“…A total of 126 studies were identified by the initial database search and 19 eligible studies [4,27,[30][31][32][33][34][35][36][37][38][39][40][41][42][43][44][45][46] containing 21 comparisons were included in this study ultimately. The process followed for study selection is shown in Figure 2.…”