Allogenic Use of Human Placenta-Derived Stromal Cells as a Highly Active Subtype of Mesenchymal Stromal Cells for Cell-Based Therapies

Gorodetsky, Raphael; Aicher, Wilhelm K.

doi:10.3390/ijms22105302

Cited by 23 publications

(32 citation statements)

References 251 publications

(352 reference statements)

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“…SUI cell therapies may support self-healing of the urethral sphincter muscle by activating local satellite cells 7 , by improving vascularization and reducing inflammation 41 , 42 , or by complementing the deficient sphincter muscle by myogenic cells 5 , 13 – 15 , 43 , 44 . But these processes may take some time.…”

Section: Discussionmentioning

confidence: 99%

Replacing Needle Injection by a Novel Waterjet Technology Grants Improved Muscle Cell Delivery in Target Tissues

et al. 2022

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Current regimen to treat patients suffering from stress urinary incontinence often seems not to yield satisfactory improvement or may come with severe side effects. To overcome these hurdles, preclinical studies and clinical feasibility studies explored the potential of cell therapies successfully and raised high hopes for better outcome. However, other studies were rather disappointing. We therefore developed a novel cell injection technology to deliver viable cells in the urethral sphincter complex by waterjet instead of using injection needles. We hypothesized that the risk of tissue injury and loss of cells could be reduced by a needle-free injection technology. Muscle-derived cells were obtained from young male piglets and characterized. Upon expansion and fluorescent labeling, cells were injected into cadaveric tissue samples by either waterjet or injection needle. In other experiments, labeled cells were injected by waterjet in the urethra of living pigs and incubated for up to 7 days of follow-up. The analyses documented that the cells injected by waterjet in vitro were viable and proliferated well. Upon injection in live animals, cells appeared undamaged, showed defined cellular somata with distinct nuclei, and contained intact chromosomal DNA. Most importantly, by in vivo waterjet injections, a significantly wider cell distribution was observed when compared with needle injections ( P < .05, n ≥ 12 samples). The success rates of waterjet cell application in living animals were significantly higher (≥95%, n = 24) when compared with needle injections, and the injection depth of cells in the urethra could be adapted to the need by adjusting waterjet pressures. We conclude that the novel waterjet technology injects viable muscle cells in tissues at distinct and predetermined depth depending on the injection pressure employed. After waterjet injection, loss of cells by full penetration or injury of the tissue targeted was reduced significantly in comparison with our previous studies employing needle injections.

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Section: Discussionmentioning

confidence: 99%

Replacing Needle Injection by a Novel Waterjet Technology Grants Improved Muscle Cell Delivery in Target Tissues

et al. 2022

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“…Cells injected into the target tissue in situ support tissue regeneration for a short period. MSCs, for instance, release factors facilitating tissue regeneration and vascularization such as fibroblast growth factor (FGF) and vascular endothelial growth factor (VEGF), playing an important role in wound healing and neoangiogenesis facilitating better wound healing [55,56]. When cells are injected in combination with biomaterials, the biomaterial may even create a physical barrier avoiding loss of cells by efflux, entry, e.g., of urine or bacteria into the wound and prevents bleeding after injection.…”

Section: Discussionmentioning

confidence: 99%

Novel Techniques to Improve Precise Cell Injection

Linzenbold

Fech

Hofmann

et al. 2021

IJMS

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We noted recently that the injection of cells with a needle through a cystoscope in the urethral sphincter muscle of pigs failed to deposit them nearby or at the intended target position in about 50% of all animals investigated (n > 100). Increasing the chance for precise cell injection by shotgun approaches employing several circumferential injections into the sphincter muscle bears the risk of tissue injury. In this study, we developed and tested a novel needle-free technique to precisely inject cells in the urethral sphincter tissue, or other tissues, using a water-jet system. This system was designed to fit in the working channels of endoscopes and cystoscopes, allowing a wide range of minimally invasive applications. We analyze key features, including the physical parameters of the injector design, pressure ranges applicable for tissue penetration and cell injections and biochemical parameters, such as different compositions of injection media. Our results present settings that enable the high viability of cells post-injection. Lastly, the method is suitable to inject cells in the superficial tissue layer and in deeper layers, required when the submucosa or the sphincter muscle of the urethra is targeted.

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“…However, depending on the tissue, such as the brain or heart, it is difficult to separate stem cells from the living tissue and for use as treatment. In recent years, MSCs have been the focus of attention because they are relatively easy to extract from various tissues, including the bone marrow, adipose tissue, placenta, umbilical cord, synovium, and pulp [ 146 , 147 , 148 , 149 , 150 ]. Furthermore, they can also differentiate into ectoderm-derived nerve cells and glial cells that perform functions such as supporting nerve cells and endoderm-derived hepatocytes [ 151 , 152 , 153 , 154 ].…”

Section: Mesenchymal Stem Cells (Mscs) For Regenerationmentioning

confidence: 99%

Therapeutic Strategy of Mesenchymal-Stem-Cell-Derived Extracellular Vesicles as Regenerative Medicine

Matsuzaka

Yashiro

2022

IJMS

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Extracellular vesicles (EVs) are lipid bilayer membrane particles that play critical roles in intracellular communication through EV-encapsulated informative content, including proteins, lipids, and nucleic acids. Mesenchymal stem cells (MSCs) are pluripotent stem cells with self-renewal ability derived from bone marrow, fat, umbilical cord, menstruation blood, pulp, etc., which they use to induce tissue regeneration by their direct recruitment into injured tissues, including the heart, liver, lung, kidney, etc., or secreting factors, such as vascular endothelial growth factor or insulin-like growth factor. Recently, MSC-derived EVs have been shown to have regenerative effects against various diseases, partially due to the post-transcriptional regulation of target genes by miRNAs. Furthermore, EVs have garnered attention as novel drug delivery systems, because they can specially encapsulate various target molecules. In this review, we summarize the regenerative effects and molecular mechanisms of MSC-derived EVs.

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Allogenic Use of Human Placenta-Derived Stromal Cells as a Highly Active Subtype of Mesenchymal Stromal Cells for Cell-Based Therapies

Cited by 23 publications

References 251 publications

Replacing Needle Injection by a Novel Waterjet Technology Grants Improved Muscle Cell Delivery in Target Tissues

Replacing Needle Injection by a Novel Waterjet Technology Grants Improved Muscle Cell Delivery in Target Tissues

Novel Techniques to Improve Precise Cell Injection

Therapeutic Strategy of Mesenchymal-Stem-Cell-Derived Extracellular Vesicles as Regenerative Medicine

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