Alpha-particle-induced neoplastic transformation in synchronized hybrid cells of HeLa and human skin fibroblasts

Bettega, D.; Calzolari, P.; Piazzolla, A.; Tallone, L.; Redpath, J. L.

doi:10.1080/095530097143022

Cited by 18 publications

(3 citation statements)

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“…At the intermediate dose of 0.27 cGy, the suppression was not statistically significant. In these data [29] the spontaneous transformation frequency of the system was 8.1 ± 0.6 cells per 10 5 survivors, considerably higher than the value measured in Birmingham [12] of 2.8 ± 0.3 cells per 10 5 survivors and of other workers [12,14,15,[33][34][35][36][37], as shown in table 1, which was published by the authors in 2006 [38]. This difference in spontaneous transformation frequency was highlighted by ourselves in a recent letter [38].…”

Section: Publicationmentioning

confidence: 55%

“…Spontaneous transformation frequency × 10 5 (asynchronous cells) ± SE Bettega et al 1997 (Milan) [35] 1 .0 ± 0.3 Mendonca et al (2000) (Indianapolis) [37] 1 .1 ± 0.6 Lewis et al (2001) (Indianapolis and London) [36] 0.6 ± 0.3 Redpath et al (2001) (California) [33] 2 .7 ± 0.3 Frankenberg et al (2002) (Göttingen) [14] 1 .8 ± 0.4 Redpath et al (2003) (California) [34] 2 .9 ± 0.3 Gögglelmann et al 2003 (Neuherberg) [15] 1 .6 ± 0.1 Heyes and Mill (2004) (Birmingham) [12] 2 .8 ± 0.4 Mean of above:…”

Section: Publicationmentioning

confidence: 99%

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Mammography—oncogenecity at low doses

Heyes¹,

Mill²,

Charles³

2009

J. Radiol. Prot.

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Controversy exists regarding the biological effectiveness of low energy x-rays used for mammography breast screening. Recent radiobiology studies have provided compelling evidence that these low energy x-rays may be 4.42 +/- 2.02 times more effective in causing mutational damage than higher energy x-rays. These data include a study involving in vitro irradiation of a human cell line using a mammography x-ray source and a high energy source which matches the spectrum of radiation observed in survivors from the Hiroshima atomic bomb. Current radiation risk estimates rely heavily on data from the atomic bomb survivors, and a direct comparison between the diagnostic energies used in the UK breast screening programme and those used for risk estimates can now be made. Evidence highlighting the increase in relative biological effectiveness (RBE) of mammography x-rays to a range of x-ray energies implies that the risks of radiation-induced breast cancers for mammography x-rays are potentially underestimated by a factor of four. A pooled analysis of three measurements gives a maximal RBE (for malignant transformation of human cells in vitro) of 4.02 +/- 0.72 for 29 kVp (peak accelerating voltage) x-rays compared to high energy electrons and higher energy x-rays. For the majority of women in the UK NHS breast screening programme, it is shown that the benefit safely exceeds the risk of possible cancer induction even when this higher biological effectiveness factor is applied. The risk/benefit analysis, however, implies the need for caution for women screened under the age of 50, and particularly for those with a family history (and therefore a likely genetic susceptibility) of breast cancer. In vitro radiobiological data are generally acquired at high doses, and there are different extrapolation mechanisms to the low doses seen clinically. Recent low dose in vitro data have indicated a potential suppressive effect at very low dose rates and doses. Whilst mammography is a low dose exposure, it is not a low dose rate examination, and protraction of dose should not be confused with fractionation. Although there is potential for a suppressive effect at low doses, recent epidemiological data, and several international radiation risk assessments, continue to promote the linear no-threshold (LNT) model. Finally, recent studies have shown that magnetic resonance imaging (MRI) is more sensitive than mammography in detecting invasive breast cancer in women with a genetic sensitivity. Since an increase in the risk associated with mammographic screening would blur the justification of exposure for this high risk subgroup, the use of other (non-ionising) screening modalities is preferable.

