Alterations of hepatocyte function with free radical generators and reparation or prevention with coffee polyphenols

Abstract

Liver diseases are linked in the majority of cases to oxidative stress that antioxidants could neutralize with reducing liver injury. Chlorogenic acid, a coffee polyphenol, possesses antioxidant prosperities. The aim of this study was to evaluate in vitro preventive and corrective effects of cholorogenic acid in hepatocyte toxicity induced by free radicals. Hepatocytes were isolated from adult male Wistar rats. To determine corrective effects and reparation, cells were first exposed to two free radical generators (hydrogen peroxide/iron sulfate for hydroxyl radical formation, and phenazine methosulfate/nicotinamide adenine dinucleotide for superoxide anion formation) for 12H and thereafter treated by chlorogenic acid (1 and 10 μM final concentration) for another 12H. To show preventive effects, cells were pretreated by chlorogenic acid and thereafter exposed to free radical generators. Hepatocyte proliferation, glucose uptake, ATP contents, membrane fluidity and integrity, and intracellular redox status were investigated after 24H culture. The results showed that chlorogenic acid reversed the decrease in cell proliferation, glucose uptake and ATP levels, the increased LDH release and the reduced membrane fluidity and restored the oxidant/antioxidant status under oxidative stress. When pre-treated with chlorogenic acid, hepatocytes became very resistant to oxidative conditions and cellular homeostasis was maintained. In conclusion, chlorogenic acid displayed not only corrective but also preventive effects in hepatocytes exposed to oxidative stress and could be beneficial in patients with or at risk of liver diseases.

Prospective Application of Tannic Acid in Acetaminophen (APAP)-Induced Acute Liver Failure

“…The relative oxidation stress levels in MHs cultured in the APAP-MHs, [TA + (APAP-MHs)], and [TA + (APAP-MHs) + TA] groups were 100% ± 5.4%, 94% ± 5.3%, and 89% ± 3.2%, respectively ( Figure 3 D), suggesting that 1 μg/mL of TA not only slightly prevents oxidative stress before toxicity but also reduces oxidative stress after cell toxicity significantly. This finding aligns with that of a previous study [ 23 ] that reported the antioxidative effects of TA, indicating that it reduces oxidative stress and free radical damage.…”

Inverse Association of Coffee with Liver Cancer Development: An Updated Systematic Review and Meta-analysis

“…The protective effects of coffee are postulated due to multiple ingredients. Phenolic acids and caffeine may directly impact liver carcinogenesis via inhibiting proliferation of HCC cells, suppressing progression of HCC and preventing oxidative damage to hepatocytes [42][43][44]. It is also postulated that coffee reduces insulin resistance and improves glucose metabolism which may also play a role in slowing the progression of liver disease [45].…”

Coffee consumption by type and risk of digestive cancer: a large prospective cohort study

“…Compounds such as phenolic acids 27,28 and caffeine 29 have also been shown to have chemopreventive properties, including in liver carcinogenesis 38 . These compounds have been shown to inhibit the proliferation of HCC cell lines in vitro and suppress the progression of HCC in vivo 30 , while chlorogenic acid has been shown to prevent oxidative damage in hepatocytes 31 . Instant coffee, in comparison with ground and decaffeinated coffee, has been demonstrated to have higher levels of those biochemical compounds 3,4 , which therefore could partly explain the more marked reduced risk of HCC in our study.…”