Alterations in growth of tissue‐cultured tansy (Tanacetum vulgare L.) treated with antibiotics

Abstract

Effects of three antibiotics (cefotaxime, rifampicin and gentamicin) were tested on in vitro shoots cultures of tansy (Tunacetum vulgare L.). These antibiotics were selected because endophytic bacteria isolated from the in vitro shoot cultures of tansy showed that all bacteria were Gram-negative and their growth was reduced by these three antibiotics. Five isolates were Enterobacteriaceae, three were fluorescent Pseudomonas, and two were aerobic bacteria. Increased concentrations of antibiotics caused usually linear or quadratic changes on the initiation of shoot growth, shoot number, growth rate, and shoot height. These changes and changes in pH of the culture media were tansy genotype-dependent following treatments with gentamicin. Also, the treatment with rifampicin or cefotaxime showed a genotype-dependent effect, because they resulted in significantly higher percentage of rooted plants in one of the three tansy genotypes tested. The growth rate and length of shoots were reduced in the media containing both gentamicin and rifampicin, but less so than in media containing both gentamicin and cefotaxime.

Effects of β-lactam antibiotics, auxins, and cytokinins on shoot regeneration from callus cultures of two hybrid aspens, Populus tremuloides × P. tremula and P. x canescens × P. gradidentata

“…In oregano, CEF (250 mg l -1 ) increased the water content of shoot cultures to a statistically significant degree but the shoots did not appear hyperhydric, as visually evaluated, and the changes were modest in absolute terms (88.5 vs. 90% water content, Perry et al 1999). In tansy, the frequency of shoot hyperhydricity was increased when CEF (6.25-100 mg l -1 ) was added to the micropropagation medium, but similar effects were apparent for all of the antibiotics evaluated (CEF, rifampicin, gentamicin) despite their distinct chemical structures and toxicity mechanisms (Keskitalo et al 1998). Within the Populus genus, when MS medium with CEF and CARB was used for shoot regeneration from transgenic calli of black poplar shoot differentiation was delayed by hyperhydricity 'phenomena'.…”

Biocompatible Magnetite Nanoparticles Trapped at the Air/Water Interface

“…Traditionally, Eucalyptus leaves and vapour from boiling water with dried leaves have been used in many countries, including Tunisia, as analgesic, anti‐inflammatory and antipyretic remedies for symptoms of respiratory infections and toothache. Recent studies confirm the analgesic, anti‐inflammatory and antipyretic properties of this oil 14–16. Essential oils of Eucalyptus species have been proven effective against many bacteria and fungi pathogenic to humans 9, 17–20.…”

Composition chimique, propriétés antimicrobiennes et activités sur les tiques de l'huile essentielle d'Eucalyptus tereticornis Sm

“…Au cours des dix dernières années, de nombreuses études ont tenté de démontrer la synergie d'action des constituants d'huile essentielle sur les différentes sortes de cellules végétales ou animales dans le but de vaincre les réticences que suscite encore l'application de ces essences en pharmacologie et dans les essais cliniques [6][7][8]. À cet égard, l'utilisation de ces substances naturelles présente un avantage certain sur les produits de synthèse, qui se révèlent toxiques et parfois même cancérigènes, aussi bien pour les cellules animales que végétales [9].…”