Allosteric aptasensor-initiated target cycling and transcription amplification of light-up RNA aptamer for sensitive detection of protein

Aptamer-based kinetically controlled DNA reactions coupled with metal–organic framework nanoprobes for sensitive detection of SARS-CoV-2 spike protein

“…As shown in Table S2, † the method had better performance in terms of both detection limit and linear range. [15][16][17][18][19][20] At the same time, the method used easily accessed materials and required no additional design of the aptamer segment, so it could be easily extended to the detection of other protein markers by simply changing the aptamer segment. Of course, the method also had some limitations.…”

“…Emerging aptamers with good binding affinity and speci-city provide opportunities for the establishment of detection methods for SARS-CoV-2 spike protein. [13][14][15][16][17][18][19][20] For example, Metternich et al made use of two aptamers targeting SARS-CoV-2 spike protein to develop a uorescence detection method based on covalent guanine quantum defect chemistry; 15 Luo et al demonstrated the usability of aptamer-functionalized MXene nanosheets as a novel sensor that allowed detection of SARS-CoV-2 spike protein down to 38.9 fg mL −1 ; 17 Xue et al reported an electrochemical method based on aptamer-binding induced multiple hairpin assembly and successfully applied it to detect as low as 9.79 fg mL −1 SARS-CoV-2 spike protein. 20 Although these methods have shown promising feasibility, they still have some limitations.…”

Investigating the Effect of RNA Scaffolds on the Multicolor Fluorogenic Aptamer Pepper in Different Bacterial Species

“…A notable feature of the Pepper aptamer is that it can bind to eight different HBC dye analogs to give fluorescence emission across the visible spectrum, ranging from cyan to red . Although some studies have demonstrated the robustness of this aptamer in vitro and in eukaryotic cells, − its activity in different bacteria remains largely unexplored. Only recently, a series of Pepper-based biosensors have been tested in E. coli .…”

Aptamer-functionalized two-photon SiO2@GQDs hybrid-based signal amplification strategy for targeted cancer imaging

“…2,3 Up to now, antibodies, 4 folic acid, [5][6][7][8] peptides, 9,10 and lectins 11 have emerged as important elements for the recognition of cancer cells. In particular, aptamers, as short, single-stranded oligonucleotides generated by SELEX, can specifically bind to various targets (such as small molecules, 12 proteins, 13 biological cells, 14 and even tissues 15 ) with high affinity and selectivity. In addition, aptamers often termed "chemical antibodies", can not only recognize their targets with high specificity comparable to that of antibodies, but also possess several unique advantages, such as easy synthesis and modification, long-term stability, nontoxicity, and lack of immunogenicity.…”