Alteration of oxidative stress parameters in red blood cells of rats after chronic in vivo treatment with cisplatin and selenium

Abstract

In this study we evaluated the possible protective effects of selenium (Se) on hematological and oxidative stress parameters in rats chronically treated with cisplatin (cisPt). Four groups of Wistar albino rats were examined: a control, untreated rats (I), rats treated with Se (II), rats treated with cisPt (III), and rats treated with Se and cisPt (IV). All animals were treated for 5 days successively and killed 24 h after the last treatment. Hematological and oxidative stress parameters were followed in whole blood and red blood cells (RBC). Results showed that the chronic application of Se was followed by a higher number of reticulocytes and platelets, increased lipid peroxidation and GSH content in the RBC. Cisplatin treatment induced depletion of RBC and platelet numbers and an elevation of the superoxide anion, nitrites and glutathione levels. Se and cisPt co-treatment was followed by an elevation of the hematological parameters and the recovery of the glutathione status when compared to the control and cisPt-treated rats

Protective effect of aged garlic extract against the oxidative stress induced by cisplatin on blood cells parameters and hepatic antioxidant enzymes in rats

“…However, the increase in the WBCs number could be the consequence of infection and inflammation during CP treatment and CP metabolism in the experimental rats. While, elevation of HTC value in CP-treated rats could be related to loss of the body fluid and hemoconcentration that resulted from the toxic effect of CP on the function of rat kidney [22] .…”

“…Moreover, Marković et al [22] stated that aside from the reduction in RBC number, chronic application of CP induced a reduction in the number of platelets and an increase in the number WBCs in the blood of rats. The decrease in platelets count might be due to CP inhibiting bone marrow activity or could be due to decreased production or increased consumption of platelets or due to the increased platelets aggregation [23] .…”

Haematological Evaluation of Sprague-Dawley Rats Infected With Pasteurella Multocida and Administered Bdellovibrio Bacteriovorus (Atcc™ 1534) as Therapy

“…The consistently lower haemoglobin concentrations in rats injected with P. multocida and with combinations of B. bacteriovorus and P. multocida than in un-injected controls may be related to the low RBC counts, as haemoglobin in all mammals is transported by the RBCs. Also, according to Marković et al (2011) several substances may affect rat haematologic parameters, however, the exact mechanism which caused lowered haemoglobin levels, observed in this study, remains unclear.…”

Ameliorative Role of Bee Honey and Royal Jelly Against Cisplatin Induced Alteration in Hematological Parameters in Male Wister Albino Rat

“…[30]. Cisplatin reduced the platelet count in rats under experiment [25] and depleted the platelet number and caused cumulative anemia in rats [13].…”

“…This relation could be explained through different mechanisms, including the destruction of bone marrow cells or increased osmotic fragility of RBC. Thus, cisplatin therapy might lead to anemia as a result of either suppression of the activity of hematopoietic tissues, impaired erythropoesis, and accelerated RBCs destruction because of the altered RBCs membrane permeability, increased RBCs mechanical fragility, and/or defective iron metabolism [13].…”