Alterations in the activities of ornithine and histidine decarboxylases provoked by testosterone in mice

Grahn, Birgitta; Henningsson, Stig; Kahlson, G.; Rosengren, E.

doi:10.1111/j.1476-5381.1973.tb08228.x

Cited by 37 publications

(13 citation statements)

References 29 publications

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“…It would appear that the high putrescine formation of the male kidney is mediated by testosterone, as the administration of this hormone to female mice is known to elevate ornithine decarboxylase activity (Grahn et al 1973), and castration of male mice resulted in a reduction of the activity of this enzyme as presently shown.…”

Section: Discussionmentioning

confidence: 94%

“…The activity of ornithine decarboxylase was low during the first three weeks after birth in both sexes with a rapid and steep increase after weaning in the male but not in the female. It may be conjectured that putrescine functionally substitutes for histamine in the male kidney as has been suggested to apply to certain growing tissues (Russell & Snyder, 1968;Grahn et al 1973).…”

Section: Discussionmentioning

confidence: 99%

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Biosynthesis of histamine and putrescine in mice during post‐natal development and its hormone dependence.

Henningsson¹,

Rosengren²

1975

The Journal of Physiology

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1. Histidine decarboxylase and ornithine decarboxylase activities were determined in mouse kidney and liver during post‐natal development. 2. The content of histamine, putrescine, spermidine and spermine was examined in kidney and liver and in the urine of adult male and female mice. 3. Histamine formation by the kidney was high in both sexes when determined a few days after birth but decreased during weaning. Thereafter, a distinct sex difference was established in that in the female kidney the level of histidine decarboxylase rose several‐fold during adolescence while in the male the level was still further reduced. 4. Putrescine formation by mouse kidney was low in both sexes up to three weeks of age whereafter the amine formation in the male increased conspicuously whereas that of the female kidney remained low. 5. The observed sex differences in tissue enzyme activities were reflected in concomitant differences in the amount of the diamines excreted in the urine. 6. No correlation was found between the actual enzyme levels and the assayed tissue content of histamine, putrescine, spermidine and spermine. 7. Following gonadectomy, the activities of both decarboxylases were significantly altered. Ornithine decarboxylase activity of male kidney and histidine decarboxylase activity of female kidney were strikingly reduced. 8. In the mouse liver, the two decarboxylases displayed no changes comparable with that of the kidney during development.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Biosynthesis of histamine and putrescine in mice during post‐natal development and its hormone dependence.

Henningsson¹,

Rosengren²

1975

The Journal of Physiology

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“…Purification-Adult male ddY mice were given testosterone propionate at a dose of 100 mg/kg body weight by single subcutaneous injection of a solution of 10 mg/ml in sesame oil 3 days prior to sacrifice, in order to induce ODC in the kidneys (12)(13)(14)(15)(16). All purification procedures were carried out at 4°C.…”

Section: Resultsmentioning

confidence: 99%

Molecular Properties of Ornithine Decarboxylase from Mouse Kidney: Detailed Comparison with Those of the Enzyme from Rat Liver1

Kitani¹,

Fujisawa²

1988

The Journal of Biochemistry

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Ornithine decarboxylase (ODC) was purified about 2,000-fold from the kidney of androgen-treated mice and its molecular properties were examined and compared with those of the enzyme from rat liver. The purified enzyme showed two protein staining bands on SDS-polyacrylamide gel electrophoresis, corresponding to Mr of about 54,000 and 52,000. The apparent Mr of the enzyme determined by gel filtration was 57,000 in the presence of 0.25 M NaCl and 110,000 in its absence. The apparent Km value for L-ornithine was about 0.1 mM in the absence of NaCl and 0.7 mM in the presence of 0.25 M NaCl. Thus, salts appeared to cause subunit dissociation and also an increase in the Km value for the substrate. Putrescine and D-ornithine acted as inhibitors competing with the substrate. Antizyme from the rat liver inhibited the activities of the mouse enzyme and the rat enzyme similarly. The mouse and the rat enzymes exhibited a very similar immunological cross-reactivity to rabbit antibody raised against the mouse enzyme but, when the antibody directed against the rat enzyme was used, the cross-reactivity of the rat enzyme was higher than that of the mouse enzyme. Thus, the molecular properties of mouse ODC were very similar to those of the rat enzyme.

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“…In particular, renal ornithine decarboxylase (ODC) activity is remarkably higher in males than in females, and the treatment of females with testosterone elicits a dramatic increase of ODC activity in renal tissue (Henningsson et al 1978, Melatinou et al 1987, Sánchez-Capelo et al 1994, mediated by transcriptional, translational, and post-translational mechanisms (Seely et al 1982, Kontula et al 1984, Berger & Watson 1989. On the other hand, renal histidine decarboxylase is up-regulated by estrogens and repressed by androgens (Grahn et al 1973, Middleton et al 1987, Middleton & Bulfield 1988. In contrast, little is known about the influence of sex hormones on the regulation of DDC in murine tissues.…”

Section: Introductionmentioning

confidence: 99%

Opposite sexual dimorphism of 3,4-dihydroxyphenylalanine decarboxylase in the kidney and small intestine of mice

López-Contreras

Galindo²,

López‐García³

et al. 2007

Journal of Endocrinology

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3,4-Dihydroxyphenylalanine decarboxylase (DDC; also known as L-amino acid decarboxylase) is involved in the synthesis of dopamine, norepinephrine, and serotonin, and also acts as an androgen receptor co-regulator protein. In contrast to other amino acid decarboxylases that are modulated by sex hormones, little is known about the influence of these hormones on DDC regulation. In the present work, we studied the influence of gender in the expression of DDC in different mouse tissues. Among the different organs studied, including brain, liver, kidney, intestine, heart, adrenal gland, and skeletal muscle, only kidney and small intestine showed a sex-dependent dimorphism in DDC expression. In the kidney, levels of DDC activity, DDC mRNA, and protein were remarkably higher in females than in males. On the contrary, in the small intestine, male mice displayed higher levels of DDC activity than females but they did not correlate precisely with mRNA levels. This dimorphism was dependent on androgens, since male castration and treatment of female mice with testosterone propionate, oppositely affected DDC levels in kidney and small intestine. However, estrogen ablation or treatment with estradiol did not significantly affect DDC activity in these tissues. Immunocytochemical analysis revealed that DDC was mainly located in the proximal straight tubular cells of the kidney and in the cytoplasm of enterocytes. These data and the fact that renal DDC inversely correlated with renal sodium reabsorption suggest that renal and intestinal gender dimorphism in DDC could be related to sexrelated differences in sodium balance observed between males and females.

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Alterations in the activities of ornithine and histidine decarboxylases provoked by testosterone in mice

Cited by 37 publications

References 29 publications

Biosynthesis of histamine and putrescine in mice during post‐natal development and its hormone dependence.

Biosynthesis of histamine and putrescine in mice during post‐natal development and its hormone dependence.

Molecular Properties of Ornithine Decarboxylase from Mouse Kidney: Detailed Comparison with Those of the Enzyme from Rat Liver1

Opposite sexual dimorphism of 3,4-dihydroxyphenylalanine decarboxylase in the kidney and small intestine of mice

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