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Section: Publicationmentioning

confidence: 55%

Section: Publicationmentioning

confidence: 99%

Mammography—oncogenecity at low doses

Heyes¹,

Mill²,

Charles³

2009

J. Radiol. Prot.

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“…However, were we to have carried out these experiments more recently, we would have chosen a different model-the CGL transformation assay (Redpath et al 1987). The two systems (CGL and C3H 10T 1 2 ) have recently been shown to have similar responses when used in parallel experiments (Bettega et al 1997).…”

Section: Authors' Replymentioning

confidence: 99%

Transformation of C3H 10T-1/2 cells by low doses of ionising radiation

1999

J. Radiol. Prot.

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All the Letters to the Editor in this issue appear in the same postscript or PDF file. Contents Transformation of C3H 10T-1/2 cells by low doses of ionising radiation B R Scott Lovelace Respiratory Research Institute, PO Box 5890, Albuquerque, NM 87185, USA Reply from: A J Milla, D Bettegab, D Frankenbergc, L Hieberd, M M Lehanee, C J Robertsf and A Sarang a Faculty of Applied Sciences, Frenchay Campus, University of the West of England, Coldharbour Lane, Bristol BS16 1QY, UK b Dipartimento di Fisica, Universitá degli Studi di Milano, Via Celoria, 16, I-20133 Milano, Italy c Klinische Strahlenbiologie und Klinische Strahlenphysik, Georg-August-Universität Göttingen, von Siebold-Straße 3, 3400 Göttingen, Germany d Institut für Strahlenbiologie, GSF - Forschungzentrum, Ingolstadter Landstraße 1, 85764 Oberschleissheim, München, Germany e CRC Department of Medical Oncology, Paterson Institute of Cancer Research, Wilmslow Road, Withington, Manchester M20 4BX, UK f Biotechnology Services, AEA Technology plc, 353 Harwell Laboratory, Didcot, Oxfordshire OX11 0RA, UK g Ente per le Nuove Tecnologie, l'Energia e l'Ambiente, CRE-Casaccia, Settore AMB-BIO, C.P. 2400, 00100 Roma, Italy

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Cell (A549)–Particle (Jasada Bhasma) interactions using Raman spectroscopy

et al. 2008

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Current methods for the evaluation of cell interactions with particles are nonspecific, slow, and invasive to the cells. Raman spectroscopy is a noninvasive technique, and is used in the present study to investigate particle-cell interactions. The main focus of the present study is to employ Raman spectroscopy for investigating the interaction of human lung adenocarcinoma cell line (A549) with the particulate system Jasada Bhasma, a traditional Indian medicine. Jasada Bhasma is a unique preparation of zinc and is traditionally used for the treatment of various diseases like diabetes, age-related eye diseases, and as a health promotional tonic. The Raman spectral analysis is executed by identifying the difference in intracellular DNA/RNA, and proteins and lipids concentration between particles--treated and untreated cells. Comparison between Bhasma-treated and -untreated cells indicates that vibrational peaks corresponding to the DNA/RNA molecule show a significant increase in cells treated with the Jasada Bhasma. Apart from the DNA molecule, several other vibrational peaks related to the protein molecules also show a significant increase in A549 cells after treatment with Bhasma. These results indicate that Bhasma treatment of A549 possibly delays DNA degradation and enables retention of higher amount of protein molecules in the cells.

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Alpha-particle-induced neoplastic transformation in synchronized hybrid cells of HeLa and human skin fibroblasts

Cited by 18 publications

References 18 publications

Mammography—oncogenecity at low doses

Mammography—oncogenecity at low doses

Transformation of C3H 10T-1/2 cells by low doses of ionising radiation

Cell (A549)–Particle (Jasada Bhasma) interactions using Raman spectroscopy

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