Allocation of Fe and ferric chelate reductase activities in mesophyll cells of barley and sorghum under Fe-deficient conditions

Mikami, Yuichiro; Saito, Akihiro; Miwa, Eitaro; Higuchi, Kyoko

doi:10.1016/j.plaphy.2011.01.009

Cited by 24 publications

(23 citation statements)

References 34 publications

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“…Indeed, the restoration of some Fe-requiring physiological parameters indicates that the re-distribution of Fe in the mesophyll cells plays an important role, which process also involves the allocation of Fe into the chloroplasts ( Figure 1B, [39]). Fe acquisition by chloroplasts can also be monitored by the slow increase in the Chl content [40] and by the recovery of photochemical activity.…”

Section: Metal Re-distribution and Hardeningmentioning

confidence: 99%

Stress hardening under long-term cadmium treatment is correlated with the activation of antioxidative defence and iron acquisition of chloroplasts in Populus

Solti

Sárvári

Szöllősi

et al. 2016

Zeitschrift Für Naturforschung C

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Cadmium (Cd), a highly toxic heavy metal affects growth and metabolic pathways in plants, including photosynthesis. Though Cd is a transition metal with no redox capacity, it generates reactive oxygen species (ROS) indirectly and causes oxidative stress. Nevertheless, the mechanisms involved in long-term Cd tolerance of poplar, candidate for Cd phytoremediation, are not well known. Hydroponically cultured poplar (Populus jacquemontiana var. glauca cv. 'Kopeczkii') plants were treated with 10 μM Cd for 4 weeks. Following a period of functional decline, the plants performed acclimation to the Cd induced oxidative stress as indicated by the decreased leaf malondialdehyde (MDA) content and the recovery of most photosynthetic parameters. The increased activity of peroxidases (PODs) could have a great impact on the elimination of hydrogen peroxide, and thus the recovery of photosynthesis, while the function of superoxide dismutase (SOD) isoforms seemed to be less important. Re-distribution of the iron content of leaf mesophyll cells into the chloroplasts contributed to the biosynthesis of the photosynthetic apparatus and some antioxidative enzymes. The delayed increase in photosynthetic activity in relation to the decline in the level of lipid peroxidation indicates that elimination of oxidative stress damage by acclimation mechanisms is required for the restoration of the photosynthetic apparatus during long-term Cd treatment.

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Section: Metal Re-distribution and Hardeningmentioning

confidence: 99%

Stress hardening under long-term cadmium treatment is correlated with the activation of antioxidative defence and iron acquisition of chloroplasts in Populus

Solti

Sárvári

Szöllősi

et al. 2016

Zeitschrift Für Naturforschung C

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“…Interestingly, it has recently reported the presence and expression of FRO genes in grasses such as barley and sorghum (Mikami et al, 2011), which raises the question of whether some other species of agricultural interest also present functional FRO genes, thus opening the possibility of improving their capabilities for iron acquisition. To date, no FRO activity has been observed in roots; this activity has only been detected in shoots and specific cellular organelles, thus likely contributing to Fe homeostasis and photosynthetic capacity (Mikami et al, 2011).…”

mentioning

confidence: 99%

“…To date, no FRO activity has been observed in roots; this activity has only been detected in shoots and specific cellular organelles, thus likely contributing to Fe homeostasis and photosynthetic capacity (Mikami et al, 2011).…”

mentioning

confidence: 99%

“…It has been argued that O. sativa (Os)FRO2 and OsFRO1 may not code for ferric chelate reductase functions, even when these activities have been reported only in mesophyll cells in other plant grasses such as barley and sorghum (Mikami et al, 2011). Interestingly, the expression of FRO1 and FRO2 genes in barley and sorghum was detected in leaves but not in roots.…”

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confidence: 99%

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Identification and expression analysis of multiple FRO gene copies in Medicago truncatula

et al. 2012

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ABSTRACT. Iron (Fe) is an essential element for plant growth. Commonly, this element is found in an oxidized form in soil, which is poorly available for plants. Therefore, plants have evolved ferric-chelate reductase enzymes (FRO) to reduce iron into a more soluble ferrous form. Fe scarcity in plants induce the FRO enzyme activity. Although the legume Medicago truncatula has been employed as a model for FRO activity studies, only one copy of the M. truncatula MtFRO1 gene has been characterized so far. In this study, we identified multiple gene copies of the MtFRO gene in the genome of M. truncatula by an in silico search, using BLAST analysis in the database of the M. truncatula Genome Sequencing Project and the National Center for Biotechnology Information, and also determined whether they are functional. We identified five genes apart from MtFRO1, which had been already characterized. All of the MtFRO genes exhibited high identity with homologous FRO genes from Lycopersicon esculentum, Citrus junos and Arabidopsis thaliana. The gene copies also presented characteristic conserved FAD and NADPH motifs, transmembrane regions and oxidoreductase signature motifs. We also detected expression in five of the putative MtFRO sequences by semiquantitative RT-PCR analysis,

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“…Further, Mikami et al (2011) reported that photosynthetic apparatus need a large amount of iron and among the available Fe, about 60% of the iron content of Citrus lemon leaf is in the thylakoid; hence, the lack of iron directly affect the amount of chlorophyll produced and will ultimately lead to chlorosis (Hellin et al,1995). Tolerant plants may allocate iron more efficiently to its thylakoids under Fedeficient conditions than susceptible plants (Mikami et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

In-vitro analysis of the efficacy of Fe oxide nanoparticles in prevention of iron deficiency chlorosis in citrus rootstock (Citrus volkameriana)

Saeedi¹,

Mousavi²,

Mogharab³

2016

JEBAS

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KEYWORDS Fe oxide nanoparticles Iron deficiency Chlorosis in vitro Citrus volkameriana ABSTRACTPresent study was undertaken to explore of the effect iron nanoparticles (uncoated iron oxide nanoparticles, iron oxide nanoparticles coated with polyethylene glycol and FeEDTA and FeEDDHA chelates) on prevention of chlorosis in Citrus volkameriana. Study was carried out under the in vitro condition on MS medium supplemented with different iron compositions. Various growth attributes such as chlorophyll a, b, iron content of leaves, root and shoot length, number and area of leaves, degree of chlorosis, dry and fresh weights of seedling, roots and shoots were measured in end of study. Results of study revealed that seedlings grown on MS culture medium supplemented with uncoated nanoparticles of iron oxide had the highest rates of iron uptake and the lowest degree of chlorosis. Further, plant cultured on the MS medium with uncoated iron nanoparticles significantly increased vegetative growth characteristics such as, fresh and dry weights and chlorophyll content over the control (MS without chelated iron). Plant cultured on MS medium supplemented with uncoated iron oxide nanoparticles showed superiority over the plant cultured on MS media without iron and improved leaves growth characteristics and decreased chlorosis.

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Allocation of Fe and ferric chelate reductase activities in mesophyll cells of barley and sorghum under Fe-deficient conditions

Cited by 24 publications

References 34 publications

Stress hardening under long-term cadmium treatment is correlated with the activation of antioxidative defence and iron acquisition of chloroplasts in Populus

Stress hardening under long-term cadmium treatment is correlated with the activation of antioxidative defence and iron acquisition of chloroplasts in Populus

Identification and expression analysis of multiple FRO gene copies in Medicago truncatula

In-vitro analysis of the efficacy of Fe oxide nanoparticles in prevention of iron deficiency chlorosis in citrus rootstock (Citrus volkameriana)

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