Alterations in adenyl cyclase activity and glucose utilization of Bordetella pertussis-sensitized mouse spleen

Ortez, Richard A.; Seshachalam, Dutta; Szentivanyi, Andor

doi:10.1016/0006-2952(75)90341-x

Cited by 14 publications

(4 citation statements)

References 23 publications

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“…tain tissues by B. pertussis (14,17). However, this decrease was also demonstrable in the response to PGE, and fluoride, and hence is not an effect at the fl-receptor level, but rather at some distal site (14,17). In addition, our study with rat reticulocyte membranes and other studies (7,13) show that this altered response is not generalized, but is restricted to certain tissues.…”

Section: Resultssupporting

confidence: 52%

“…The results are expressed as percentage of maximal isoproterenol-stimulated enzyme activity (group III, 1,650 pmol of cyclic AMP per mg ofprotein per 10 min; group IV, 1,890 pmol of cyclic AMP per mg ofprotein per 10 min; group V, 1, 750 pmol of cyclic AMP per mg ofprotein per 10 min) and are the mean of triplicate determinations. tain tissues by B. pertussis (14,17). However, this decrease was also demonstrable in the response to PGE, and fluoride, and hence is not an effect at the fl-receptor level, but rather at some distal site (14,17).…”

Section: Resultsmentioning

confidence: 88%

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Bordetella pertussis does not induce beta-adrenergic blockade

Hewlett¹,

Spiegel

Wolff

et al. 1978

Infect Immun

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Bordetella pertussis organisms induce histamine sensitivity and diminish the normal hyperglycemic response to epinephrine in experimental animals. These effects have been attributed to ,8-adrenergic blockade. However, under conditions in which the decrease in epinephrine-induced hyperglycemia after B. pertussis administration was demonstrable, there was no change in rat reticulocyte ,8adrenergic receptor number or affinity measured by iodohydroxybenzylpindolol binding or in isoproterenol-stimulated adenylate cyclase activity. Therefore, there was no generalized,-adrenergic blockade induced by B. pertussis. The observed effects can be explained by the hypersecretion of insulin resulting from B. pertussis administration.

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Section: Resultssupporting

confidence: 52%

Section: Resultsmentioning

confidence: 88%

Bordetella pertussis does not induce beta-adrenergic blockade

Hewlett¹,

Spiegel

Wolff

et al. 1978

Infect Immun

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“…However, the reaction is more complex than that of beta adrenergic blockade, since the ability of prostaglandin E1 to increase cAMP is also inhibited. It is thus possible that LPF has a direct effect on adenylate cyclase, the activity of which appears to be decreased in the spleens of pertussis-treated mice (39). An alternative hypothesis is that cGMP metabolism is affected, with a consequent reciprocal effect on cAMP.…”

Section: Phase I Cells Of B Pertussis and Their Products Induce A Numentioning

confidence: 99%

Isolation and properties of the leukocytosis- and lymphocytosis-promoting factor of Bordetella pertussis.

