Alteration in gut microbiota caused by time‐restricted feeding alleviate hepatic ischaemia reperfusion injury in mice

Abstract

Time‐restricted feeding ( TRF ), that is, no caloric intake for 14‐16 hours each day leads to favourable nutritional outcomes. This study is the first to investigate TRF through a surgical perspective verifying its efficacy against liver ischaemia reperfusion (I/R) injury. We randomly assigned 100 10‐week‐old wild‐type male C57 BL /6 mice into two feeding regimens: TRF and ad libitum access to food. Main outcomes were evaluated at 6, 12 and 24 hours post‐I/R surgery after 12 weeks of intervention. TRF group demonstrated minor liver injury via histological study; lower serum levels of liver enzymes, glucose and lipids; higher concentrations of free fatty acid and β‐hydroxybutyrate; decreased oxidative stress and inflammatory biomarkers; as well as less severe cell apoptosis and proliferation. Further exploration indicated better gut microenvironment and intestinal epithelial tight junction function. TRF employed its positive influence on a wide spectrum of biochemical pathways and ultimately revealed protective effect against hepatic I/R injury possibly through adjusting the gut microbiota. The results referred to a strong indication of adopting better feeding pattern for surgical patients.

Current Knowledge about the Effect of Nutritional Status, Supplemented Nutrition Diet, and Gut Microbiota on Hepatic Ischemia-Reperfusion and Regeneration in Liver Surgery

“…As time-restricted feeding (TRF) is a promising intervention against the worldwide trend of obesity and other metabolic diseases [175], a study conducted in mice investigated whether alteration in gut microbiota caused by TRF could alleviate hepatic I/R injury [164]. The results confirmed the adverse effect of I/R on the gut microbial population.…”

“…The results confirmed the adverse effect of I/R on the gut microbial population. However, TRF prior to surgery reduced the damage, oxidative stress, and inflammatory biomarkers associated with I/R, likely due to intestinal increases in Firmicutes phylum, Clostridia and Bacilli classes, Clostridiales and Lactobacillales orders, and Lachnospiraceae and Ruminococcaceae families, which could be hallmarks of a healthy gut [164].…”

Beneficial Effects of Time-Restricted Eating on Metabolic Diseases: A Systemic Review and Meta-Analysis

“…Aging is associated with a gradual loss of fat-free mass and a gain of excess body fat. We refer to this condition involving high fat mass with low fat-free mass as sarcopenic obesity [57]. TRE reduces this ratio; thus, TRE may be a good therapeutic strategy to treat obesity in elderly patients.…”

Time-restricted feeding reduces the detrimental effects of a high-fat diet, possibly by modulating the circadian rhythm of hepatic lipid metabolism and gut microbiota

“…Studies in as early as 1980s proposed that reducing food availability protected against aging and improved life span in mice [3,4]. Recent researches as well as our studies in rodent animals concerning TRF are increasingly revealing its various bene cial effects, including preventing diet-induced obesity and its associated metabolic disorders [1,[5][6][7][8], preventing in ammatory bowel diseases and colorectal cancer and neurodegenerative disorders [9,10], and alleviating hepatic ischemia reperfusion injury [11]. The rhythm of feeding can greatly in uence the development of liver steatosis and remodel the hepatic circadian metabolome, which suggests a potential mechanism of TRF [12,13].…”