Alteration of Stimulus-Specific Guard Cell Calcium Oscillations and Stomatal Closing in
            <i>Arabidopsis det3</i>
            Mutant

Allen, Grant; Chu, Sarah P.; Schumacher, Karin; Shimazaki, Chad T.; Vafeados, Dionne; Kemper, Andrea; Hawke, Scott D.; Tallman, Gary; Tsien, Roger Y.; Harper, Jeffrey F.; Chory, Joanne; Schroeder, Julian I.

doi:10.1126/science.289.5488.2338

Cited by 456 publications

(376 citation statements)

References 26 publications

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“…In wild-type Arabidopsis guard cells, ABA, oxidative stress, cold, and external calcium elicited Ca 2+ oscillations of differing amplitudes and frequencies and induced stomatal closure. In the det3 mutant guard cells although the cold and ABA-induced Ca 2þ cyt oscillations and resulting stomatal closure were normal, external Ca 2+ and oxidative stress induced a nonoscillating, prolonged Ca 2þ cyt increase and these stimuli no longer induced stomatal closure [136]. This result indirectly implicates the V-ATPase in control of Ca 2+ release, likely via effects on tonoplast membrane potential.…”

Section: Vacuolar Ion Transporters: Properties and Regulationmentioning

confidence: 88%

Roles of ion channels and transporters in guard cell signal transduction

2007

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Stomatal complexes consist of pairs of guard cells and the pore they enclose. Reversible changes in guard cell volume alter the aperture of the pore and provide the major regulatory mechanism for control of gas exchange between the plant and the environment. Stomatal movement is facilitated by the activity of ion channels and ion transporters found in the plasma membrane and vacuolar membrane of guard cells. Progress in recent years has elucidated the molecular identities of many guard cell transport proteins, and described their modulation by various cellular signal transduction components during stomatal opening and closure prompted by environmental and endogenous stimuli.

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Section: Vacuolar Ion Transporters: Properties and Regulationmentioning

confidence: 88%

Roles of ion channels and transporters in guard cell signal transduction

2007

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“…While relatively little is known about the primary receptors that sense these stresses, several downstream signaling cascades have been identified and studied in detail. Stresses may lead to an increase in reactive oxygen species (ROS), cytosolic Ca 2+ or inositol phosphates, which in turn can induce signaling events further downstream (Allen et al, 2000;Xiong et al, 2002;Apel and Hirt, 2004;Gao et al, 2004;Pitzschke et al, 2006). Transmission of these signals includes post-translational modifications such as phosphorylation, ubiquitination and sumoylation of important regulatory factors (Halfter et al, 2000;Dong et al, 2006;Miura et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Stress‐induced changes in the Arabidopsis thaliana transcriptome analyzed using whole‐genome tiling arrays

et al. 2009

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SUMMARYThe responses of plants to abiotic stresses are accompanied by massive changes in transcriptome composition. To provide a comprehensive view of stress-induced changes in the Arabidopsis thaliana transcriptome, we have used whole-genome tiling arrays to analyze the effects of salt, osmotic, cold and heat stress as well as application of the hormone abscisic acid (ABA), an important mediator of stress responses. Among annotated genes in the reference strain Columbia we have found many stress-responsive genes, including several transcription factor genes as well as pseudogenes and transposons that have been missed in previous analyses with standard expression arrays. In addition, we report hundreds of newly identified, stress-induced transcribed regions. These often overlap with known, annotated genes. The results are accessible through the Arabidopsis thaliana Tiling Array Express (At-TAX) homepage, which provides convenient tools for displaying expression values of annotated genes, as well as visualization of unannotated transcribed regions along each chromosome.

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“…Specifically, H 2 O 2 can interact with specific Cys residues, transforming the functional group of the Cys into sulfenic acid, altering formation of disulfide bonds between two separate proteins or between nearby Cys residues of the same protein, in a transient, reversible fashion (Poole et al, 2004;Poole and Nelson, 2008). In guard cells, this ROS burst is believed to target calcium channels to promote the influx of calcium across the plasma membrane and the release of calcium from internal stores (McAinsh et al, 1996;Allen et al, 2000;Cho et al, 2009). Increased cytosolic calcium levels induce activation of anion efflux channels located on the plasma membrane (Hedrich et al, 1990;Schroeder and Hagiwara, 1990;Chen et al, 2010;Wang et al, 2013), triggering stomatal closure.…”

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confidence: 99%

Ethylene-Induced Flavonol Accumulation in Guard Cells Suppresses Reactive Oxygen Species and Moderates Stomatal Aperture

2014

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Guard cell swelling controls the aperture of stomata, pores that facilitate gas exchange and water loss from leaves. The hormone abscisic acid (ABA) has a central role in regulation of stomatal closure through synthesis of second messengers, which include reactive oxygen species (ROS). ROS accumulation must be minimized by antioxidants to keep concentrations from reaching damaging levels within the cell. Flavonols are plant metabolites that have been implicated as antioxidants; however, their antioxidant activity in planta has been debated. Flavonols accumulate in guard cells of Arabidopsis thaliana, but not surrounding pavement cells, as visualized with a flavonolspecific dye. The expression of a reporter driven by the promoter of CHALCONE SYNTHASE, a gene encoding a flavonol biosynthetic enzyme, in guard cells, but not pavement cells, suggests guard cell-specific flavonoid synthesis. Increased levels of ROS were detected using a fluorescent ROS sensor in guard cells of transparent testa4-2, which has a null mutation in CHALCONE SYNTHASE and therefore synthesizes no flavonol antioxidants. Guard cells of transparent testa4-2 show more rapid ABA-induced closure than the wild type, suggesting that flavonols may dampen the ABA-dependent ROS burst that drives stomatal closing. The levels of flavonols are positively regulated in guard cells by ethylene treatment in the wild type, but not in the ethylene-insensitive2-5 mutant. In addition, in both ethyleneoverproducing1 and ethylene-treated wild-type plants, elevated flavonols lead to decreasing ROS and slower ABA-mediated stomatal closure. These results are consistent with flavonols suppressing ROS accumulation and decreasing the rate of ABA-dependent stomatal closure, with ethylene-induced increases in guard cell flavonols modulating these responses.

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Alteration of Stimulus-Specific Guard Cell Calcium Oscillations and Stomatal Closing in Arabidopsis det3 Mutant

Cited by 456 publications

References 26 publications

Roles of ion channels and transporters in guard cell signal transduction

Roles of ion channels and transporters in guard cell signal transduction

Stress‐induced changes in the Arabidopsis thaliana transcriptome analyzed using whole‐genome tiling arrays

Ethylene-Induced Flavonol Accumulation in Guard Cells Suppresses Reactive Oxygen Species and Moderates Stomatal Aperture

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