Alteration of the biochemical properties of female gonads and vitellins in the clam Mya arenaria at contaminated sites in the Saguenay Fjord

Gagné, François; Blaise, C.; Pellerin, J.; Gauthier-Clerc, Sophie

doi:10.1016/s0141-1136(01)00122-2

Cited by 67 publications

(38 citation statements)

References 47 publications

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“…Some proteins in invertebrates have been verified to be affected by EDCs. Vitellin levels have been used as a biomarker of various EDCs in mussel (Gagné et al 2001), clam (Gagné et al 2002) and shrimp (Oberdorster et al 2000). Aromatase activity was reported to be inhibited by tributyltin (Tsai et al 2001), while HSP60 and HSP70 were stimulated by various EDCs (Planelló et al 2008;Sole et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Acute toxicity of nonylphenols and bisphenol A to the embryonic development of the abalone Haliotis diversicolor supertexta

et al. 2011

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Acute toxic effects and mechanisms of two typical endocrine disrupting chemicals, nonylphenols (NPs) and bisphenol A (BPA), to the embryonic development of the abalone Haliotis diversicolor supertexta, were investigated by the two-stage embryo toxicity test. The 12-h median effective concentrations (EC(50)) of NPs and BPA to the trochophore development were 1016.22 and 30.72 μg L(-1), respectively, and the respective 96-h EC(50) values based on the completion of metamorphosis (another experimental endpoint) were reduced to 11.65 and 1.02 μg L(-1). Longer exposure time and magnified exposure concentrations in the benthic diatom, that serves as both food source and settlement substrate during the metamorphosis, via bioaccumulation, led to the higher sensitivity of metamorphosis to target EDCs compared with the trochophore development. The hazard concentrations for 5% of the species (HC(5)) could be employed as the safety thresholds for the embryonic development of the abalone. The 12-h HC(5) values of NPs and BPA were 318.68 and 13.93 μg L(-1), respectively, and the respective 96-h HC(5) values were 0.99 and 0.18 μg L(-1), which were at environmentally relevant levels. Results of proteomic responses revealed that NPs and BPA altered various functional proteins in the abalone larvae with slight differences between each chemical and affected various physiological functions, such as energy and substance metabolism, cell signalling, formation of cytoskeleton and cilium, immune and stress responses at the same time, leading to the failure of metamorphosis.

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Section: Discussionmentioning

confidence: 99%

Acute toxicity of nonylphenols and bisphenol A to the embryonic development of the abalone Haliotis diversicolor supertexta

et al. 2011

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“…Indeed, Gagné et al (2002) demonstrated that both male and female freshwater mussels, Elliptio complanata, deployed 1.5 and 5 km downstream from a sewage treatment plant in the St. Lawrence River, Canada, exhibited increases in alkali-liable phosphates (ALP) (a surrogate vitellogenin estimate) compared to reference locations. Male blue mussels, Mytilus galloprovincialis, deployed in Venice canals (receiving raw sewage) have also been shown to exhibit increased ALP levels in hemolymph compared to reference locations (Pampanin et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Exposure to 17α-ethynylestradiol causes dose and temporally dependent changes in intersex, females and vitellogenin production in the Sydney rock oyster

et al. 2010

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Although mounting evidence suggests exposure to estrogenic contaminants increases vitellogenin production in molluscs, demonstration of dose-response relationships and knowledge of the temporal nature of the vitellogenin response with continual exposure is currently lacking for biomarker utility. To address this knowledge gap, adult Sydney rock oysters, Saccostrea glomerata, were exposed to a range of environmentally relevant concentrations of 17α-ethynylestradiol (EE2) (0, 6.25, 12.5, 25 or 50 ng/l) in seawater under laboratory conditions. Vitellogenin induction and gonadal development was assessed following 4, 21 and 49 days exposure to EE2. Vitellogenin was found to increase in a dose dependent manner with EE2 exposure for females (4 and 49 days) and males (4 and 21 days). Histological examination of gonads revealed a number of individuals exhibited intersex (ovotestis) in 50 ng/l EE2 (after 21 days) and in 6.25 and 12.5 ng/l EE2 (after 49 days). Furthermore, a significant shift towards females was observed following 49 days exposure at 50 ng/l EE2 suggesting estrogenic exposure is capable of facilitating a progression for protandric males from male-intersex-female gametal status. Increases in female vitellogenin (4 days) were predictive of later increases in female developmental stages at 21 days and increases in oocyte area following 49 days. Male vitellogenin (4 days) was predictive of decreased male percentages and lower male developmental stages at 49 days. Vitellogenin in S. glomerata is a predictive biomarker of estrogenic exposure and effect if sampled soon after exposure and at the commencement of a gonadal development cycle.

