Alterations and Correlations in Microbial Community and Metabolome Characteristics in Generalized Aggressive Periodontitis

Abstract

This study aimed to characterize the microbial community and metabolic profiles in generalized aggressive periodontitis (AgP) using 16S ribosomal RNA (rRNA) gene high-throughput sequencing and gas chromatography-mass spectrometry (GC-MS). A total of 146 subgingival plaque samples and 50 gingival crevicular fluid (GCF) samples were collected from 24 patients with AgP and 10 periodontally healthy subjects (PH). Striking differences were observed in subgingival microbiome and GCF metabolomics between patients with AgP and PH, but not between samples with different probing depths (PDs). Metabolomics analysis combined with enrichment analysis showed that periodontitis significantly altered the concentration of compounds associated with biosynthesis of amino acids (e.g., alanine, leucine, isoleucine, and valine), galactose metabolism (e.g., myo-inositol, galactose, glucose, and hexitol), and pyrimidine metabolism (e.g., uracil, uridine, beta alanine, and thymine). Correlation analysis showed that the genera with significant difference between AgP and PH were usually significantly correlated with more metabolites, such as Aggregatibacter, Rothia, Peptostreptococcaceae_[XI][G-5], and Bacteroidaceae_[G-1]. While glucose and oxoproline had the most significant correlations with microorganisms. Our results revealed distinct microbial communities and metabolic profiles between AgP and PH. The significant correlation between microbial taxa and metabolites suggested the possible mechanisms for periodontitis. Our results also provided effective approaches for detecting periodontal disease and managing periodontitis.

Discrimination of Bacterial Community Structures among Healthy, Gingivitis, and Periodontitis Statuses through Integrated Metatranscriptomic and Network Analyses

“…For example, the inositol phosphate metabolism and nitrogen metabolism pathways were found to be active only in H and G sites. A recent study of metabolomic profiles in periodontitis showed that inositol levels of gingival crevicular fluid were significantly higher in periodontally healthy subjects than in patients with periodontitis ( 36 ). Furthermore, Hoffman et al noted that nitrogen metabolism prevailed in a Prevotella -dominant bacterial community ( 37 ).…”

Structure and Function of Oral Microbial Community in Periodontitis Based on Integrated Data

“…The datasets from nine publications (Griffen et al, 2012;Galimanas et al, 2014;Bizzarro et al, 2016;Califf et al, 2017;Chen et al, 2018;Peŕez-Chaparro et al, 2018;Wei et al, 2019;Liu et al, 2020;Shi et al, 2020) were divided into two groups-periodontal disease (PD) and healthy control (HC)-according to our predetermined criteria. The mean age of the HC group was 34.4 ± 7.1 years, and the mean age of the PD group was 43.7 ± 8.2 years.…”

The Microbiota Profile Analysis of Combined Periodontal-Endodontic Lesions Using 16S rRNA Next-Generation Sequencing

“…One possible mechanism that Clostridium contributed to destruction is degrading Type IV collagen, which is the major component of basement membranes, via proteinases [56]. Several researchers have identified the abnormal accumulation of Peptostreptococcaceae in both chronic periodontitis and aggressive periodontitis [57][58][59][60][61]. In this study, we firstly reported Peptostreptococcaceae is rich in RE-PU.…”