Alteration of vaginal microbiota in patients with recurrent miscarriage

Jiao, Xuejuan; Zhang, Lanling; Du, Daolin; Wang, Lingling; Song, Qisheng; Liu, Shuyu

doi:10.1080/01443615.2021.1904851

Cited by 17 publications

(23 citation statements)

References 29 publications

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“…The characteristics of the microbiota harbored in vaginal secretions in RSA have been revealed in several studies. Studies have demonstrated that two genera ( Atopobium and Prevotella ) exhibited significantly greater abundance, along with higher expression of CCL2/CCL8/CCL3/CCL4/CCL5, in RSA patients ( 10 – 13 ). However, the abundance of Gardnerella in RSA patients remains controversial ( 10 , 13 ).…”

Section: Introductionmentioning

confidence: 99%

An Altered Microbiota in the Lower and Upper Female Reproductive Tract of Women with Recurrent Spontaneous Abortion

Liu

Yang

et al. 2022

Microbiol Spectr

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Section: Introductionmentioning

confidence: 99%

An Altered Microbiota in the Lower and Upper Female Reproductive Tract of Women with Recurrent Spontaneous Abortion

Liu

Yang

et al. 2022

Microbiol Spectr

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“…In addition, F. nucleatum has been previously associated with preterm labor, since it was found in greater abundance in preterm placental membranes than at term ( Doyle et al., 2014 ). To the best of our knowledge, this is the first time that Fusobacterium got linked to the risk of first trimester miscarriage, considering that previous investigations highlighted the potential role of other microorganisms, such as Finegoldia, Coprococcus, Roseburia, Atopobium , and Prevotella ( Al-Memar et al., 2020 ; Xu et al., 2020 ; Jiao et al., 2021 ; Liu et al., 2021 ).…”

Section: Discussionmentioning

confidence: 79%

A Deep Look at the Vaginal Environment During Pregnancy and Puerperium

Morselli

Camboni

Ceccarani

et al. 2022

Front. Cell. Infect. Microbiol.

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A deep comprehension of the vaginal ecosystem may hold promise for unraveling the pathophysiology of pregnancy and may provide novel biomarkers to identify subjects at risk of maternal-fetal complications. In this prospective study, we assessed the characteristics of the vaginal environment in a cohort of pregnant women throughout their different gestational ages and puerperium. Both the vaginal bacterial composition and the vaginal metabolic profiles were analyzed. A total of 63 Caucasian women with a successful pregnancy and 9 subjects who had a first trimester miscarriage were enrolled. For the study, obstetric examinations were scheduled along the three trimester phases (9-13, 20-24, 32-34 gestation weeks) and puerperium (40-55 days after delivery). Two vaginal swabs were collected at each time point, to assess the vaginal microbiome profiling (by Nugent score and 16S rRNA gene sequencing) and the vaginal metabolic composition (1H-NMR spectroscopy). During pregnancy, the vaginal microbiome underwent marked changes, with a significant decrease in overall diversity, and increased stability. Over time, we found a significant increase of Lactobacillus and a decrease of several genera related to bacterial vaginosis (BV), such as Prevotella, Atopobium and Sneathia. It is worth noting that the levels of Bifidobacterium spp. tended to decrease at the end of pregnancy. At the puerperium, a significantly lower content of Lactobacillus and higher levels of Gardnerella, Prevotella, Atopobium, and Streptococcus were observed. Women receiving an intrapartum antibiotic prophylaxis for Group B Streptococcus (GBS) were characterized by a vaginal abundance of Prevotella compared to untreated women. Analysis of bacterial relative abundances highlighted an increased abundance of Fusobacterium in women suffering a first trimester abortion, at all taxonomic levels. Lactobacillus abundance was strongly correlated with higher levels of lactate, sarcosine, and many amino acids (i.e., isoleucine, leucine, phenylalanine, valine, threonine, tryptophan). Conversely, BV-associated genera, such as Gardnerella, Atopobium, and Sneathia, were related to amines (e.g., putrescine, methylamine), formate, acetate, alcohols, and short-chain fatty-acids (i.e., butyrate, propionate).