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“…In estradiol-treated trout, hepatic MT remains at basal levels until the Vg mRNA levels begin to decline and Zn is released from high-molecular-weight proteins (Olsson and Kling, 1995), because vitellogenin is a zinc-containing protein in Xenopus leavis (Montorzi et al 1995) and in Mya arenaria clams (Gagné et al 2002). In the present study, zinc levels in the gonad appeared to be negatively related to vitellogenin-like proteins which are egg yolk proteins whose synthesis are under the influence of estrogens, in part at least (Gagné et al 2001).…”

Section: Discussionmentioning

confidence: 99%

Changes in Metallothionein Levels in Freshwater Mussels Exposed to Urban Wastewaters: Effects from Exposure to Heavy Metals?

Gagné¹,

Gagnon²,

Turcotte³

et al. 2007

Biomark�Insights

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Municipal effl uents are complex mixtures of compounds such as heavy metals, aromatic and aliphatic hydrocarbons, and micro-organisms and are released in aquatic ecosystems. The purpose of this study was to verify whether changes in metallothioneins (MT) were associated with the accumulation of labile metals in tissue of freshwater mussels exposed to the dispersion plume of a major municipal effl uent. Mussels were placed in experimental cages deployed at sites 1.5 km upstream, 8 km downstream and 12 km downstream of the outfall of a major, primary-treated municipal effl uent in the St. Lawrence River (Québec, Canada). Mussels were analysed for MT and labile zinc levels in their gonads, gills and digestive glands. Lipogenic enzyme (isocitrate and glucose-6-phosphate dehydrogenase) and arachidonic acid cyclooxygenase (COX) activities were also measured in gonad and gill tissues. Although MT was induced in all the tissues examined, the results showed that labile zinc levels were signifi cantly reduced in gill and gonad tissues, with an increase observed only at the 12 km downstream site in the digestive gland. COX activity was readily induced in gills and gonads. Glucose-6-phosphate dehydrogenase activity was reduced at both downstream sites, but isocitrate dehydrogenase activity was signifi cantly induced at the farthest (12 km) site. Analysis of covariance revealed that MT levels in gills were more infl uenced by COX activity than with distance in the dispersion plume and was negatively correlated with labile zinc levels. In conclusion, MT induction was inversely related to the levels of labile zinc but positively so with the infl ammation biomarker COX. Hence, the induction of MT in mussels exposed to the municipal effl uent of a large city appears to be associated with either infl ammatory processes or as compensation for the loss of labile essential metals. We propose that the simple and complimentary parameters of labile zinc and COX evaluations be used to link MT induction with divalent heavy metal exposure in environmental studies dealing with various type of contaminants in such complex contaminant mixture effl uents.

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Alteration of the biochemical properties of female gonads and vitellins in the clam Mya arenaria at contaminated sites in the Saguenay Fjord

Cited by 67 publications

References 47 publications

Acute toxicity of nonylphenols and bisphenol A to the embryonic development of the abalone Haliotis diversicolor supertexta

Acute toxicity of nonylphenols and bisphenol A to the embryonic development of the abalone Haliotis diversicolor supertexta

Exposure to 17α-ethynylestradiol causes dose and temporally dependent changes in intersex, females and vitellogenin production in the Sydney rock oyster

Changes in Metallothionein Levels in Freshwater Mussels Exposed to Urban Wastewaters: Effects from Exposure to Heavy Metals?

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