Alpha-mangostin suppresses IL-6 and IL-8 expression in P. gingivalis LPS-stimulated human gingival fibroblasts

Abstract

This study aims to investigate the effect of alpha-mangostin on interleukin (IL)-6 and IL-8 expression in human gingival fibroblasts (HGFs). HGFs were challenged with Porphyromonas gingivalis LPS and then treated with various concentrations of alpha-mangostin. The cytotoxicity was determined using MTS assay and cytokine expressions were evaluated by Real-time PCR and ELISA. The results showed that 5 μg/ml P. gingivalis LPS and alpha-mangostin at 1 µg/ml or less did not affect the viability of HGFs. Alpha-mangostin reduced IL-6 and IL-8 mRNA and protein in P. gingivalis LPS-stimulated HGFs. These findings suggested that alpha-mangostin might be used as an adjunct to the periodontal therapy.

Tormentic acid inhibits LPS-induced inflammatory response in human gingival fibroblasts via inhibition of TLR4-mediated NF-κB and MAPK signalling pathway

“…5 Stimulation of gingival fibroblasts by LPS induces the activation of nuclear factor kappa B (NF-kB) and mitogen-activated protein kinase (MAPK) pathways, which finally results in the release of cytokines, such as tumour necrosis factor alpha (TNF-a), interleukin (IL)-6, and IL-8. 6,7 These inflammatory mediators induce the destruction of connective tissue, and they play important roles in the development of periodontitis.…”

Alpha-mangostin attenuates brain inflammation induced by peripheral lipopolysaccharide administration in C57BL/6J mice

“…To the best of our knowledge, this is the first study demonstrating in vivo effect of α-MG on IL-6 production, although a previous in vitro study showed that α-MG suppresses IL-6 and IL-8 expression in LPS-stimulated human gingival fibroblasts (Yiemwattana and Kaomongkolgit, 2015). IL-6, a pro-inflammatory cytokine which is released after peripheral immune challenge, has been shown to bind to its receptor on brain endothelial cells and to increase COX-2 expression and prostaglandin synthesis (Eskilsson et al, 2014).…”

The Pivotal Neuroinflammatory, Therapeutic and Neuroprotective Role of Alpha-Mangostin

“…An in vitro study [107] revealed that α-MG suppresses IL-6 and IL-8 secretion in LPS-triggered human gingival fibroblasts. Nevertheless, studies [101,108] have proven that IL-6, which was secreted after peripheral immune experimentation, binds to its receptor on brain endothelial cells resulting in upsurge of COX-2 secretion as well as prostaglandin synthesis.…”