Alteration of major vault protein in human glioblastoma and its relation with EGFR and PTEN status

Navarro, Lara; Gil-Benso, Rosario; Megías, Javier; Muñoz-Hidalgo, Lisandra; San-Miguel, Teresa; Callaghan, Robert C.; González-Darder, José M.; López‐Ginés, Concha; Cerdá-Nicolás, Miguel

doi:10.1016/j.neuroscience.2015.04.005

Cited by 19 publications

(14 citation statements)

References 31 publications

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“…Overexpression of MVP/LRP is observed in many types of malignant neoplasms, includ ing brain tumors such as glioblastomas and astrocytomas [10]. In some studies, the clinical significance of MVP/LRP expression for assessing glioblastoma progres sion and as a target for advanced approaches for treating gliomas has been discussed [11,12].…”

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confidence: 99%

Connection between proliferation rate and temozolomide sensitivity of primary glioblastoma cell culture and expression of YB-1 and LRP/MVP

Моисеева

Susova

Митрофанов

et al. 2016

Biochemistry Moscow

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Glioblastomas (GBL) are the most common and aggressive brain tumors. They are distinguished by high resistance to radiation and chemotherapy. To find novel approaches for GBL classification, we obtained 16 primary GBL cell cultures and tested them with real-time PCR for mRNA expression of several genes (YB-1, MGMT, MELK, MVP, MDR1, BCRP) involved in controlling cell proliferation and drug resistance. The primary GBL cultures differed in terms of proliferation rate, wherein a group of GBL cell cultures with low proliferation rate demonstrated higher resistance to temozolomide. We found that GBL primary cell cultures characterized by high proliferation rate and lower resistance to temozolomide expressed higher mRNA level of the YB-1 and MDR1 genes, whereas upregulated expression of MVP/LRP mRNA was a marker in the group of GBL with low proliferation rate and high resistance. A moderate correlation between expression of YB-1 and MELK as well as YB-1 and MDR1 was found. In the case of YB-1 and MGMT expression, no correlation was found. A significant negative correlation was revealed between mRNA expression of MVP/LRP and MELK, MDR1, and BCRP. No correlation in expression of YB-1 and MVP/LRP genes was observed. It seems that mRNA expression of YB-1 and MVP/LRP may serve as a marker for GBL cell cultures belonging to distinct groups, each of which is characterized by a unique pattern of gene activity.

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confidence: 99%

Connection between proliferation rate and temozolomide sensitivity of primary glioblastoma cell culture and expression of YB-1 and LRP/MVP

Моисеева

Susova

Митрофанов

et al. 2016

Biochemistry Moscow

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“…Epidermal growth factor receptor gene amplification and phosphatase and tensin homolog mutations are more common in primary GBM than secondary GBM. In secondary GBM, mutations occur more commonly in the isocitrate dehydrogenase 1 or 2 and TP53 genes (5,6). In ~80% of GBMs, there are also changes in tyrosine kinase activity transmembrane receptor signaling pathways, the p53 pathway (TP53/mouse double minute 2 homolog/p14ARF), the phosphorylated retinoblastoma (RB) pathway [RB1/cyclin-dependant kinase (CDK) inhibitor 2A/CDK4] and the telomerase reverse transcriptase promoter region (pTERT) (7,8).…”

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confidence: 99%

STAT3‑PTTG11 abrogation inhibits proliferation and induces apoptosis in malignant glioma cells

Cui

et al. 2020

Oncol Lett

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Pituitary tumor transforming gene 1 (PTTG11) is abundantly expressed in glioma. Our previous study demonstrated that the downregulation of PTTG11 gene expression significantly inhibited the proliferation, migration and invasion ability, and increased the apoptosis of SHG44 glioma cells. However, the molecular mechanisms that regulate PTTG11 and its actions remain elusive. In the present study, CCK-8 and flow cytometry assays were used to assess the proliferation/viability and apoptosis, respectively, of the human glioma U251 cell line. STAT3-PTTG1 signals were further evaluated by western blotting. The findings of the present study revealed that STAT3 induced PTTG11 expression, which subsequently induced downstream c-Myc and Bcl-2 expression while inhibiting Bax expression, thereby promoting cell viability and inhibiting apoptosis. PTTG11 suppression via siRNA inhibited the viability and increased the apoptosis of glioma cells induced by the STAT3 activator S3I-201. c-Myc and Bcl-2 expression was suppressed by PTTG11 inhibition. The findings of the present study suggest that the STAT3-PTTG11 signaling pathway may play an important role in glioma progression by regulating cell proliferation and apoptosis.

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“…These receptors play a major role in the modulation of diverse cellular functions, such as cell proliferation, differentiation, adhesion, survival, motility [ 3 ] and programmed cell death [ 6 ] that are often dysregulated, generating a cascade of responses able to promote the formation and progression of various malignancies such as: glioblastoma [ 7 ], muscle invasive bladder cancer [ 8 ], non-small-cell lung cancer [ 9 ], squamous cell carcinoma of the skin [ 10 ], gastric cancer [ 11 ] and breast cancer [ 12 ], via aberrant overexpression, kinase activation and mutation. Studies have demonstrated that some of these receptors have special characteristics, such as HER2 and HER3; HER2 possesses no known natural ligands [ 13 , 14 ] and HER3, the only member of the HER family lacking intrinsic tyrosine kinase activity [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Natural Products as Chemopreventive Agents by Potential Inhibition of the Kinase Domain in ErbB Receptors

2017

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Small molecules found in natural products provide therapeutic benefits due to their pharmacological or biological activity, which may increase or decrease the expression of human epidermal growth factor receptor (HER), a promising target in the modification of signaling cascades involved in excessive cellular growth. In this study, in silico molecular protein-ligand docking protocols were performed with AutoDock Vina in order to evaluate the interaction of 800 natural compounds (NPs) from the NatProd Collection (), with four human HER family members: HER1 (PDB: 2ITW), HER2 (PDB: 3PP0), HER3 (PDB: 3LMG) and HER4 (PDB: 2R4B). The best binding affinity values (kcal/mol) for docking pairs were obtained for HER1-podototarin (−10.7), HER2-hecogenin acetate (−11.2), HER3-hesperidin (−11.5) and HER4-theaflavin (−10.7). The reliability of the theoretical calculations was evaluated employing published data on HER inhibition correlated with in silico binding calculations. IC50 values followed a significant linear relationship with the theoretical binding Affinity data for HER1 (R = 0.656, p < 0.0001) and HER2 (R = 0.543, p < 0.0001), but not for HER4 (R = 0.364, p > 0.05). In short, this methodology allowed the identification of several NPs as HER inhibitors, being useful in the discovery and design of more potent and selective anticancer drugs.

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Alteration of major vault protein in human glioblastoma and its relation with EGFR and PTEN status

Cited by 19 publications

References 31 publications

Connection between proliferation rate and temozolomide sensitivity of primary glioblastoma cell culture and expression of YB-1 and LRP/MVP

Connection between proliferation rate and temozolomide sensitivity of primary glioblastoma cell culture and expression of YB-1 and LRP/MVP

STAT3‑PTTG11 abrogation inhibits proliferation and induces apoptosis in malignant glioma cells

Natural Products as Chemopreventive Agents by Potential Inhibition of the Kinase Domain in ErbB Receptors

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