Alterations in activity and mRNA expression of acetylcholinesterase in the liver, kidney and gill of common carp exposed to atrazine and chlorpyrifos

Xing, Houjuan; Wu, Hongda; Sun, Gang; Zhang, Ziwei; Xu, Shiwen; Shu, Li

doi:10.1016/j.etap.2012.11.004

Cited by 43 publications

(20 citation statements)

References 28 publications

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“…In fish, a decrease in AChE activity causes acetylcholine accumulation within synapses, leading to the impairment of important functions such as swimming, feeding and general behaviour (Glusczak et al 2006). Further, AChE inhibition may be associated with cell damage and lower AChE expression in the nervous system (Xing et al 2013;Zhang de et al 2013). It has been reported that AChE enzyme activity and its mRNA transcription decreased after exposure of chlorpyrifos in common carp brain (Xing et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Thein vitroandin vivoeffects of chlorpyrifos on acetylcholinesterase activity of rainbow trout brain

Topal

Şişecioğlu

Atamanalp

et al. 2015

Journal of Applied Animal Research

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Chlorpyrifos is widely used to control agricultural pests associated with fruit, nut and vegetable crops, despite its toxic effects and potential brain alterations in aquatic organisms. This study was carried out to determine the in vivo and in vitro effects of chlorpyrifos on rainbow trout brain acetylcholinesterase (AChE) enzyme activity. The fish were exposed to 2.25 µg/L (25% of 96 h LC 50 ), 4.5 µg/L (50% of 96 h LC 50 ) and 6.75 µg/L (75% of 96 h LC 50 ) of chlorpyrifos for 24, 48, 72 and 96 h. In vitro studies, inhibition constants (K i ) and half-maximal inhibitory concentration (IC 50 ) values for chlorpyrifos were determined by Lineweaver-Burk graphs and by plotting activity percentage vs.[I], respectively. Enzyme activities were determined by a colorimetric method. In fish exposed to chlorpyrifos 2.25 and 4.5 µg/L, enzyme inhibition was not observed (p > 0.05); however, chlorpyrifos exposure to 6.75 µg/L for 72 and 96 h caused a significant decrease in enzyme activity (p < 0.05). The IC 50 value of chlorpyrifos was found to be 30 µg/L. These results show that chlorpyrifos may cause direct cellular injury in the brain, and suggest that AChE may be used as a bioindicator for toxicological studies.

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Section: Discussionmentioning

confidence: 99%

Thein vitroandin vivoeffects of chlorpyrifos on acetylcholinesterase activity of rainbow trout brain

Topal

Şişecioğlu

Atamanalp

et al. 2015

Journal of Applied Animal Research

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“…Such activity could influence normal cellular function, due to inhibited nervous control (Cestèle and Catterall, 2000). Some branchial cells may downregulate their expression of genes or protein/enzyme synthesis, which may reduce AChE and MAO activity (Xing et al, 2013). Furthermore, a stressed organism may try to escape its aquatic environment which is contaminated by an A. flos-aquae bloom and aphantoxins.…”

Section: Effects Of Aphantoxin-exposure In Zebrafish Gillsmentioning

confidence: 99%

“…In addition, the reduced AChE may be due to the decreased synthesis or expression of AChE in cholinergic neurons induced by the neurotoxicity of aphantoxins or PSPs, which may impact nerve conduction involving neurotransmitters (Xing et al, 2013). Therefore, aphantoxins may influence the structure and function of the gill by reducing AChE activities, which might regulate the cholinergic conduction by neurotransmitters in the gills of zebrafish (Jindal and Kaur, 2014).…”

Section: Inhibition Of Ache Activity In Aphantoxin-exposed Zebrafish mentioning

confidence: 99%

Respiratory toxicity of cyanobacterial aphantoxins from Aphanizomenon flos-aquae DC-1 in the zebrafish gill

et al. 2016

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“…This was because the discourage of AChE activity is connect directly with the mechanism of toxic action of OPs. Furthermore, AChE was often used as an indicator of OPs exposure and physiological impact in exposed animals [33].…”

Section: International Journal Of Chemical Engineering and Applicatiomentioning

confidence: 99%

Bioconcentration and Antioxidant Status Responses in Zebrafish (Danio Rerio) Under Atrazine Exposure

Ghazie¹,

Sawafi²,

Yan³

2013

IJCEA

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Abstract-The present study was planned designed to investigate the chronic impacts of herbicide atrazine exposure on stress biomarkers acetylcholinesterase activity (AChE) and oxidative stress responses in brain of zebrafish (Danio rerio), and determine the bioconcentration of atrazine in whole body of fish. Chronic exposure to atrazine unveiled a markedly discourage in the activity of AChE. However, significant increase in the activities of catalase (CAT) and superoxide dismutase (SOD) and both influenced by atraizine, and CAT was over sensitivity to atrazine compared with SOD. The highest bioconcentration factor (BCF) of atrazine in the fish treated was (12.8543×10 4 and 13.5891×10 4 ) after 24h exposure to (0.957 and 1.913 mg L -1 ) and 13.238×10 4 , after 25 d exposure to 0.638 mg L −1 of atrazine respectively. This study showed that the zebrafish have ability to bioaccumulate of the herbicide atrazine rapidly. atrazine accumulation in zebrafish has a private importance due to the implication of atrazine in chronically health problems, as well as the information contained in our study is useful for understand the mechanism of atrazine induced oxidative stress in fish.

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Alterations in activity and mRNA expression of acetylcholinesterase in the liver, kidney and gill of common carp exposed to atrazine and chlorpyrifos

Cited by 43 publications

References 28 publications

Thein vitroandin vivoeffects of chlorpyrifos on acetylcholinesterase activity of rainbow trout brain

Thein vitroandin vivoeffects of chlorpyrifos on acetylcholinesterase activity of rainbow trout brain

Respiratory toxicity of cyanobacterial aphantoxins from Aphanizomenon flos-aquae DC-1 in the zebrafish gill

Bioconcentration and Antioxidant Status Responses in Zebrafish (Danio Rerio) Under Atrazine Exposure

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