Allium Genetic Resources

Keller, E. R.; Kik, C.

doi:10.1007/978-3-319-95825-5_3

Cited by 8 publications

(6 citation statements)

References 102 publications

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“…Advantages of in vitro onion collections include compact storage, diminished space requirements, and the capacity for rapid multiplication and distribution of plant materials, in particular vegetative propagated materials [ 80 ]. This method proves particularly beneficial for conserving onion varieties that are rare or endangered and Allium species that pose challenges to preservation through conventional seed storage techniques [ 81 ].…”

Section: Ex Situ Conservation Efforts For Onion Genetic Resourcesmentioning

confidence: 99%

“…For Alliums, which do not mainly reproduce by seed, cryopreservation is the most convenient strategy for their conservation [ 84 ]. In onions, this process allows for the preservation of various tissues, such as embryonic axes, shoot tips, or dormant bulbs, using cryoprotectants (Vitrification)to shield them from cellular damage and ice crystal formation [ 81 , 85 , 86 ]. Cryopreservation enables the storage of germplasm for extended periods, potentially decades or even centuries, whilst maintaining the genetic integrity and viability of the stored materials [ 81 , 87 ].…”

Section: Ex Situ Conservation Efforts For Onion Genetic Resourcesmentioning

confidence: 99%

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Conservation and Global Distribution of Onion (Allium cepa L.) Germplasm for Agricultural Sustainability

Ochar,

Kim

2023

Plants

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Onion (Allium cepa L.) is recognized globally as a crucial vegetable crop, prized not only for its culinary applications but also for its numerous health-promoting properties. With climate change relentlessly exerting mounting challenges to agriculture, the preservation and deployment of onion germplasm has become critical to ensuring sustainable agriculture and safeguarding food security. Global onion germplasm collections function as repositories of genetic diversity, holding within them an extensive array of valuable traits or genes. These can be harnessed to develop varieties resilient to climate adversities. Therefore, detailed information concerning onion germplasm collections from various geographical regions can bolster their utility. Furthermore, an amplified understanding of the importance of fostering international and inter-institutional collaborations becomes essential. Sharing and making use of onion genetic resources can provide viable solutions to the looming agricultural challenges of the future. In this review, we have discussed the preservation and worldwide distribution of onion germplasm, along with its implications for agricultural sustainability. We have also underscored the importance of international and interinstitutional collaboration in onion germplasm collecting and conservation for agricultural sustainability.

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Section: Ex Situ Conservation Efforts For Onion Genetic Resourcesmentioning

confidence: 99%

Section: Ex Situ Conservation Efforts For Onion Genetic Resourcesmentioning

confidence: 99%

Conservation and Global Distribution of Onion (Allium cepa L.) Germplasm for Agricultural Sustainability

Ochar,

Kim

2023

Plants

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“…aggregatum), (ii) the presence of heterozygous seeds, i.e., in onion (Allium cepa var. cepa), or (iii) the traditional breeding strategy as clonal varieties as in the case of some ornamental Allium hybrids [20].…”

Section: Management Of Field Genebanksmentioning

confidence: 99%

Challenges and Prospects for the Conservation of Crop Genetic Resources in Field Genebanks, in In Vitro Collections and/or in Liquid Nitrogen

Panis

Nagel

houwe

2020

Plants

109

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The conservation of crop genetic resources, including their wild relatives, is of utmost importance for the future of mankind. Most crops produce orthodox seeds and can, therefore, be stored in seed genebanks. However, this is not an option for crops and species that produce recalcitrant (non-storable) seeds such as cacao, coffee and avocado, for crops that do not produce seeds at all; therefore, they are inevitably vegetatively propagated such as bananas, or crops that are predominantly clonally propagated as their seeds are not true to type, such as potato, cassava and many fruit trees. Field, in vitro and cryopreserved collections provide an alternative in such cases. In this paper, an overview is given on how to manage and setup a field, in vitro and cryopreserved collections, as well as advantages and associated problems taking into account the practical, financial and safety issues in the long-term. In addition, the need for identification of unique accessions and elimination of duplicates is discussed. The different conservation methods are illustrated with practical examples and experiences from national and international genebanks. Finally, the importance of establishing safe and long-term conservation methods and associated backup possibilities is highlighted in the frame of the global COVID-19 pandemic.

