Alteration of in vitro bone metabolism and tooth formation by zinc

Togari, Akifumi; Seiichi, Arakawa; Arai, M.; Matsumoto, Shosei

doi:10.1016/0306-3623(93)90360-a

Cited by 29 publications

(23 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4). Although MVs isolated from control cultures contained little annexin II, V, and VI, vesicles isolated from RA-treated cultures contained significantly higher amounts 45 Ca 2ϩ uptake by MVs isolated from untreated, RA-treated, or RA/Zn 2ϩ -treated hypertrophic chondrocyte cultures and the effect of CHAPS treatment on 45 Ca 2ϩ uptake by these fractions. MVs were isolated from untreated chondrocyte cultures or cultures treated with RA (35 nM) or RA (35 nM) and ZnCl 2 (added 100 µM) for 6 days.…”

Section: Resultsmentioning

confidence: 98%

“…MVs were isolated from untreated chondrocyte cultures or cultures treated with RA (35 nM) or RA (35 nM) and ZnCl 2 (added 100 µM) for 6 days. The pellets of each MV fraction (100 µg of protein) were either resuspended in 1 ml 45 Ca 2ϩ containing SCL and used for 45 Ca 2ϩ uptake studies or were first treated with 1% CHAPS (ϩCHAPS) and then used for 45 Ca 2ϩ uptake studies as described in Materials and Methods. Triplicate samples were used for uptake studies.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, Zn 2ϩ treatment of mouse calvaria organ cultures led to decreased mineralization, whereas extensive cartilage mineralization was observed in Zn 2ϩ -deficient rats. (20,45) All of these findings indicate that Zn 2ϩ plays a major role as an inhibitory factor in controlling mineralization of skeletal tissues.…”

Section: Figmentioning

confidence: 98%

“…After centrifugation, the pellets were resuspended in SCL and 45 Ca 2ϩ uptake was measured. 45 Ca 2ϩ uptake studies: MV mineralization was assayed by 45 Ca 2ϩ accumulation as described previously. (11) Briefly, MVs (100 µg protein) were incubated in 1 ml of 45 Ca 2ϩ containing SCL at 37°C.…”

Section: Treatment and Ca 2ϩ Uptake Studies Of Mvsmentioning

confidence: 99%

“…45 Ca 2ϩ uptake studies: MV mineralization was assayed by 45 Ca 2ϩ accumulation as described previously. (11) Briefly, MVs (100 µg protein) were incubated in 1 ml of 45 Ca 2ϩ containing SCL at 37°C. After 24-h incubation, 100-µl aliquots of the vesicle suspensions were sampled by microfiltration through Millipore HA filters (0.45 µm pore size) and the filters were washed twice.…”

Section: Treatment and Ca 2ϩ Uptake Studies Of Mvsmentioning

confidence: 99%

See 4 more Smart Citations

Regulatory Roles of Zinc in Matrix Vesicle-Mediated Mineralization of Growth Plate Cartilage

Kirsch

Harrison

Worch

et al. 2000

Journal of Bone and Mineral Research

View full text Add to dashboard Cite

Zinc (Zn 2؉ ) has long been known to play important roles in mineralization and ossification of skeletal tissues, but the mechanisms of Zn 2؉ action are not well understood. In this study we investigated the effects of Zn 2؉ on mineralization in a cell culture system in which terminal differentiation and mineralization of hypertrophic growth plate chondrocytes was induced by retinoic acid (RA) treatment. Addition of Zn 2؉ to RA-treated cultures decreased mineralization in a dose-dependent manner without affecting alkaline phosphatase (APase) activity. Characterization of matrix vesicles (MVs), particles that initiate the mineralization process, revealed that vesicles isolated from RA-treated and RA/Zn 2؉ -treated cultures showed similar APase activity, but vesicles from RA/Zn 2؉ -treated cultures contained significantly less Ca 2؉ and P i . MVs isolated from RA-treated cultures were able to take up Ca 2؉ and mineralize in vitro, whereas vesicles isolated from RA/Zn 2؉ -treated cultures were not able to do so. Detergent treatment, which ruptures the MV membrane and exposes preformed intravesicular Ca 2؉ -P i -phospholipid complexes, did not restore the Ca 2؉

show abstract

Section: Resultsmentioning

confidence: 98%