Alterations in the gut microbiome and metabolome profiles of septic rats treated with aminophylline

Abstract

The treatment of sepsis remains a major challenge worldwide. Aminophylline has been shown to have anti-inflammatory effects; however, the role of aminophylline in sepsis, a disease characterized by immune dysregulation, is unknown. In this study, we combined microbiome sequencing and metabolomic assays to investigate the effect of aminophylline administration on the intestinal flora and metabolites in septic rats. Sixty SD rats were randomly divided into three groups: a sham-operated (SC) group, a sepsis model (CLP) group and a CLP + aminophylline treatment (Amino) group. The intestinal flora and metabolic profile of rats in the CLP group were significantly different than those of the SC group, while aminophylline administration resulted in a return to a state similar to healthy rats. Differential abundance analysis showed that aminophylline significantly back-regulated the abundance of Firmicutes, unidentified_Bacteria, Proteobacteria, Lactobacillus, Escherichia-Shigella and other dominant bacteria (P < 0.05) and altered chenodeoxycholic acid, isolithocholic acid and a total of 26 metabolites (variable importance in the projection (VIP) > 1, P < 0.05). In addition, we found that there were significant correlations between differential metabolites and bacterial genera of the Amino and CLP groups. For example, Escherichia-Shigella was associated with 12 metabolites, and Lactobacillus was associated with two metabolites (P < 0.05), suggesting that differences in the metabolic profiles caused by aminophylline were partly dependent on its influence on the gutmicrobiome. In conclusion, this study identified a novel protective mechanism whereby aminophylline could regulate disordered intestinal flora and metabolites in septic rats.

Lactobacillus and Allobaculum mediates the improvement of vascular endothelial dysfunction during hypertension with TaohongSiwu decoction combined with Dubosiella newyorkensis

“…The Study found that Lactobacillus was the main group of bacteria involved in the 7α-dehydroxylation reaction of cholic acid to DCA [ 22 ]. Lactobacillus showed positive regulation with chenodeoxycholic acid [ 23 ], which was consistent with our experimental results. Futhermore, Lactobacillus possessed bile salt hydrolase (BSH) activity, which promoted the hydrolysis of bile acids in the gut by regulating BSH activity [ 24 , 25 ].…”

Modulation of Caecal Microbiota and Metabolome Profile in Salmonella-Infected Broilers by Phage Therapy

“…This genus has been related to acetic acid production and its influence on lipid metabolism [51]. Lipids regulate biological processes such as immunity and inflammation [52]. In addition, Bacteroides has been associated with the metabolism of bile acids, proteins, fats and carbohydrates [51].…”

Investigation of Gut Microbiota Disorders in Sepsis and Sepsis Complicated with Acute Gastrointestinal Injury Based on 16S rRNA Genes Illumina Sequencing

“…29 Previous studies have found that the abundance of Firmicutes in the sepsis group is reduced. 30,31 Moreover, its abundance increased in the sepsis group after treatment with related drugs. 30,32 The Bacteroidota, also known as Bacteroidetes, is an anaerobic bacterium that plays a role in immunity and 33 It is usually associated with gut inflammation, and its abundance is also different before and after the treatment of sepsis.…”

“…30,31 Moreover, its abundance increased in the sepsis group after treatment with related drugs. 30,32 The Bacteroidota, also known as Bacteroidetes, is an anaerobic bacterium that plays a role in immunity and 33 It is usually associated with gut inflammation, and its abundance is also different before and after the treatment of sepsis. 34,35 In the Circos diagram, Firmicutes and Bacteroidota is a dominant species at the phylum level.…”