Alteration of Citrate Metabolism in Cluster Roots of White Lupin

Kihara, Tomonori; Wada, Tatsumi; Suzuki, Yuji; Hara, Tetsuo; Koyama, Hiroyuki

doi:10.1093/pcp/pcg115

Cited by 58 publications

(41 citation statements)

References 43 publications

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“…pdp41649 for PnSTOP1 and pds27803 for PpSTOP1, developed by the National Bio-Resource Project of the Ministry of Education, Culture, Sports, Science, and Technology, Japan). Putative orthologs of AtSTOP1 in tobacco (cv Xanthi), designated as NtSTOP1, and tea (Camellia sinensis 'Yabukita'), designated as CsSTOP1, and homologs of the Al-inducible citrate-transporting MATE (NtMATE) and ALS3 (NtALS3) in tobacco were isolated by degenerate PCR (for primers, see Supplemental Table S1) followed by 39 and 59 RACE procedures as described previously (Kihara et al, 2003). Total RNA isolated from root samples (see below) was used as the template for RT-PCR and degenerate PCR.…”

Section: Plant Materialsmentioning

confidence: 99%

Characterization of AtSTOP1 Orthologous Genes in Tobacco and Other Plant Species

et al. 2013

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Aluminum (Al) and proton (H + ) tolerances are essential traits for plants to adapt to acid soil environments. In Arabidopsis (Arabidopsis thaliana), these tolerances are mediated by a zinc-finger transcription factor, SENSITIVE TO PROTON RHIZOTOXICITY1 (AtSTOP1), which regulates the transcription of multiple genes critical for tolerance to both stressors. Here, the functions of orthologous proteins (STOP1-like proteins) in other plant species were characterized by reverse genetics analyses and in planta complementation assays. RNA interference of a gene for NtSTOP1 repressed Al and H + tolerances of tobacco (Nicotiana tabacum) roots. Tobacco roots released citrate in response to Al, concomitant with the up-regulated transcription of an ortholog of an Al tolerance gene encoding a citratetransporting multidrug and toxic compound extrusion protein. The RNA interference repression of NtSTOP1 blocked this process and also repressed the transcription of another orthologous gene for Al tolerance, ALUMINUM SENSITIVE3, which encodes a prokaryotetype transporter. These results demonstrated that NtSTOP1 regulates Al tolerance in tobacco through the transcriptional regulation of these genes. The in planta complementation assays revealed that other plant species, including woody plants, a legume, and a moss (Physcomitrella patens), possess functional STOP1-like proteins that can activate several H + and Al-tolerance genes in Arabidopsis. Knocking out the gene encoding the STOP1-like protein decreased the Al tolerance of P. patens. Together, our results strongly suggest that transcriptional regulation by STOP1-like proteins is evolutionarily conserved among land plants and that it confers the ability to survive in acid soils through the transcriptional regulation of Al-and H + -tolerance genes.

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Section: Plant Materialsmentioning

confidence: 99%

Characterization of AtSTOP1 Orthologous Genes in Tobacco and Other Plant Species

et al. 2013

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“…The quantification process followed the manufacturer's protocol. Also, semiquantification of AtALMT1 transcripts by gel image was carried out according to Kihara et al (2003) with modifications. Briefly, 1-mg aliquot of total RNA was reverse transcribed using oligo(dT) primer in the presence of pAW109 RNA (Applied Biosystems) as an internal control for the RT and PCR efficiency.…”

Section: Rna Isolation and Quantitative Rt-pcrmentioning

confidence: 99%

Characterization of AtALMT1 Expression in Aluminum-Inducible Malate Release and Its Role for Rhizotoxic Stress Tolerance in Arabidopsis

