Alterações fisiológicas e bioquímicas durante a embebição de sementes de sucupira-preta (Bowdichia virgilioides Kunth.)

Abstract

RESUMO -Nessa pesquisa foi avaliado o comportamento das sementes de sucupira-preta durante a embebição, bem como as modificações em algumas proteínas durante esse processo. As sementes foram embebidas em rolo de papel umedecido, a 30°C e pesadas a cada 6 horas até a protrusão radicular de 50% das sementes, após o que as sementes foram submetidas a análises protéicas pela técnica da eletroforese. A curva de embebição dessa espécie segue um modelo trifásico. Na avaliação dos sistemas enzimáticos, foi observado um comportamento constante para as enzimas superóxido dismutase e malato desidrogenase. Para a enzima álcool desidrogenase, catalase e α-amilase, a intensidade das bandas variou em função do lote utilizado. Para as proteínas resistentes ao calor, foi observada a presença de bandas durante toda a germinação, com alterações na intensidade no decorrer do processo.Termos para indexação: espécie florestal, embebição, isoenzima, LEA proteína, eletroforese. PHYSIOLOGICAL AND BIOCHEMICAL ALTERATIONS DURING GERMINATION OFSUCUPIRA-PRETA (Bowdichia virgilioides Kunth.) SEEDS.ABSTRACT -This research aimed to evaluate the alterations in black sucupira seeds during imbebetion and the modifications in some enzymes and proteins during this process. The seeds were imbibed in wet paper roll at 30°C and weighted for 6 hours until the root protrusion of 50% of the seeds, then the seeds were submitted to the protein analysis by electrophoresis technique. The imbebition curve of the black sucupira has a triphasic model. The evaluation of enzymatic systems shoed the same pattern for the enzymes superoxide dismutase and malate dehidrogenase. For the enzymes alcohol dehidrogenase, catalase and α-amylase, the band intensity varied according to the seed lot used. For the protein resistant to heat, the presence of bands was observed throughout the germination, with alterations in intensity during the process.

Heat-resistant protein expression during germination of maize seeds under water stress

“…Albuquerque et al (2009) also found a reduction in band intensity of heat-resistant protein in seeds soaked for a longer time, near the root protrusion.…”

Substrates and temperatures in the germination of Eriotheca gracilipes seeds

“…Through these studies, it may be inferred that the substrate and the temperature influence the seed germination, because when the germination process is started, metabolic reactions involving the activation of enzymes, hydrolysis, assimilation and mobilization of reserves, stretching and cell division are observed in the seeds (ALBUQUERQUE et al, 2009). Thus, in addition to the physiological activities that vary depending on the initial quality of the seed (MBOFUNG et al, 2013), the structure of the substrate, water retention capacity, aeration establishment and temperature also contribute to the increased percentage of germination (MARTINS et al, 2013).…”

Temperature and pre-germinative treatments for overcoming Acacia farnesiana (L.) Willd. (Fabaceae) seeds dormancy

“…sulfuric acid 10', imb), at 30 O C (Table 2). Chemical and mechanic scarification caused fissures on the seeds testa of this study, which allows the absorption of water and oxygen (Martin et al, 2008), enzymatic activation and energetic reserves hydrolysis, resulting in cell division and elongation and a faster and more constant germination (Albuquerque et al, 2009).…”