Allgemeine mikroskopische Anatomie der lebenden Masse

Hertwig, Günther

doi:10.1007/978-3-642-81037-4_1

Cited by 41 publications

(3 citation statements)

References 366 publications

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“…The demonstration of myosin and prekeratin in the same cells of various organs confirms data by Heidenhain (1899, 191l), Hertwig (1929), Studni~ka (1929) and others that many epithelial cells contain contractile myofibrils as well as tonofibrils that function as a cytoskeleton and provide resistance to mechanical stress, e.g. cell deformation.…”

Section: Discussionsupporting

confidence: 84%

A comparative study of myosins and prekeratin in epithelial cells of methacarn-fixed tissues

et al. 1985

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Around the turn of the century, tonofibrils and contractile myofibrils were observed within the same cells. These findings have been largely forgotten. To clarify the topical relations of these proteins in epithelial cells, duplicate sections of methacarn-fixed human and canine tissues were treated with the tannic acid-phosphomolybdic acid (TP)-Levanol Fast Cyanine 5RN reaction for myosins and the PAP technic for prekeratin, respectively. In bronchi, lingual and sweat glands, liver and pancreas, myosin was confined to the terminal bar-terminal web system, including pericanalicular layers. Prekeratin occurred throughout the epithelium of bronchi and ducts; secretory cells showed little or no reaction. Observations on myosin in kidney confirmed data by Harper et al. (1970). The PAP technic colored transitional epithelium and collecting tubules intensely; convoluted tubules did not react. Staining of segments of Henle's loops varied from case to case. Both reactions colored thymic epithelial cells. In myoid cells of Hassall's corpuscles myosin was gradually replaced by prekeratin and keratin. Basal cells of epididymis reacted strongly with the PAP technic, but did not contain myosin. Prekeratin is apparently identical with epidermin, whose composition and structure were well known in the 1950's. Epidermin undergoes chemical changes as cells move from the stratum basale to the stratum corneum. According to DAKO, the antibodies used in this study were prepared with prekeratin extracted from stratum corneum. Data in the literature and observations in this investigation indicate that some samples of antibodies do not react with all tonofilaments.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Discussionsupporting

confidence: 84%

A comparative study of myosins and prekeratin in epithelial cells of methacarn-fixed tissues

et al. 1985

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“…Such a conclusion is in harmony with "classical" cytology, suggesting that the chromosome continuity in the mitotic division and interphase in which each chromatin region -"Kernbezirk" -actually represents a chromosomal territory (see e.g. Hertwig 1929, Wassermann 1929, Pederson 2004). The increased width of chromosomes in the metaphase is not surprising because of known chromatid-splitting steps in measured cells.…”

Section: Discussionmentioning

confidence: 58%

“…The comparison of the central heterochromatin fibril or chromosome width measured in the present study with various measurement data or models of chromosome or chromatin fibrils would be difficult. The width of both chromosomes and chromatin fibrils is influenced by methods used for specimen preparation and microscopic measurements including evaluation, as has been known since the first part of the last century (Hertwig 1929, Wassermann 1929. Nevertheless, in the present study the width similarity of chromosomes and central heterochromatin fibrils was noted in the same specimens using the same measurement and evaluation procedures.…”

Section: Discussionmentioning

confidence: 99%

The DNA chromatin condensation expressed by the image optical density of chromosomes and heterochromatin in proliferating single human leukemic granulocytic progenitors

et al. 2012

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The appearance of heterochromatin is generally accepted as a useful tool for the evaluation of the cell state including pathology; however, information on the heterochromatin DNA condensation state expressed by the image optical density in interphase nuclear regions and mitotic chromosomes with silent genes is very limited. Since human proliferating myeloblasts are a very convenient model, they were studied in the bone marrow of leukemic patients and established cell cultures using computer assisted image densitometry at the single cell level after heterochromatin visualization by a simple but sensitive cytochemical procedure for demonstration of DNA. As was expected, a high DNA image optical density was noted in central heterochromatin regions in contrast to the nuclear periphery at the nuclear envelope. Similarly, a high nuclear DNA image optical density was also expressed in mitotic chromosomes. Thus the possibility exists that the large heterochromatin DNA condensation expressed by the large image optical density in central nuclear regions, as in mitotic chromosomes, is related to silent gene locations. The similar width of mitotic chromosomes and chromatin fibrils in the heterochromatin regions in the interphase nuclei supports that explanation. The chromatin DNA fibrils in the central heterochromatin nuclear regions of interphase cells might just represent masked silent chromosomal segments. Such a conclusion is in harmony with "classical" cytology in the first part of the last century, which suggests the chromosome continuity from the mitotic division to the interphase where each chromatin region ("Kernbezirk") actually represents a chromosomal territory.

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Allgemeine morphologische Pathologie des Cytoplasmas. Die Pathobiosen

Altmann

1955

Das Cytoplasma

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Allgemeine mikroskopische Anatomie der lebenden Masse

Cited by 41 publications

References 366 publications

A comparative study of myosins and prekeratin in epithelial cells of methacarn-fixed tissues

A comparative study of myosins and prekeratin in epithelial cells of methacarn-fixed tissues

The DNA chromatin condensation expressed by the image optical density of chromosomes and heterochromatin in proliferating single human leukemic granulocytic progenitors

Allgemeine morphologische Pathologie des Cytoplasmas. Die Pathobiosen

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