Morse¹,

Morse²

1976

The Journal of Experimental Medicine

166

105

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In a previous report on the effects of the leukocytosis-and lymphocytosispromoting factor (LPF) 1 ofBordetella pertussis on cyclic adenosine 3':5'-monophosphate (cAMP) metabolism, a method for isolation of highly active LPF was presented briefly (1). In the present communication, the results of further studies on the isolation and chemical, immunochemical, and biological properties of LPF are described. It has been found that a single moiety is responsible for the induction in mice of leukocytosis with both lymphocytosis and granulocytosis, histamine sensitization, and hypoglycemia and unresponsiveness to the hyperglycemic effects of epinephrine. In confirmation of our preliminary report (2), LPF has been found to be clearly distinct from the hemagglutinating pili of B. pertussis, the properties of which are also described. Materials and MethodsB. pertussis Cultures. Strain NIH 114 (3779B), which has been utilized in continuing studies in this laboratory was also employed in the present investigations. Lyophilizod samples, obtained through the courtesy of Dr. Charles Manclark, Bureau of Biologics, Bethesda, Md., were reconstituted in sterile distilled H~O and plated on Bordet-Gengou medium (Clinical Sciences Inc., Whippany, N. J.). The 2-day growth at 36°C on one Petri dish was harvested in 10 ml of 2% casamino acids (technical grade; Difco Laboratories, Detroit, Mich.), and 1 ml of the suspension was inoculated into 100 ml of liquid medium in i liter Blake bottles. After 2 days of incubation at 36°C, 10 ml of the seed culture (approximately 5 × 10 x° organJma) was inoculated into 1 liter of liquid medium in Povitsky bottles which were then incubated at 35-36°C. In order to provide maximum surface area for aeration, all bottles were placed in the horizontal position during incubation. In the case of the 100 ml cultures, the depth of the medium was 1.0 cm and the surface area 218 cm2; the values for the 1-1iter cultures were 2.5 cm and 580 cm 2, respectively.The liquid medium was that previously described (3) except for the omission of an anion exchange resin which was found to be unnecessary for either optimal growth, maintenance of the Phase I state, or maximal production of LPF. Culture density was assessed with the National Institutes of Health opacity standard, and the criteria utilized to determine whether the organisms were Phase I included: a homogenous population of small, gram-negative coccobacilli and

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“…From earlier studies it became clear that hypoglycemia is obtained within 6 h after the administration of B. pertussis vaccine, whereas a decrease in cAMP results after 4 days when the mice are maximally sensitive to histamine anaphylaxis (9,11). Moreover, histamine sensitivity closely responds to changes in the content of cAMP (9). The increased sensitivity of mice to histamine appears within 24 to 28 h after injection ofB.…”

Section: De Wildt Kreeftenberg and Nijkampmentioning

confidence: 99%

Impaired autonomic responsiveness of the cardiovascular system of the rat induced by a heat-labile component of Bordetella pertussis vaccine

Wildt¹,

Kreeftenberg²,

Nijkamp³

1983

Infect Immun

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Bordetella pertussis vaccination induces severe impairment of the autonomic responsiveness of the cardiovascular system in rats. The vasodilation after P2adrenoceptor stimulation with salbutamol as well as the negative chronotropic action induced by the muscarinic receptor stimulant arecoline were inhibited 4 days after vaccination. Moreover, basal blood pressure values appeared to be significantly lower in B. pertussis-vaccinated rats compared with control animals. These effects were dependent upon the bacterial strain used. Differences in pharmacological activity due to strain differences paralleled variations in the content of lymphocytosis-promoting factor of the vaccine. The inhibitory effects were absent after the administration of vaccine heated for 1 h at 80°C, implicating an important role for a heat-labile component, e.g., lymphocytosis-promoting factor, and not for a heat-stable constituent, e.g., endotoxin (lipopolysaccharide). Previous studies indicate that some early biological effects elicited by B. pertussis vaccine can be attributed to lipopolysaccharide, whereas late induced effects are mainly brought about by lymphocytosis-promoting factor. For that reason a role for lipopolysaccharide might be excluded because 5 h after vaccination no disturbances of the autonomic nervous system were observed. We conclude that B. pertussis vaccination induces autonomic hyporesponsiveness due to a heatlabile component that is assumed to be lymphocytosis-promoting factor.

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Alterations in adenyl cyclase activity and glucose utilization of Bordetella pertussis-sensitized mouse spleen

Cited by 14 publications

References 23 publications

Bordetella pertussis does not induce beta-adrenergic blockade

Bordetella pertussis does not induce beta-adrenergic blockade

Isolation and properties of the leukocytosis- and lymphocytosis-promoting factor of Bordetella pertussis.

Impaired autonomic responsiveness of the cardiovascular system of the rat induced by a heat-labile component of Bordetella pertussis vaccine

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