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Abstract

Four lambda clones (lambda G11, lambda C4, lambda G14 and lambda G17) from the porcine MHC class III region were labelled with either biotin-14-dATP or digoxigenin-11-dUTP and hybridized in two different combinations to DNA fibres. The latter were prepared from agarose-embedded porcine peripheral lymphocytes lysed with proteinase K, then spread and fixed on poly-L-lysine-coated slides. Hybridization signals thus obtained confirm the order of the clones previously reported using pulsed-field gel electrophoresis (PFGE). Measurements of probe sizes, gap distances between probes and total length of DNA encompassing the probes were made. Three different methods, namely relative length, Watson-Crick standard and probe size standard-based conversions, were used to estimate the parameters in kilobases. These methods of data conversion were compared with each other and with the available PFGE data, and their utility and accuracy were evaluated.

Modeling description and spectroscopic evidence of surface acid–base properties of natural illites

“…In this study, 2.7470.023 was adopted for pK f1 , which was estimated based on potentiometric titration (Sjo¨berg, 1998). Meanwhile, since the used FITEQL cannot fit the bulk deposition observed in the acidic supernatant during the hydroxide titration, a supposed reaction to simulate the interactions between aluminum species and silicic acid during the hydroxide titration was further introduced, where K f2 represents the formation constant for the produced complex.…”

A detailed physical map of the horse Y chromosome

“…Peripheral blood from a male horse (Reg #1219, Texas A&M University) with known fertility was used for (i) standard short-term pokeweed (Sigma) stimulated lymphocyte cultures to obtain interphase and metaphase chromosome preparations and (ii) agarose-embedded DNA plugs to obtain DNA fibers (31,32). DNA from individual BAC clones was labeled with biotin-16-dUTP and͞or digoxigenin-11-dUTP by nick translation using Biotin-or DIG-Nick Translation Mix (Roche Molecular Biochemicals).…”

“…DNA from individual BAC clones was labeled with biotin-16-dUTP and͞or digoxigenin-11-dUTP by nick translation using Biotin-or DIG-Nick Translation Mix (Roche Molecular Biochemicals). Hybridization to metaphase chromosomes or mechanically stretched DNA fibers was performed as described elsewhere in detail by us (30,(32)(33)(34). A minimum of 30 metaphase spreads and Ϸ50 interphase cells or DNA fiber hybridizations were captured and analyzed for each experiment by using a Zeiss Axioplan2 fluorescent microscope equipped with CYTOVISION͞GENUS application software, Version 2.7 (Applied Imaging, Santa Clara, CA).…”

Kinetic inhibition of calcite (104) dissolution by aqueous manganese(II)

“…[4,6,[18][19][20]) has received much less attention compared to that directed at their influence on growth (e.g., Refs. [2,3,5,8,[15][16][17][21][22][23]).…”