Alterations in the Activities of Few Lysosomal Enzymes in the Kidney and Intestine of Mice Exposed to Low Concentrations of a PCB, Aroclor 1254 under in vitro condition

Abstract

Polychlorinated biphenyls (PCBs) are industrial POPs which have been released into the environment resulting in widespread and persistent contamination. The aim of the present investigation was to determine the alterations in the activities of few lysosomal enzymes (acid phosphatase, α-galactosidase, βgalactosidase and β-glucuronidase) in lysosomal cellular sub-fractions of kidney and intestine of mice after the exposure to different concentrations of Aroclor 1254 (25 µg/ml, 50 µg/ml and 100 µg/ml) for two different time intervals (15 and 30 minutes). The study was aimed to answer a couple of hypothesis set in null form, that (a) Aroclor 1254 does not have any direct effect on the selected lysosomal enzymes under in vitro condition, and (b) Aroclor 1254 does not exhibit any organ-specific toxic effects on the activities of selected lysosomal enzymes in kidney and intestine of mice under in vitro condition. The overall results of the present study revealed that the even very low concentration of Aroclor 1254 has direct and organ-specific toxic effects on the activities of selected lysosomal enzymes in kidney and intestine of mice, cancelling both the hypotheses. Therefore, the results of present study were suggested that Aroclor 1254 have the ability to induce the lysosomal destabilization by altering the activities of different lysosomal enzymes which may lead to apoptosis or necrosis in different tissues of mice.

Effects of Low dose Aroclor 1254 on Few Lysosomal Biomarker Enzymes from Liver and Brain of Mice under in vitro condition

“…In in vitro conditions, Aroclor 1254 comes in direct contact with the lysosomal enzymes and inhibit the activities while under in vivo condition, the toxicant is possibly comes into contact with the lysosomal enzymes via indirect ways. It is possible that the PCB passes through the cellular metabolic pathways and produces the hazardous metabolites which come into contact with these enzymes and induce the observed effects in the enzyme activity [7,25]. The overall results of the present study revealed that these alterations in the activities of lysosomal enzymes of mice liver and brain may create the cellular disturbances that affect the normal functional activities of lysosomes such as intracellular digestion and recycling of macromolecules [2].…”