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“…Moreover, the most frequently described harmful change affecting the vaginal microbiome in women of reproductive age, bacterial vaginosis (BV), is characterized by a decrease in relative Lactobacillus abundance and an increase in anaerobic bacteria, primarily Gardnerella vaginalis (Fredricks et al, 2005). The presence of BV in early pregnancy was confirmed to be related to several adverse pregnancy outcomes, including preterm delivery (Petricevic et al, 2014), premature rupture of membranes (Purwar et al, 2001), recurrent miscarriage (Jiao et al, 2021), spontaneous abortion (Isik et al, 2016), and PID (Bagnall and Rizzolo, 2017). Although, the potential link between Gardnerella abundance and the incidence of TP has not been fully elucidated, we propose the following possible mechanism.…”

Section: Discussionmentioning

confidence: 99%

“…While the most abundant vaginal bacteria are Lactobacilli , other genera, including Gardnerella , Prevotella , Bifidobacterium , Atopobium , Megasphaera , Sneathia , and Anaerococcus also play important roles as part of the vaginal microbiome ( Huttenhower et al, 2012a ; Methé́ et al, 2012b ). 16S rRNA gene sequencing has been widely used to assess bacterial diversity and composition ( Woo et al., 2008 ; Siddiqui et al., 2011 ; Jiao et al., 2021 ). This tool can help clinicians to find uncultivable bacteria and evaluate new pathogens that could serve as biomarkers in various disease settings ( Yoshimura et al., 2011 ).…”

Section: Introductionmentioning

confidence: 99%

“…As a result, large efforts have been made to assess the bacterial composition and diversity of the healthy versus abnormal vaginal microbiome ( Franasiak and Scott, 2015 ; Moreno and Simon, 2019 ). In addition, vaginal microbial dysbiosis was shown to be associated with several female reproductive disorders, including premature delivery ( Kumar et al., 2021 ), premature rupture of membranes ( Amabebe et al., 2016 ), infertility ( Sharma et al., 2014 ), PID ( Rosca et al., 2020 ), in vitro fertilization (IVF) failure ( Karaer et al., 2021 ), and recurrent miscarriage ( Jiao et al., 2021 ). It is therefore of outmost importance to understand the vaginal microbiological characteristics of pregnant women, which are especially poorly described early on in pregnancy when fetal location is unknown, and represents a critical period for the early diagnosis of TP.…”

Section: Introductionmentioning

confidence: 99%

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Association Between Vaginal Gardnerella and Tubal Pregnancy in Women With Symptomatic Early Pregnancies in China: A Nested Case-Control Study

Zhang

Chen

et al. 2022

Front. Cell. Infect. Microbiol.

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The early diagnosis and treatment of ectopic pregnancy (EP) remains a major challenge. Despite a known link between vaginal microbiota and female reproductive health, few studies have focused on the association between vaginal microbiota and pregnancy location. This nested case-control study aimed to characterize the vaginal microbiota in tubal pregnancy (TP) among symptomatic women in early pregnancy. Women with symptomatic early pregnancy of unknown location (PUL) were included in this study. 16S rDNA gene sequencing was performed to assess vaginal microbial diversity and relative abundance. Machine learning and multivariate logistic regression were also used to evaluate the association between Gardnerella and TP. The results indicate that the vaginal microbiome in TP was more diverse (Shannon, p < 0.05) and was different in composition to that of women with intrauterine pregnancy (IUP) (weighted Unifrac, R = 0.08, p = 0.01). The genus Gardnerella was significantly enriched in TP. The XGBoost analysis was able to classify Gardnerella-induced TP more reliably (AUC = 0.621). Moreover, after adjusting potential confounders, our results indicate a robust association between Gardnerella and TP (as a continuous variable, adjusted OR: 12.0, 95% CI: 2.1–67.4, p < 0.01; as a categorical variable (≥0.85%), and adjusted OR: 4.2, 95% CI: 2.0–8.8, p < 0.01). In conclusion, we found that higher virginal Gardnerella levels were associated with TP in women with symptomatic early pregnancy.

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Alteration of vaginal microbiota in patients with recurrent miscarriage

Cited by 17 publications

References 29 publications

An Altered Microbiota in the Lower and Upper Female Reproductive Tract of Women with Recurrent Spontaneous Abortion

An Altered Microbiota in the Lower and Upper Female Reproductive Tract of Women with Recurrent Spontaneous Abortion

A Deep Look at the Vaginal Environment During Pregnancy and Puerperium

Association Between Vaginal Gardnerella and Tubal Pregnancy in Women With Symptomatic Early Pregnancies in China: A Nested Case-Control Study

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