Alteration in Lipid and Protein Profiles of Ovarian Cancer: Similarity to Breast Cancer

Kang, Sokbom; Lee, Aera; Park, Young Seung; Lee, Seok Cheol; Park, Soo‐Jin; Han, Sang Yun; Kim, Kwang Pyo; Kim, Young Hwan; Yoo, Chong Woo; Kim, Hark Kyun

doi:10.1097/igc.0b013e318226c5f5

Cited by 25 publications

(28 citation statements)

References 20 publications

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“…A binary matrix solution was prepared by dissolving 3.5 mg each of DHB and CHCA into 1 ml of 70% methanol containing 0.1% TFA for the positive mode lipid MALDI-MS analysis [29], [30], [40], [41]. 9-aminoacridine (5 mg/ml; dissolved in 6∶4 isopropanol: acetonitrile, v/v) was used for the negative mode analysis [40], [42].…”

Section: Methodsmentioning

confidence: 99%

Analysis of the Phospholipid Profile of Metaphase II Mouse Oocytes Undergoing Vitrification

et al. 2014

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Oocyte freezing confers thermal and chemical stress upon the oolemma and various other intracellular structures due to the formation of ice crystals. The lipid profiles of oocytes and embryos are closely associated with both, the degrees of their membrane fluidity, as well as the degree of chilling and freezing injuries that may occur during cryopreservation. In spite of the importance of lipids in the process of cryopreservation, the phospholipid status in oocytes and embryos before and after freezing has not been investigated. In this study, we employed mass spectrometric analysis to examine if vitrification has an effect on the phospholipid profiles of mouse oocytes. Freshly prepared metaphase II mouse oocytes were vitrified using copper grids and stored in liquid nitrogen for 2 weeks. Fresh and vitrified-warmed oocytes were subjected to phospholipid extraction procedure. Mass spectrometric analyses revealed that multiple species of phospholipids are reduced in vitrified-warmed oocytes. LIFT analyses identified 31 underexpressed and 5 overexpressed phospholipids in vitrified mouse oocytes. The intensities of phosphatidylinositol (PI) {18∶2/16∶0} [M−H]− and phosphatidylglycerol (PG) {14∶0/18∶2} [M−H]− were decreased the most with fold changes of 30.5 and 19.1 in negative ion mode, respectively. Several sphingomyelins (SM) including SM {d38∶3} [M+H]+ and SM {d34∶0} [M+K]+ were decreased significantly in positive ion mode. Overall, the declining trend of multiple phospholipids demonstrates that vitrification has a marked effect on phospholipid profiles of oocytes. These results show that the identified phospholipids can be used as potential biomarkers of oocyte undergoing vitrification and will allow for the development of strategies to preserve phospholipids during oocyte cryopreservation.

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Section: Methodsmentioning

confidence: 99%

Analysis of the Phospholipid Profile of Metaphase II Mouse Oocytes Undergoing Vitrification

et al. 2014

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“…Levels of PC 32:3, 34:1 and 36:2 were higher in ovarian cancers. The authors concluded that modifications in lipid profiles could differentiate ovarian cancer from adjacent normal tissue [80]. The prognostic value of lipidome changes in ovarian neoplasm was investigated by several studies.…”

Section: Lipidomics In Cancer Researchmentioning

confidence: 99%

Advances in Lipidomics for Cancer Biomarkers Discovery

Perrotti

Rosa

Cicalini

et al. 2016

IJMS

164

119

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Lipids play critical functions in cellular survival, proliferation, interaction and death, since they are involved in chemical-energy storage, cellular signaling, cell membranes, and cell–cell interactions. These cellular processes are strongly related to carcinogenesis pathways, particularly to transformation, progression, and metastasis, suggesting the bioactive lipids are mediators of a number of oncogenic processes. The current review gives a synopsis of a lipidomic approach in tumor characterization; we provide an overview on potential lipid biomarkers in the oncology field and on the principal lipidomic methodologies applied. The novel lipidomic biomarkers are reviewed in an effort to underline their role in diagnosis, in prognostic characterization and in prediction of therapeutic outcomes. A lipidomic investigation through mass spectrometry highlights new insights on molecular mechanisms underlying cancer disease. This new understanding will promote clinical applications in drug discovery and personalized therapy.

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“…Recently, several binary matrices have been developed to ionize PLs effectively, including one that we developed ( 17,18 ). With the introduction of a binary matrix, this has become the popular approach to the profi ling and visualization of PLs using MALDI and there have been various applications of binary matrices for IMS of PLs in pathological tissues (19)(20)(21)(22)(23). In this study, we profi led and acquired images of the PL changes associated with AD development using AD model mice and age-matched controls.…”

Section: Ims Analysismentioning

confidence: 99%

Global changes of phospholipids identified by MALDI imaging mass spectrometry in a mouse model of Alzheimer's disease

Hong

Kang

Kim

et al. 2016

Journal of Lipid Research

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This article is available online at http://www.jlr.org Several hypotheses regarding the cause of AD have been proposed, among which the amyloid hypothesis is the most widely accepted ( 2 ). According to this hypothesis, AD is caused by abnormal accumulation of misfolded ␤ -amyloid (A ␤ ), a sequential cleavage product produced from amyloid precursor protein (APP) by ␤ -and ␥ -secretases, and hyperphosphorylated tau protein. Several lines of evidence support the idea that A ␤ can trigger the hyperphosphorylation of tau, resulting in neuronal degeneration in the brain ( 3, 4 ).There is evidence that FFAs are associated with several signaling processes related to the pathogenesis of AD. In particular, palmitic and stearic acid induce AD by triggering hyperphosphorylation of tau protein ( 5 ). Membrane phospholipids (PLs) are a prominent source of FFAs. Under disease conditions, phospholipase enzymes are produced in excess, inducing the hydrolysis of PLs and the release of FFAs. It is reasonable to hypothesize that alterations in PL concentrations are closely related to the pathogenesis of AD, as PLs perform a number of important cellular roles, including stabilization of membrane ion channels, neurotransmission, and the localization of A ␤ plaques to PL cores ( 6, 7 ).As a result of progress in MS, and MALDI-imaging MS (IMS) in particular, the analysis of PL changes during disease progression has become feasible. MALDI-IMS is a unique tool that integrates molecular and histological information together with information attained using Alzheimer's disease (AD), the most common form of dementia, is primarily caused by abnormal protein kinesis and the accumulation of aggregated proteins in the brain. This work was supported

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Alteration in Lipid and Protein Profiles of Ovarian Cancer: Similarity to Breast Cancer

Cited by 25 publications

References 20 publications

Analysis of the Phospholipid Profile of Metaphase II Mouse Oocytes Undergoing Vitrification

Analysis of the Phospholipid Profile of Metaphase II Mouse Oocytes Undergoing Vitrification

Advances in Lipidomics for Cancer Biomarkers Discovery

Global changes of phospholipids identified by MALDI imaging mass spectrometry in a mouse model of Alzheimer's disease

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