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Sayed, Haitham; Kayyal, Hamed; Ramsey, Luke; Ceccarelli, Salvatore; Baum, Michaël

doi:10.1023/a:1015861610226

Cited by 36 publications

(5 citation statements)

References 31 publications

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“…The data were further filtered to retain markers with an amplification rate of >80% and to exclude co-segregating markers. Markers showing segregation distortion were excluded in JoinMap Version 5 (Van Ooijen, 2018), unless clustered together with the correct Morex genome assembly order, under the assumption of biological significance which is common in wide crosses (Zamir & Tadmor, 1986), or that they are linked to traits affecting regeneration of plantlets following microspore-culture (Sayed et al, 2002). Markers were grouped into linkage groups by their independence LOD in JoinMap Version 5 and ordered within each linkage group by the maximum likelihood algorithm with the default parameters.…”

Section: Genetic Map Construction and Genetic Mapping Of Barley Powdery Mildew Resistance Locimentioning

confidence: 99%

Adult resistance genes to barley powdery mildew confer basal penetration resistance associated with broad‐spectrum resistance

Wentzel

D'Souza

et al. 2021

The Plant Genome

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Powdery mildew isa major disease of barley (Hordeum vulgare L.) for which breeders have traditionally relied on dominant, pathogen race-specific resistance genes for genetic control. Directional selection pressures in extensive monocultures invariably result in such genes being overcome as the pathogen mutates to evade recognition. This has led to a widespread reliance on fungicides and a single broad-spectrum recessive resistance provided by the mlo gene. The range of resistance genes and alleles found in wild crop relatives and landraces has been reduced in agricultural cultivars through an erosion of genetic diversity during domestication and selective breeding. Three novel major-effect adult plant resistance (APR) genes from landraces, designated Resistance to Blumeria graminis f. sp. hordei (Rbgh1 to Rbgh3), were identified in the terminal regions of barley chromosomes 5HL, 7HS, and 1HS, respectively. The phenotype of the new APR genes showed neither pronounced penetration resistance, nor the spontaneous necrosis and mesophyll cell death typical of mlo resistance, nor a whole epidermal cell hypersensitive response, typical of racespecific resistance. Instead, resistance was localized to the site of attempted penetration in an epidermal cell and was associated with cell wall appositions and cytosolic vesicle-like bodies, and lacked strong induction of reactive oxygen species. The APR genes exhibited differences in vesicle-like body sizes, their distribution, and the extent of localized 3,3-diaminobenzidine staining in individual doubled haploid lines. The results revealed a set of unique basal penetration resistance genes that offer opportunities for combining different resistance mechanisms in breeding programs for robust mildew resistance.

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Section: Genetic Map Construction and Genetic Mapping Of Barley Powdery Mildew Resistance Locimentioning

confidence: 99%

Adult resistance genes to barley powdery mildew confer basal penetration resistance associated with broad‐spectrum resistance

Wentzel

D'Souza

et al. 2021

The Plant Genome

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“…The usual way to assess segregation distortion for marker data is by χ 2 tests (Haitham et al, 2002;Lu et al, 2002;Ruiz and Asins, 2003). In the current study, Ninety three (82.3%) primers showed 1:1 disomic segregation pattern and twenty marker alleles (17.69%) showed deviations from Mendelian segregation pattern.…”

Section: Construction Of Genetic Linkage Map and Qtl Mappingmentioning

confidence: 58%

Construction of SSR Based Genetic Linkage Map and Identification of QTLs Conferring CMD Resistance in Cassava

Paikaray¹,

Nair²,

Mohan³

et al. 2020

IJBSM

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Cassava (Manihot esculenta) belonging to the family Euphorbiaceae, is one of the most important tuber crops cultivated extensively in tropical and subtropical regions of Africa and Asia. It is a cross pollinating crop with a diploid number of chromosome 2n=36 and DNA content of 1.67pg per cell. It is highly heterozygous, monoecious and protogynous in nature. The tuberous crop is propagated vegetatively by means of stem cuttings and sexually through seeds. According to Food and Agriculture Organization Corporate Statistical Database (FAOSTAT, 2017), the global cassava production was estimated to be 291992646 tonnes in which 61% is accounted by Africa, 29.3% contributed by Asia and remaining 9.6% by America. Indian cassava production was accounted to be 42 mt from an area of 199 mha with a productivity of 2,09,598 hg ha-1. Cassava is mainly used as food and also as an important raw material for various industrial purposes. The wide acceptability of cassava in the world is increasing due to the broad agro-ecological adaptability, its ability to mitigate unfavourable environmental condition and its abilities to produce reasonable yield. Cassava production is always challenged Article History

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“…Nonetheless as noticed earlier, in the present study, some of the number of positives generated from the BC 1 F 1 cross from CSIR/CRI-ATS06 deviated from the typical Mendelian pattern of segregation, probably due to selfed seeds, which might have resulted in fewer number of positive plants in that backcross. According to Sayed et al (2002) even though markers will generally segregate in a Mendelian manner, distorted segregation ratios may be encountered. Identification of positives for alc gene in BC 1 F 1 generation through phenotypic screening is very difficult and is time consuming.…”

Section: Discussionmentioning

confidence: 99%

Marker assisted backcrossing of alcobaca gene into two elite tomato breeding lines

Osei¹,

Danquah²,

Adu-Dapaah³

et al. 2022

Front. Hortic.

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Tomato is one of the most important vegetable crops cultivated and consumed worldwide. Demand is increasing daily because of increase in per capita fresh fruit consumption. Despite the economic importance of tomato, it has relatively short shelf-life after ripening and experiences remarkable post-harvest losses. This study was aimed at using marker-assisted backcrossing (MABC) to transfer shelf-life gene (alc) into the genetic background of two elite Ghanaian tomato breeding lines. The MABC derived lines at BC2F3 were evaluated to confirm selections using molecular markers. All the MABC-derived lines gave significant extended shelf life compared to the checks except for Alc-LA3134, the alc donor parent, which was, however, not significantly different from one of the backcrosses (BC2F2.3-E-80-19-26). This confirms that the genetic regulation of the shelf-life trait is dependent on the alc gene. The performance of the donor check (Alc-LA3134) against the 12 MABC-derived lines indicated no significant differences for the fruit firmness (except for BC2F2.3-E-80-19-4), number of locules per fruit and shelf life (for only BC2F2.3-E-80-19-26), thus suggesting similar backgrounds of the MABC as the recurrent parents. A considerable increase in the shelf-life value was observed among the MABC-derived lines. The introgression of the alc gene into cultivated tomatoes for extended shelf-life could reduce post-harvest losses of tomato in Ghana.

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Untitled

Cited by 36 publications

References 31 publications

Adult resistance genes to barley powdery mildew confer basal penetration resistance associated with broad‐spectrum resistance

Adult resistance genes to barley powdery mildew confer basal penetration resistance associated with broad‐spectrum resistance

Construction of SSR Based Genetic Linkage Map and Identification of QTLs Conferring CMD Resistance in Cassava

Marker assisted backcrossing of alcobaca gene into two elite tomato breeding lines

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