et al. 2007

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Malate transporters play a critical role in aluminum (Al) tolerance responses for some plant species, such as Arabidopsis (Arabidopsis thaliana). Here, we further characterize AtALMT1, an Arabidopsis aluminum-activated malate transporter, to clarify its specific role in malate release and Al stress responses. Malate excretion from the roots of accession Columbia was sharply induced by Al, which is concomitant with the induction of AtALMT1 gene expression. The malate release was specific for Al among rhizotoxic stressors, namely cadmium, copper, erbium, lanthanum, sodium, and low pH, which accounts for the specific sensitivity of a null mutant to Al stress. Al-specific malate excretion can be explained by a combined regulation of AtALMT1 expression and activation of AtALMT1 protein, which is specific for Al. Although low pH treatment slightly induced gene expression, other treatments did not. In addition, malate excretion in Al-activated seedlings was rapidly stopped by removing Al from the solution. Other rhizotoxic stressors were not effective in maintaining malate release. Protein kinase and phosphatase inhibitor studies indicated that reversible phosphorylation was important for the transcriptional and posttranslational regulation of AtALMT1. AtALMT1 promoter-β-glucuronidase fusion lines revealed that AtALMT1 has restricted expression within the root, such that unnecessary carbon loss is likely minimized. Lastly, a natural nonsense mutation allele of AtALMT1 was identified from the Al-hypersensitive natural accession Warschau-1.

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“…In juvenile cluster roots, most of the genes involved in the TCA cycle show increased transcript levels, whereas they show lower abundance in mature cluster roots [30]. This is most striking for pyruvate dehydrogenase [34] and isocitrate dehydrogenase [59], whose increased transcript abundance favours the production of citrate. Although similar approaches have been taken in the work of Secco et al [33] and Wang et al [34], a direct comparison cannot be made in all points, since the developmental stages differed.…”

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Plant adaptations to severely phosphorus-impoverished soils

Lambers

Martinoia

Renton

2015

Current Opinion in Plant Biology

173

121

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Mycorrhizas play a pivotal role in phosphorus (P) acquisition of plant roots, by enhancing the soil volume that can be explored. Non-mycorrhizal plant species typically occur either in relatively fertile soil or on soil with a very low P availability, where there is insufficient P in the soil solution for mycorrhizal hyphae to be effective. Soils with a very low P availability are either old and severely weathered or relatively young with high concentrations of oxides and hydroxides of aluminium and iron that sorb P. In such soils, cluster roots and other specialised roots that release P-mobilising carboxylates are more effective than mycorrhizas. Cluster roots are ephemeral structures that release carboxylates in an exudative burst. The carboxylates mobilise sparingly-available sources of soil P. The relative investment of biomass in cluster roots and the amount of carboxylates that are released during the exudative burst differ between species on severely weathered soils with a low total P concentration and species on young soils with high total P concentrations but low P availability. Taking a modelling approach, we explore how the optimal cluster-root strategy depends on soil characteristics, thus offering insights for plant breeders interested in developing crop plants with optimal cluster-root strategies. DOI: https://doi.org/10.1016/j.pbi. 2015.04.002 Posted at the Zurich Open Repository and Archive, University of Zurich ZORA URL: https://doi.org/10.5167/uzh-121418 Accepted Version Originally published at: Lambers, Hans; Martinoia, Enrico; Renton, Michael (2015). Plant adaptations to severely phosphorusimpoverished soils. Current Opinion in Plant Biology,[25][26][27][28][29][30][31] AbstractMycorrhizas play a pivotal role in phosphorus (P) acquisition of plant roots, by enhancing the soil volume that can be explored. Non-mycorrhizal plant species typically occur either in relatively fertile soil or on soil with a very low P availability, where there is insufficient P in the soil solution for mycorrhizal hyphae to be effective. Soils with a very low P availability are either old and severely weathered or relatively young with high concentrations of oxides and hydroxides of aluminium and iron that sorb P. In such soils, cluster roots and other specialised roots that release P-mobilising carboxylates are more effective than mycorrhizas. Cluster roots are ephemeral structures that release carboxylates in an exudative burst. The carboxylates mobilise sparingly-available sources of soil P. The relative investment of biomass in cluster roots and the amount of carboxylates that are released during the exudative burst differ between species on severely weathered soils with a low total P concentration and species on young soils with high total P concentrations but low P availability. Taking a modelling approach, we explore how the optimal cluster-root strategy depends on soil characteristics, thus offering insights for plant breeders interested in developing crop plants with optimal cluster-root strat...

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Alteration of Citrate Metabolism in Cluster Roots of White Lupin

Cited by 58 publications

References 43 publications

Characterization of AtSTOP1 Orthologous Genes in Tobacco and Other Plant Species

Characterization of AtSTOP1 Orthologous Genes in Tobacco and Other Plant Species

Characterization of AtALMT1 Expression in Aluminum-Inducible Malate Release and Its Role for Rhizotoxic Stress Tolerance in Arabidopsis

Plant adaptations to severely phosphorus-impoverished soils

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