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“…Bawang merah termasuk ke dalam genus Allium, subfamilia Allioideae (Amaryllidaceae) (APG 2016), merupakan salah satu genus terbesar dari kelompok monokotil yaitu ~920 spesies, dan merupakan tanaman yang memiliki nilai ekonomi yang penting di dunia pertanian (Herden et al 2016, Shigyo et al 2018. Tanaman bawang merah umumnya diploid dengan jumlah kromosom 16 (2n = 2 = 16) (Firbas and Amon 2014).…”

Section: Pendahuluanunclassified

IDENTIFIKASI KROMOSOM HOMOLOG MELALUI DETEKSI NUCLEOLUS ORGANIZER REGIONS DENGAN PEWARNAAN AgNO3 PADA TANAMAN BAWANG MERAH - IN PRESS -

Puspita

Setiawan²,

Purwantoro³

et al. 2020

J Bioteknol Biosains Indones

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Generally, the standard procedure for karyotype analysis of shallot is sorted by chromosome sizes. Therefore, the identification of homologous chromosomes is difficult without using a specific probe. Nucleolus Organizing Regions (NORs) can be used as a probe for precise identification of homologous chromosomes. However, the use of NORs for plant karyotyping in Indonesia is poorly investigated. In this study, shallot chromosomes were prepared using modified Carnoy’s solution II, fixed in Carnoy’s solution, and stained by using aceto-carmine and AgNO3 for detecting NORs. Chromosome images were analyzed by CHIAS IV. One locus NOR bearing chromosome pair was detected at metaphase and interphase, and it was located at short arms of subtelomeric chromosome number 6. NORs can be used as a probe for precise identification of homologous chromosomes in shallot. Therefore, this technique has the potential to be applied on species closely related to shallot and on other plant species.Keywords: AgNO3, chromosome condensation, NORs, shallot chromosome, shallot karyotype ABSTRAKProsedur kariotipe untuk bawang merah umumnya masih disusun berdasarkan ukuran kromosom, sehingga diperlukan suatu penanda yang dapat mengidentifikasi kromosom homolog secara presisi. Identifikasi kromosom homolog secara presisi menggunakan suatu penanda, khususnya deteksi Nucleolus Organizing Regions (NORs), yang di Indonesia masih jarang dilakukan. Penelitian ini bertujuan untuk membuat kariotipe dan mengidentifikasi kromosom homolog bawang merah melalui deteksi NORs menggunakan metode pewarnaan AgNO3. Proses fiksasi akar dilakukan dengan menggunakan modifikasi larutan Carnoy II, lalu difiksasi dengan larutan Carnoy, dan kromosom diwarnai dengan aceto-carmine dan larutan AgNO3 untuk mendeteksi NORs. Selanjutnya, citra kromosom dianalisis menggunakan CHIAS IV. Hasil penelitian menunjukkan bahwa terdapat sepasang NORs yang terdeteksi pada fase metafase dan interfase yang terletak pada bagian lengan pendek di kromosom subtelosentrik nomor 6. Hasil dari penelitian ini dapat dijadikan sebagai dasar di bidang sitogenetika bawang merah untuk mengidentifikasi kromosom homolog secara presisi menggunakan penanda NOR. Oleh karenanya, teknik ini dapat diaplikasikan pada spesies yang berdekatan dengan bawang merah dan komoditas tanaman lainnya.Kata Kunci: AgNO3, kariotipe bawang, kondensasi kromosom, kromosom bawang, NORs

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Allium Genetic Resources

Cited by 8 publications

References 102 publications

Conservation and Global Distribution of Onion (Allium cepa L.) Germplasm for Agricultural Sustainability

Conservation and Global Distribution of Onion (Allium cepa L.) Germplasm for Agricultural Sustainability

Challenges and Prospects for the Conservation of Crop Genetic Resources in Field Genebanks, in In Vitro Collections and/or in Liquid Nitrogen

IDENTIFIKASI KROMOSOM HOMOLOG MELALUI DETEKSI <em>NUCLEOLUS ORGANIZER REGIONS</em> DENGAN PEWARNAAN AgNO3 PADA TANAMAN BAWANG MERAH <strong><em>- IN PRESS -</em></strong>

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